Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-27T23:30:47.191Z Has data issue: false hasContentIssue false
  • English
  • Français

Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes

Published online by Cambridge University Press:  01 June 2009

Catherine A. O'Sullivan ,
Patrick J. Joyce ,
Teresa Sloan  and
Alan G. Shattock
Catherine A. O'Sullivan
Affiliation:
Department of Zoology and University College Dublin, Belfield, Dublin 4, Irish Republic
Patrick J. Joyce
Affiliation:
Department of Zoology and University College Dublin, Belfield, Dublin 4, Irish Republic
Teresa Sloan
Affiliation:
Department of Zoology and University College Dublin, Belfield, Dublin 4, Irish Republic
Alan G. Shattock
Affiliation:
Department of Medical Microbiology, University College Dublin, Belfield, Dublin 4, Irish Republic
Get access Rights & Permissions [Opens in a new window]

Summary

A direct capture enzyme-linked immunosorbent assay (ELISA) was developed to measure elevated polymorphonuclear granulocyte (PMN) antigens using horseradish peroxidase (EC 1.11.1.7) conjugated rabbit polyclonal anti-PMN antisera and a monoclonal antibody specific for PMN cells. Optical densities obtained in the ELISA were used to predict the cell counts of milk samples. Predicted counts were not significantly different from actual somatic cell counts (SCC). In a total of 156 bovine milk samples the correlation coefficient between somatic cell counting, taking > 500000 cells/ml as being indicative of mastitis, and the assay was 0·94, yielding an assay sensitivity of 95·2% and a specificity of 97·3%. In further trials the ELISA could detect elevated PMN antigens in milk with SCC as low as 100000 cells/ml. The results indicate that the monoclonal antibody based direct ELISA has excellent potential in the detection and determination of bovine mastitis.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 2 , May 1992 , pp. 123 - 133
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Campbell, R. C. 1967 Statistics for Biologists. Cambridge: Cambridge University PressGoogle Scholar
Carlson, G. P. & Kaneko, J. J. 1973 Isolation of leukocytes from bovine peripheral blood. Proceedings of the Society for Experimental Biology and Medicine 142 853–856CrossRefGoogle ScholarPubMed
Cotter, T. G., Spears, P. & Henson, P. M. 1981 A monoclonal antibody inhibiting human neutrophil chemotaxis and degranulation. Journal of Immunology 127 1355–1360CrossRefGoogle ScholarPubMed
Cullen, G. A. 1966 Cells in milk. A review. Veterinary Bulletin 36 337–346Google Scholar
Eberhart, R. J., Harmon, R. J., Jasper, D. E., Natzke, R. P., Nickerson, S. C., Reneau, J. K., Row, E. H., Smith, K. L. & Spencer, S. B. 1987 In Current Concepts of Bovine Mastitis, 3rd edn.USA: National Mastitis CouncilGoogle Scholar
Fernando, R. S., Rindsig, R. B. & Spahr, S. L. 1982 Electrical conductivity of milk for detection of mastitis. Journal of Dairy Science 65 659–664CrossRefGoogle ScholarPubMed
Hart, G. 1980 Screening to control infectious diseases: evaluation of control programs for gonorrhea and syphilis. Reviews of Infectious Diseases 2 701–712CrossRefGoogle ScholarPubMed
Kitchen, B. J. 1981 Review of the progress of Dairy Science: Bovine mastitis: milk compositional changes and related diagnostic tests. Journal of Dairy Research 48 167–188CrossRefGoogle ScholarPubMed
Kitchen, B. J., Middleton, G., Durward, I. G., Andrews, R. J. & Salmon, M. C. 1980 Mastitis diagnostic tests to estimate mammary gland epithelial cell damage. Journal of Dairy Science 63 978–983CrossRefGoogle ScholarPubMed
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Lightner, J. K., Miller, G. Y., Hueston, W. D. & Dorn, C. R. 1988 Food animal economics: estimation of the costs of mastitis, using National Animal Health Monitoring System and milk somatic cell count data. Journal of the American Veterinary Medical Association 192 1410–1413Google Scholar
Meek, A. H., Barnum, D. A. & Newbould, F. H. S. 1980 Use of total and differential somatic cell counts to differentiate potentially infected from potentially non-infected quarters and cows and between herds of various levels of infection. Journal of Food Protection 43 10–14CrossRefGoogle ScholarPubMed
Ruffo, G. 1968 The role of the cell count in the diagnosis of chronic staphylococcal mastitis. Industria Latte 4 278–287Google Scholar
St Groth, S. F. De & Scheidegger, D. 1980 Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35 1–21CrossRefGoogle ScholarPubMed
Schalm, O. W., Carroll, E. J. & Jain, N. C. 1971 Bovine Mastitis. Philadelphia, PA: Lea and FebigerGoogle Scholar
Schultze, W. D. 1985 Developments in the identification of diseased udder quarters or cows. Kieler Milchwirtschaftliche Forschungsberichte 37 319–328Google Scholar
Smith, A. M. & Tedder, R. S. 1981 Development of an enzyme-linked immunosorbent assay for hepatitis Be antigen and antibody. Journal of Virological Methods 3 1–11CrossRefGoogle ScholarPubMed
Smith, K. L. 1983 Mastitis control: a discussion. Journal of Dairy Science 66 1790–1794CrossRefGoogle ScholarPubMed
Towbin, H., Staehelin, T. & Gordon, J. 1979 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences, USA 76 4350–4354CrossRefGoogle ScholarPubMed