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Characterization of biofilms and antimicrobial resistance of coagulase-negative Staphylococcus species involved with subclinical mastitis

Published online by Cambridge University Press:  17 May 2021

Márcia Silva Francisco ,
Ciro César Rossi ,
Maria Aparecida Vasconcelos Paiva Brito ,
Marinella Silva Laport ,
Elaine Menezes Barros  and
Marcia Giambiagi-deMarval
Márcia Silva Francisco
Affiliation:
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Ciro César Rossi
Affiliation:
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Maria Aparecida Vasconcelos Paiva Brito
Affiliation:
Embrapa Gado de Leite, Juiz de Fora, MG, Brazil
Marinella Silva Laport
Affiliation:
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Elaine Menezes Barros
Affiliation:
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Marcia Giambiagi-deMarval*
Affiliation:
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
*
Author for correspondence: Marcia Giambiagi-deMarval, Email: marciagm@micro.ufrj.br
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Abstract

Biofilm formation is a central feature to guarantee staphylococcal persistence in hosts and is associated with several diseases that are difficult to treat. In this research paper, biofilm formation and antimicrobial susceptibility were investigated in staphylococcal strains belonging to several species. These strains were isolated from the milk of cows with subclinical mastitis and most of them were coagulase-negative, with the prevalence of Staphylococcus chromogenes. High genetic diversity was observed among the strains by pulsed field gel electrophoresis. Antimicrobial resistance was assessed by disk diffusion and more than 50% of the strains were resistant to ampicillin and penicillin G, with multi-resistance profiles (13.6%) also being observed. Most strains (65.9%) formed biofilms when cultivated in BHI supplemented with 1% glucose. Most strains (72.7%) carried the intercellular adhesion gene (icaA), while less than half (36.3%) carried the biofilm-associated protein gene (bap). Concentrations of up to 10xMIC of erythromycin and tetracycline were not sufficient to suppress cell viability in preformed biofilms. Our results revealed that a genetically diverse group of biofilm-forming Staphylococcus species can be involved in subclinical mastitis. Since high antimicrobial concentrations cannot eradicate biofilm cells in vitro, their use in dairy animals may be ineffective in controlling infections, while supporting selection of resistant microorganisms. These data reinforce the need for alternative therapies aiming at disrupting biofilms for effective disease control.

Keywords

Antimicrobial resistancebiofilmstaphylococcisubclinical mastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 2 , May 2021 , pp. 179 - 184
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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Footnotes

*

These authors contributed equally to this work.

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