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Effect of the lactoperoxidase system on lipoprotein lipase activity and lipolysis in milk

Published online by Cambridge University Press:  01 June 2009

Lennart Ahrné  and
Lennart Björck
Lennart Ahrné
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, S-750 07 Uppsala 7, Sweden
Lennart Björck
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, S-750 07 Uppsala 7, Sweden
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Summary

The lactoperoxidase/SCN-/H2O2 system (LPS) was found to inhibit lipoprotein lipase (LPL) in milk. Inhibition was related to the amount of SCN-/H2O2 added to milk. Lipolysis was unaltered until LPL activity was decreased to <40% of the original activity. When LPL was inhibited further, decreased lipolysis was observed. Purified LPL was also inhibited by LPS, at similar concentrations of SCN-/H2O2 as in milk. It was shown that non enzymically prepared OSCN- inhibited LPL at concentrations comparable to those produced by the LPS. Cysteine could eliminate the effect of LPS on LPL. The sulphydryl reagents dithiobisnitrobenzoic acid and Na tetrathionate had no effect on LPL activity.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 4 , November 1985 , pp. 513 - 520
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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References

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