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The effects of acetate and of pyruvate on the pathways of glucose catabolism in lactating mammary tissue: 1. Rat tissue

Published online by Cambridge University Press:  01 June 2009

R. W. Smith  and
R. F. Glascock
R. W. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
R. F. Glascock
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
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Summary

A study was made of the changes in the pathways of glucose catabolism in slices of lactating rat mammary gland which occurred when acetate or pyruvate was added to the medium.

When acetate was added there was an increase in the rates of oxidation of the C(2) and C(6) atoms of glucose but little change in that of the C(1) atom. The amounts of these carbon atoms which were incorporated into fatty acids, lactic acid and glyceride glycerol were reduced, but the results showed that the relative proportion of the glucose converted to these substances which passed through the pentose phosphate pathway was increased.

When pyruvate was added, the rates of oxidation of the C(1), C(2) and C(6) atoms were all reduced to about the same extent. The incorporation of the glucose carbon into fatty acids was almost abolished and its incorporation into lactic acid and glyceride glycerol was very much reduced. The results showed that most of the glucose incorporated into these compounds under these conditions passed through the pentose phosphate pathway.

Evidence is presented which suggests that the triose phosphates are not in complete isotopic equilibrium in lactating rat mammary tissue.

These results are discussed in relation to the existence of 2 pools of acetyl coenzyme A, one intra- and the other extra-mitochondrial, and to the possible inhibition of certain steps of the glycolytic pathway.

Type
Original Articles
Information
Journal of Dairy Research , Volume 36 , Issue 3 , October 1969 , pp. 455 - 467
Copyright
Copyright © Proprietors of Journal of Dairy Research 1969

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References

REFERENCES

Abraham, S. & Chaikoff, I. L. (1959). J. biol. Chem. 234, 2246.CrossRefGoogle Scholar
Abraham, S., Hirsch, P. F. & Chaikoff, I. L. (1954). J. biol. Chem. 211, 31.CrossRefGoogle Scholar
Balmain, J. H., Folley, S. J. & Glascock, R. F. (1952). Biochem. J. 52, 301.CrossRefGoogle Scholar
Balmain, J. H., Folley, S. J. & Glascock, R. F. (1954). Biochem. J. 56, 234.CrossRefGoogle Scholar
Bethencourt, A. V., Matos, O. E. & Shipp, J. C. (1966). Metabolism 15, 847.CrossRefGoogle ScholarPubMed
Dils, R. & Popják, G. (1962). Biochem. J. 83, 41.CrossRefGoogle Scholar
Duncombe, W. G. & Glascock, R. F. (1956). Biochem. J. 63, 326.CrossRefGoogle Scholar
Glascock, R. F. (1954). Isotopic Gas Analysis for Biochemists. New York: Academic Press Inc.Google Scholar
Greenbaum, A. L. & Darby, F. J. (1964). Biochem. J. 91, 307.CrossRefGoogle Scholar
Hirsch, P. F., Baruch, H. & Chaikoff, I. L. (1954). J. biol. Chem. 210, 785.CrossRefGoogle Scholar
Katz, J., Landau, B. R. & Bartsch, G. E. (1966). J. biol. Chem. 241, 727.CrossRefGoogle Scholar
Katz, J. & Wood, H. G. (1960). J. biol. Chem. 235, 2165.CrossRefGoogle Scholar
Krebs, H. A. & Henseleit, K. (1932). Hoppe-Seyler's Z. physiol. Chem. 210, 33.CrossRefGoogle Scholar
Ladd, J. N. & Nossal, P. M. (1954). Aust. J. exp. Biol. med. Sci. 32, 523.CrossRefGoogle Scholar
McLean, P. (1958). Biochim. biophys. Acta 30, 303.CrossRefGoogle Scholar
McLean, P. (1960). Biochim. biophys. Acta 37, 296.CrossRefGoogle Scholar
McLean, P. (1964). Biochem. J. 90, 271.CrossRefGoogle Scholar
Marks, P. A. & Horecker, B. L. (1956). J. biol. Chem. 218, 327.CrossRefGoogle Scholar
Moore, J. H. (1962). J. Dairy Res. 29, 141.CrossRefGoogle Scholar
Newsholme, E. A., Randle, P. J. & Manchester, K. L. (1962). Nature, Lond. 193, 270.CrossRefGoogle Scholar
Price, V. E. & Levintow, L. (1952). Biochem. Prep. 2, 22.Google Scholar
Smith, R. W. & Phillips, S. H. (1969). Int. J. appl. Radiat. Isotopes 20, 553.CrossRefGoogle Scholar
Smith, S., Easter, D. J. & Dils, R. (1966). Biochim. biophys. Acta 125, 445.CrossRefGoogle Scholar
Walters, E. & Mclean, P. (1967). Biochem. J. 105, 615.CrossRefGoogle Scholar
Williamson, J. R. (1965). J. biol. Chem. 240, 2308.CrossRefGoogle Scholar