Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-07-03T01:32:54.687Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of xanthosine treatment on gene expression of mammary glands in early lactating goats

Published online by Cambridge University Press:  29 August 2018

Ratan K Choudhary ,
Shanti Choudhary ,
Devendra Pathak ,
Rahul Udehiya ,
Ramneek Verma ,
Sandeep Kaswan ,
Arpan Sharma ,
Dhiraj Gupta ,
Mrigank Honparkhe  and
Anthony V Capuco
Ratan K Choudhary*
Affiliation:
School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Science University (GADVASU), Ludhiana – 101004, India
Shanti Choudhary
Affiliation:
School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Science University (GADVASU), Ludhiana – 101004, India
Devendra Pathak
Affiliation:
Department of Veterinary Anatomy, GADVASU, Ludhiana – 101004, India
Rahul Udehiya
Affiliation:
Department of Veterinary Surgery and Radiology, GADVASU, Ludhiana – 101004, India
Ramneek Verma
Affiliation:
School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Science University (GADVASU), Ludhiana – 101004, India
Sandeep Kaswan
Affiliation:
Department of Livestock Production & Management, GADVASU, Ludhiana – 101004, India
Arpan Sharma
Affiliation:
Department of Livestock Production & Management, GADVASU, Ludhiana – 101004, India
Dhiraj Gupta
Affiliation:
Department of Veterinary Medicine, GADVASU, Ludhiana – 101004, India
Mrigank Honparkhe
Affiliation:
Department of Veterinary Gynaecology & Obstetrics, GADVASU, Ludhiana – 101004, India
Anthony V Capuco
Affiliation:
Animal Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA
*
*For correspondence; e-mail: vetdrrkc@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

This study examined the hypothesis that xanthosine (XS) treatment would promote mammary-specific gene expression and stem cell transcripts and have a positive influence on milk yield of dairy goats. Seven primiparous Beetal goats were assigned to the study. Five days after kidding, one gland (either left or right) was infused with XS (TRT) twice daily for 3 d and the other gland with no XS infusion served as a control (CON). Mammary biopsies were collected at 10 d and RNA was isolated. Gene expression analysis of milk synthesis genes, mammary stem/progenitor cell markers, cell proliferation and differentiation markers were performed using real time quantitative PCR (RT-qPCR). Results showed that the transcripts of milk synthesis genes (BLG4, CSN2, LALBA, FABP3, CD36) and mammary stem/progenitor cell markers (ALDH1 and NR5A2) were increased in as a result of XS treatment. Average milk yield in TRT glands was increased marginally (approximately ~2% P = 0·05, paired t-test) per gland relative to CON gland until 7 wk. After 7 wk, milk yield of TRT and CON glands did not differ. Analysis of milk composition revealed that protein, lactose, fat and solids-not-fat percentages remained the same in TRT and CON glands. These results suggest that XS increases expression of milk synthesis genes, mammary stem/progenitor cells and has a small effect on milk yield.

Keywords

Goatmammary glandxanthosinemammary gene expressionmilk yield
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 3 , August 2018 , pp. 288 - 294
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baldassarre, H, Deslauriers, J, Neveu, N & Bordignon, V 2011 Detection of endoplasmic reticulum stress markers and production enhancement treatments in transgenic goats expressing recombinant human butyrylcholinesterase. Transgenic Research 20 1265–1272Google Scholar
Bouillez, A, Gnemmi, V, Gaudelot, K, Hemon, B, Ringot, B, Pottier, N, Glowacki, F, Butruille, C, Cauffiez, C, Hamdane, M, Sergeant, N, Van Seuningen, I, Leroy, X, Aubert, S & Perrais, M 2014 MUC1-C nuclear localization drives invasiveness of renal cancer cells through a sheddase/gamma secretase dependent pathway. Oncotarget 5 754–763Google Scholar
Capuco, AV, Evock-Clover, CM, Minuti, A & Wood, DL 2009 In vivo expansion of the mammary stem/progenitor cell population by xanthosine infusion. Experimental Biology and Medicine 234 475–482Google Scholar
Chen, W, Wang, H, Tao, S, Zheng, Y, Wu, W, Lian, F, Jaramillo, M, Fang, D & Zhang, DD 2013 Tumor protein translationally controlled 1 is a p53 target gene that promotes cell survival. Cell Cycle 12 2321–2328Google Scholar
Choudhary, RK & Capuco, AV 2012 In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment. BMC Cell Biology 13 14Google Scholar
Choudhary, RK, Li, RW, Evock-Clover, CM & Capuco, AV 2013 Comparison of the transcriptomes of long-term label retaining-cells and control cells microdissected from mammary epithelium: an initial study to characterize potential stem/progenitor cells. Frontiers in Oncology 3 21Google Scholar
Choudhary, RK, Choudhary, S, Kaur, H & Pathak, D 2016 Expression of putative stem cell marker, hepatocyte nuclear factor 4 alpha, in mammary gland of water buffalo. Animal Biotechnology 27 182–189Google Scholar
Choudhary, S & Choudhary, RK 2017 Rapid and efficient method of total RNA isolation from milk fat for transcriptome analysis of mammary gland. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences https://doi.org/10.1007/s40011-017-0955-8Google Scholar
Colitti, M & Parillo, F 2013 Immunolocalization of estrogen and progesterone receptors in ewe mammary glands. Microscopy Research and Technique 76 955–962Google Scholar
De Stefano, I, Zannoni, GF, Prisco, MG, Fagotti, A, Tortorella, L, Vizzielli, G, Mencaglia, L, Scambia, G & Gallo, D 2011 Cytoplasmic expression of estrogen receptor beta (ERβ) predicts poor clinical outcome in advanced serous ovarian cancer. Gynecologic Oncology 122 573–579Google Scholar
Dontu, G, Jackson, KW, McNicholas, E, Kawamura, MJ, Abdallah, WM & Wicha, MS 2004 Role of notch signaling in cell-fate determination of human mammary stem/progenitor cells. Breast Cancer Research 6 R605–R615Google Scholar
Ginestier, C, Hur, MH, Charafe-Jauffret, E, Monville, F, Dutcher, J, Brown, M, Jacquemier, J, Viens, P, Kleer, CG, Liu, S, Schott, A, Hayes, D, Birnbaum, D, Wicha, MS & Dontu, G 2007 ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell 1 555–567Google Scholar
Gonçalves, CF, Morais, MO, de CG Alencar, R, Mota, ED, Silva, TA, Batista, AC & Mendonça, EF 2011 Expression of Ki-67 and MUC1 in mucoepidermoid carcinomas of young and adult patients: prognostic implications. Experimental and Molecular Pathology 90 271–275Google Scholar
Hart, IC & Morant, SV 1980 Roles of prolactin, growth hormone, insulin and thyroxine in steroid-induced lactation in goats. Journal of Endocrinology 84 343–351Google Scholar
Heng, J-CD, Feng, B, Han, J, Jiang, J, Kraus, P, Ng, J-H, Orlov, YL, Huss, M, Yang, L, Lufkin, T, Lim, B & Ng, H-Hl 2010 The nuclear receptor Nr5a2 can replace oct4 in the reprogramming of murine somatic cells to pluripotent cells. Cell Stem Cell 6 167–174Google Scholar
Kapila, N, Kishore, A, Sodhi, M, Sharma, A, Kumar, P, Mohanty, aK, Jerath, T & Mukesh, M 2013 Identification of appropriate reference genes for qRT-PCR analysis of heat-stressed mammary epithelial cells in riverine buffaloes (Bubalus bubalis). ISRN Biotechnology 2013 1–9Google Scholar
Lee, H-S, Crane, GG, Merok, JR, Tunstead, JR, Hatch, NL, Panchalingam, K, Powers, MJ, Griffith, LG & Sherley, JL 2003 Clonal expansion of adult rat hepatic stem cell lines by suppression of asymmetric cell kinetics (SACK). Biotechnology and Bioengineering 83 760–771Google Scholar
Li, Y, Yi, H, Yao, Y, Liao, X, Xie, Y, Yang, J, Yan, Z, Wang, L, Lu, S, Kuang, Y, Gu, M, Fei, J, Wang, Z & Huang, L 2011 The cytoplasmic domain of MUC1 induces hyperplasia in the mammary gland and correlates with nuclear accumulation of β-catenin. PloS One 6 e19102Google Scholar
Meyer, MJ, Capuco, AV, Boisclair, YR & Van Amburgh, ME 2006 Estrogen-dependent responses of the mammary fat pad in prepubertal dairy heifers. Journal of Endocrinology 190 819–827Google Scholar
Osińska, E, Wicik, Z, Godlewski, MM, Pawłowski, K, Majewska, A, Mucha, J, Gajewska, M & Motyl, T 2014 Comparison of stem/progenitor cell number and transcriptomic profile in the mammary tissue of dairy and beef breed heifers. Journal of Applied Genetics 55 383–395Google Scholar
Rambhatla, L, Bohn, SA, Stadler, PB, Boyd, JT, Coss, RA & Sherley, JL 2001 Cellular senescence: ex vivo p53-dependent asymmetric cell kinetics. Journal of Biomedicine and Biotechnology 1 28–37Google Scholar
Rauner, G & Barash, I 2014 Xanthosine administration does not affect the proportion of epithelial stem cells in bovine mammary tissue, but has a latent negative effect on cell proliferation. Experimental Cell Research 328 186–196Google Scholar
Safayi, S, Theil, PK, Hou, L, Engbaek, M, Nørgaard, JV, Sejrsen, K & Nielsen, MO 2010 Continuous lactation effects on mammary remodeling during late gestation and lactation in dairy goats. Journal of Dairy Science 93 203–217Google Scholar
Schindelin, J, Arganda-Carreras, I, Frise, E, Kaynig, V, Longair, M, Pietzsch, T, Preibisch, S, Rueden, C, Saalfeld, S, Schmid, B, Tinevez, J-Y, White, DJ, Hartenstein, V, Eliceiri, K, Tomancak, P & Cardona, A 2012 Fiji: an open-source platform for biological-image analysis. Nature Methods 9 676–682Google Scholar
Spitsberg, VL, Matitashvili, E & Gorewit, RC 1995 Association and coexpression of fatty-acid-binding protein and glycoprotein CD36 in the bovine mammary gland. European Journal of Biochemistry 230 872–878Google Scholar
Thornton, B & Basu, C 2011 Real-time PCR (qPCR) primer design using free online software. Biochemistry and Molecular Biology Education 39 145–154Google Scholar
Vandesompele, J, De Preter, K, Pattyn, F, Poppe, B, Van Roy, N, De Paepe, A & Speleman, F 2002 Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3 1–11Google Scholar
Welsh, AW, Lannin, DR, Young, GS, Sherman, ME, Figueroa, JD, Henry, NL, Ryden, L, Kim, C, Love, RR, Schiff, R & Rimm, DL 2012 Cytoplasmic estrogen receptor in breast cancer. Clinical Cancer Research 18 118–126Google Scholar
Ye, J, Coulouris, G, Zaretskaya, I, Cutcutache, I, Rozen, S & Madden, TL 2012 Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics 13 134Google Scholar
Yohe, TT, Tucker, HLM, Parsons, CLM, Geiger, AJ, Akers, RM & Daniels, KM 2016 Short communication: initial evidence supporting existence of potential rumen epidermal stem and progenitor cells. Journal of Dairy Science 99 7654–7660Google Scholar
Supplementary material: PDF

Choudhary et al. supplementary material

Tables S1 and S2

Download Choudhary et al. supplementary material(PDF)
PDF 143.2 KB