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Production of goat milk protein hydrolysate enriched in ACE-inhibitory peptides by ultrafiltration

Published online by Cambridge University Press:  08 July 2014

Francisco Javier Espejo-Carpio ,
Raúl Pérez-Gálvez ,
María del Carmen Almécija ,
Antonio Guadix  and
Emilia M. Guadix
Francisco Javier Espejo-Carpio*
Affiliation:
Department of Chemical Engineering, University of Granada, 18071 Granada, Spain
Raúl Pérez-Gálvez
Affiliation:
Department of Chemical Engineering, University of Granada, 18071 Granada, Spain
María del Carmen Almécija
Affiliation:
Department of Chemical Engineering, University of Granada, 18071 Granada, Spain
Antonio Guadix
Affiliation:
Department of Chemical Engineering, University of Granada, 18071 Granada, Spain
Emilia M. Guadix
Affiliation:
Department of Chemical Engineering, University of Granada, 18071 Granada, Spain
*
*For correspondence; e-mail: fjespejo@ugr.es
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Abstract

A global process for the production of goat milk hydrolysates enriched in angiotensin converting enzyme (ACE) inhibitory peptides was proposed. Firstly, the protein fractions (caseins and whey proteins) were separated by ultrafiltration through a 0·14 μm ceramic membrane. The casein fraction obtained in the retentate stream of the above filtration step was subsequently hydrolysed with a combination of subtilisin and trypsin. After 3 h of reaction, the hydrolysate produced presented an IC50 of 218·50 μg/ml, which represent a relatively high ACE inhibitory activity. Finally, this hydrolysate was filtered through a 50 kDa ceramic membrane until reaching a volume reduction factor of 3. The permeate produced presented an improvement of more than 30% in the ACE inhibitory activity. In contrast, the retentate was concentrated in larger and inactive peptides which led to a decrease of more than 80% in its inhibitory activity. The process suggested in this work was suitable to obtain a potent ACE inhibitory activity product able to be incorporated into food formulas intended to control or lower blood pressure. Moreover, the liquid product could be easily stabilised by spray dried if it would be necessary.

Keywords

Goat milk proteinenzymatic hydrolysisACE-inhibitory activitymembrane ultrafiltrationpeptide identification
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 4 , November 2014 , pp. 385 - 393
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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