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Proline-specific peptidases of Streptococcus cremoris AM2

Published online by Cambridge University Press:  01 June 2009

Mary Booth ,
William J. Donnelly ,
Ide Ni Fhaoláin ,
P. Vincent Jennings  and
Gerard O'Cuinn
Mary Booth
Affiliation:
Department of Biochemistry, University College, Galway, Ireland
William J. Donnelly
Affiliation:
The National Dairy Product Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
Ide Ni Fhaoláin
Affiliation:
Department of Life Sciences, Regional Technical College, Galway, Ireland
P. Vincent Jennings
Affiliation:
Department of Life Sciences, Regional Technical College, Galway, Ireland
Gerard O'Cuinn
Affiliation:
Department of Life Sciences, Regional Technical College, Galway, Ireland
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Summary

The present study identifies proline-specific peptidases found in Streptococcus cremoris AM2. These activities were present prcdominantly in the cytoplasmic fraction of the cells with minor amounts in cell membrane fraction. No evidence vvas found for such activities in either the extracellular fluid used to grow the cells, in the wash fraction or in the cell wall fraction. The involvement of these enzymes in release of free proline from proline-containing peptides is considered.

Type
Original Articles
Information
Journal of Dairy Research , Volume 57 , Issue 1 , February 1990 , pp. 79 - 88
Copyright
Copyright © Proprietors of Journal of Dairy Research 1990

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