Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-19T15:34:04.221Z Has data issue: false hasContentIssue false
  • English
  • Français

Serum lipoprotein stimulation of lipolysis and its relevance to free fatty acid development in bovine milk

Published online by Cambridge University Press:  01 June 2009

Malcolm Anderson  and
Eric C. Needs
Malcolm Anderson
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT, UK.
Eric C. Needs
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT, UK.
Get access Rights & Permissions [Opens in a new window]

Summary

The role of blood serum lipoproteins in stimulating milk lipolysis was investigated by adding blood serum and heparin to the milk of normal cows and cows in which elevated somatic cell counts were induced by intramammary infusion of Escherichia coli endotoxin and Staphylococcus aureus. There was considerable variation between individual milks in the extent to which lipolysis was stimulated. In some milks there was almost no response. For values obtained from 29 cows during the first 6 months of lactation a relationship was observed between free fatty acid (FFA) levels in the untreated and serum-stimulated samples (r = 0·776). FFA values were higher after 18 h at 4 °C in milks from infused quarters than in those from control quarters, but this difference was not entirely due to higher values from the infused quarters at 0 h. Both blood serum and heparin stimulated lipolysis in high cell count milks and control milks. The response to heparin was greater than that to blood serum, but the response to both was highly correlated with FFA levels in the untreated milks. There were no differences in the ability of skim-milks prepared from infused and control quarters to promote lipolysis in recombined milks containing sterilized homogenized milk fat globules as a source of substrate. Incubation of blood serum with 2 μg/ml trypsin for 1 h at 37 °C reduced the ability of the serum to promote lipolysis. The importance of lipase activation by blood serum lipoproteins in relation to milk lipolysis is discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 50 , Issue 3 , August 1983 , pp. 309 - 319
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, M. 1979 Enzyme immunoassay for measuring lipoprotein lipase activator in milk. Journal of Dairy Science 62 1380–1383CrossRefGoogle ScholarPubMed
Anderson, M. 1981 Inhibition of lipolysis in bovine milk by proteose peptone. Journal of Dairy Research 48 247–252CrossRefGoogle ScholarPubMed
Anderson, M. 1982 Factors affecting the distribution of lipoprotein lipase activity between serum and casein micelles in bovine milk. Journal of Dairy Research 49 51–59CrossRefGoogle ScholarPubMed
Astrup, H. N., SkrØvseth, O., Vik-Mo, L., Ekern, A. & Sola, E. 1977 Feed palmitic acid as a factor in milk lipolysis. Meieriposten 66 681–683Google Scholar
Castberg, H. B. & Solberg, P. 1974 The lipoprotein lipase and the lipolysis in bovine milk. Meieriposten 63 961–975Google Scholar
Clegg, R. A. 1980 Activation of milk lipase by serum proteins: possible role in the occurrence of lipolysis in raw bovine milk. Journal of Dairy Research 47 61–70CrossRefGoogle ScholarPubMed
Clegg, R. A. 1981 Lipoprotein lipase in the mammary gland and milk. Hannah Research Institute Report, 75–87.Google Scholar
Deeth, H. C. & Fitz-Gerald, C. H. 1975 Factors governing the susceptibility of milk to spontaneous lipolysis. International Dairy Federation Bulletin (Document 86) 24–34Google Scholar
De Rham, O. & Andrews, A. T. 1982 Qualitative and quantitative determination of proteolysis in mastitis milks. Journal of Dairy Research 49 587–596CrossRefGoogle ScholarPubMed
Downey, W. K. 1980 Review of the progress of Dairy Science. Flavour impairment from pre- and post-manufacture lipolysis in milk and dairy products. Journal of Dairy Research 47 237–252CrossRefGoogle Scholar
Driessen, F. M. & Stadhouders, J. 1974 A study of spontaneous rancidity. Netherlands Milk and Dairy Journal 28 130–145Google Scholar
Fitz-Gerald, C. H., Deeth, H. C. & Kitchen, B. J. 1981 The relationship between the levels of free fatty acids, lipoprotein lipase, carboxylesterase, N-acetyl-β-D-glucosaminidase, somatic cell count and other mastitis indices in bovine milk. Journal of Dairy Research 48 253–265CrossRefGoogle ScholarPubMed
Fleming, M. G. 1979 Lipolysis in bovine milk as affected by mechanical and temperature activation. Irish Journal of Food Science and Technology 3 111–129Google Scholar
Hoynes, M. C. T. & Downey, W. K. 1973 Relationship of the lipase and lipoprotein lipase activities of bovine milk. Biochemical Society Transactions 1 256–259CrossRefGoogle Scholar
Iverius, P. H., Lindahl, V., Egelrud, T. & Olivecrona, T. 1972 Effects of heparin on lipoprotein lipase from bovine milk. Journal of Biological Chemistry 247 6610–6616CrossRefGoogle ScholarPubMed
Jellema, A. 1975 Note on susceptibility of bovine milk to lipolysis. Netherlands Milk and Dairy Journal 29 45–152.Google Scholar
Jellema, A. 1980 Physiological factors associated with lipolytic activity in cow's milk International Dairy Federation Bulletion (Document 118) 33–40Google Scholar
Kinnunen, P. K. J., Jackson, R. L., Smith, L. C., Gotto, A. M. & Sparrow, J. T. 1977 Activation of lipoprotein lipase by native and synthetic fragments of human plasma apolipoprotein C-II. Proceedings of the National Academy of Sciences of the USA 74 4848–4851CrossRefGoogle ScholarPubMed
Koops, J. & Klomp, H. 1977 Rapid colorimetric determination of free fatty acids (lipolysis) in milk by the copper soap method. Netherlands Milk and Dairy Journal 31 56–74Google Scholar
Murphy, J. J., Connolly, J. F. & Headon, D. R. 1979 A study of factors associated with free fatty acid development in milk. Journal of Food Science and Technology 3 131–149Google Scholar
Newbould, F. H. S. & Neave, F. K. 1965 The recovery of small numbers of Staphylococcus aureus infused into the bovine teat cistern. Journal of Dairy Research 32 157–162CrossRefGoogle Scholar
Nilsson-Ehle, P. & Schotz, M. C. 1976 A stable, radioactive substrate emulsion for assay of lipoprotein lipase. Journal of Lipid Research 17 536–541CrossRefGoogle ScholarPubMed
Olivecrona, T. 1980 Biochemical aspects of lipolysis in bovine milk. International Dairy Federation Bulletin (Document 118) 19–25Google Scholar
Olivecrona, T. & Lindahl, U. 1969 The occurrence of a heparin-like glycosaminoglycan in bovine milk and its possible association with lipoprotein lipase. Acta Chemica Scandinavica 23 3587–3589CrossRefGoogle ScholarPubMed
Pettipher, G. L. & Rodrigues, U. M. 1981 Rapid membrane filtration epifluorescent microscopic technique for the direct enumeration of somatic cells in fresh and formalin-preserved milk. Journal of Dairy Research 48 239–246CrossRefGoogle Scholar
Randolph, H. E. & Erwin, R. E. 1974 Influence of mastitis on properties of milk. X Fatty acid composition. Journal of Dairy Science 57 865–868CrossRefGoogle ScholarPubMed
Salih, A. M. A. & Anderson, M. 1979 Observations on the influence of high cell count on lipolysis in bovine milk. Journal of Dairy Research 46 453–462CrossRefGoogle ScholarPubMed
Shipe, W. F., Senyk, G. F. & Fountain, K. B. 1980 Modified copper soap solvent extraction method for measuring free fatty acids in milk. Journal of Dairy Science 63 193–198CrossRefGoogle Scholar
Super, D. M., Palmquist, D. L. & Schanbacher, F. L. 1976 Relative activation of milk lipoprotein lipase by serum of cows fed varying amounts of fat. Journal of Dairy Science 59 1409–1413CrossRefGoogle ScholarPubMed