Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-19T05:46:56.707Z Has data issue: false hasContentIssue false
  • English
  • Français

Coagulase-negative staphylococci from non-mastitic bovine mammary gland: characterization of Staphylococcus chromogenes and Staphylococcus haemolyticus by antibiotic susceptibility testing and pulsed-field gel electrophoresis

Published online by Cambridge University Press:  09 November 2011

Mateja Pate ,
Irena Zdovc ,
Jana Avberšek ,
Matjaž Ocepek ,
Andrej Pengov  and
Ožbalt Podpečan
Mateja Pate*
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Irena Zdovc
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Jana Avberšek
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Matjaž Ocepek
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Andrej Pengov
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Ožbalt Podpečan
Affiliation:
Savinian Veterinary Policlinic, Celjska 3a, 3310 Žalec, Slovenia
*
*For correspondence; e-mail: mateja.pate@vf.uni-lj.si
Get access Rights & Permissions [Opens in a new window]

Abstract

During routine microbiological examination of milk samples from dairy cows without clinical signs of mastitis, quarter milk samples of 231 dairy cows from 12 herds were investigated for the presence of coagulase-negative staphylococci (CNS). The isolates were identified on the basis of colony morphology, Gram staining, catalase and coagulase test and the commercial kit, API Staph. CNS was detected in 29% (67/231) of the cows. A total of seven CNS species were identified with the most prevalent being Staphylococcus (Staph.) chromogenes (30%) and Staph. haemolyticus (28·8%), followed by Staph. simulans (11·2%), Staph. xylosus (11·2%), Staph. epidermidis (7·5%), Staph. hyicus (6·3%) and Staph. sciuri (5%). The predominant species, Staph. chromogenes and Staph. haemolyticus, were further characterized by antibiotic susceptibility testing using the agar disc diffusion method (Kirby-Bauer) and by pulsed-field gel electrophoresis (PFGE). Considerable resistance to ampicillin and penicillin was observed in both species. Isolates with identical or highly similar PFGE profiles were detected at the herd level despite a marked heterogeneity seen for both species. On the basis of somatic cell count, absence of clinical signs of inflammation and heterogeneity of genotypes, we assume that CNS isolated in this study could not be considered as important causative agents of the bovine mammary gland inflammation.

Keywords

Staphylococcus chromogenesStaphylococcus haemolyticusantibiotic susceptibility testingPFGEbovine milk
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 2 , May 2012 , pp. 129 - 134
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bal, EB, Bayar, S & Bal, MA 2010 Antimicrobial susceptibilities of coagulase-negative staphylococci (CNS) and streptococci from bovine subclinical mastitis cases. Journal of Microbiology 48 267–274CrossRefGoogle ScholarPubMed
Boddie, RL, Nickerson, SC, Owens, WE & Watts, JL 1987 Udder microflora in nonlactating heifers. Agri-Practice 8 22–25Google Scholar
Clinical and Laboratory Standards Institute 2011 Performance standards for antimicrobial susceptibility testing; twenty-first informational supplement. CLSI document M100-S21. Wayne PA, USA: Clinical and Laboratory Standards InstituteGoogle Scholar
De Vliegher, S 2004 Udder health in dairy heifers: Some epidemiological and microbiological aspects. Ghent: Faculteit Diergeneeskunde (PhD thesis)Google Scholar
Devriese, LA, Baele, M, Vaneechoutte, M, Martel, A & Haesebrouck, F 2002 Identification and antimicrobial susceptibility of Staphylococcus chromogenes isolates from intramammary infections of dairy cows. Veterinary Microbiology 87 175–82CrossRefGoogle ScholarPubMed
Devriese, LA & De Keyser, H 1980 Prevalence of different species of coagulase-negative staphylococci on teats and in milk samples from dairy cows. Journal of Dairy Research 47 155–158CrossRefGoogle ScholarPubMed
Fessler, AT, Billerbeck, C, Kadlec, K & Schwarz, S 2010 Identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis. Journal of Antimicrobial Chemotherapy 65 1576–1582CrossRefGoogle ScholarPubMed
Garcia, P, Benitez, R, Lam, M, Salinas, AM, Wirth, H, Espinoza, C, Garay, T, Depix, MS, Labarca, J & Guzmán, AM 2004 Coagulase-negative staphylococci: clinical, microbiological and molecular features to predict true bacteraemia. Journal of Medical Microbiology 53 67–72CrossRefGoogle ScholarPubMed
Gillespie, BE, Headrick, SI, Boonyayatra, S & Oliver, SP 2009 Prevalence and persistence of coagulase-negative Staphylococcus species in three dairy research herds. Veterinary Microbiology 134 65–72CrossRefGoogle ScholarPubMed
Jemeljanovs, A, Konosonoka, IH & Bluzmanis, J 2007 Coagulase negative staphylococcal mastitis in dairy farms of Latvia. In: Proceedings of the Heifer Mastitis Conference. Ghent, Belgium, pp. 44–45Google Scholar
Krause, R, Haberl, R, Wölfler, A, Daxböck, F, Auner, HW, Krejs, GJ, Wenisch, C & Reisinger, EC 2003 Molecular typing of coagulase-negative staphylococcal blood and skin culture isolates to differentiate between bacteremia and contamination. European Journal of Clinical Microbiology and Infectious Diseases 22 760–763CrossRefGoogle ScholarPubMed
Matthews, KR, Oliver, SP & King, SH 1990 Comparison of Vitek gram-positive identification system with API staph-trac system for species identification of staphylococci of bovine origin. Journal of Clinical Microbiology 28 1649–1651CrossRefGoogle ScholarPubMed
Matthews, KR, Harmon, RJ & Langlois, BE 1992 Prevalence of Staphylococcus species during the periparturient period in primiparous and multiparous cows. Journal of Dairy Science 75 1835–1839CrossRefGoogle ScholarPubMed
Myllys, V 1995 Staphylococci in heifer mastitis before and after parturition. Journal of Dairy Research 62 51–60CrossRefGoogle ScholarPubMed
Myllys, V, Asplund, K, Brofeldt, E, Hirvela-Koski, V, Honkanen-Buzalski, T, Junttila, J, Kulkas, L, Myllykangas, O, Niskanen, M, Saloniemi, H, Sandholm, M & Saranpaa, T 1998 Bovine mastitis in Finland in 1988 and 1995—changes in prevalence and antimicrobial resistance. Acta Veterinaria Scandinavica 39 119–126CrossRefGoogle ScholarPubMed
Nam, HM, Lim, SK, Kim, JM, Kang, HM, Moon, JS, Jang, GC, Kim, JM, Wee, SH, Joo, YS & Jung, SC 2010 Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis between 2003 and 2008 in Korea. Journal of Microbiology and Biotechnology 20 1446–1449CrossRefGoogle ScholarPubMed
National Committee for Clinical Laboratory Standards Institute 2002 Performance Standards for Antimicrobial Disk and Dillution Susceptibility Test for Bacteria Isolated from Animals; Informational Supplement. NCCLS document M31-A2Google Scholar
Oliver, SP, Gillespie, BE, Headrick, SJ, Lewis, MJ & Dowlen, HH 2005 Prevalence, risk factors and strategies for controlling mastitis in heifers during the periparturient period. International Journal of Applied Research in Veterinary Medicine 3 150–162Google Scholar
Rajala-Schultz, PJ, Torres, AH, DeGraves, FJ, Gebreyes, WA & Patchanee, P 2009 Antimicrobial resistance and genotypic characterization of coagulase-negative staphylococci over the dry period. Veterinary Microbiology 134 55–64Google Scholar
Persson, L, Strid, H, Tidefeld, U & Söderquist, B 2006 Phenotypic and genotypic characterization of coagulase-negative staphylococci isolated in blood from patients with haematological malignacies. European Journal of Clinical Microbiology and Infectious Diseases 25 299–301CrossRefGoogle Scholar
Pyörälä, S & Taponen, S 2009 Coagulase-negative staphylococci: emerging mastitis pathogens. Veterinary Microbiology 134 3–8CrossRefGoogle ScholarPubMed
Sampimon, OC, Barkema, HW, Berends, IM, Sol, J & Lam, TJ 2009 Prevalence and herd-level risk factors for intramammary infection with coagulase-negative staphylococci in Dutch dairy herds. Veterinary Microbiology 134 37–44CrossRefGoogle ScholarPubMed
Sampimon, OC, Lam, TJ, Mevius, DJ, Schukken, YH & Zadoks, RN 2011 Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine milk samples. Veterinary Microbiology 150 173–179CrossRefGoogle ScholarPubMed
Sawant, AA, Gillespie, BE & Oliver, SP 2009 Antimicrobial susceptibility of coagulase-negative Staphylococcus species isolated from bovine milk. Veterinary Microbiology 134 73–81CrossRefGoogle ScholarPubMed
Schukken, YH, González, RN, Tikofsky, LL, Schulte, HF, Santisteban, CG, Welcome, FL, Bennett, GJ, Zurakowski, MJ & Zadoks, RN 2009 CNS mastitis: nothing to worry about? Veterinary Microbiology 134 9–14CrossRefGoogle ScholarPubMed
Shimizu, A, Kloos, WE, Berkhoff, HA, George, CG & Ballard, DN 1997 Pulsed-field gel electrophoresis of Staphylococcus hyicus and Staphylococcus chromogenes genomic DNA and its taxonomic, epidemiologic and ecologic applications in veterinary medicine. The Journal of Veterinary Medical Science 59 443–450CrossRefGoogle ScholarPubMed
Taponen, S, Björkroth, J & Pyörälä, S 2008 Coagulase-negative staphylococci isolated from bovine extramammary sites and intramammary infections in a single dairy herd. Journal of Dairy Research 75 422–429CrossRefGoogle Scholar
Taponen, S & Pyörälä, S 2009 Coagulase-negative staphylococci as cause of bovine mastitis – not so different from Staphylococcus aureus? Veterinary Microbiology 134 29–36CrossRefGoogle Scholar
Taponen, S, Simojoki, H, Harveri, M, Larsen, D & Pyörälä, S 2006 Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Veterinary Microbiology 115 199–207CrossRefGoogle ScholarPubMed
Thorberg, BM, Kuhn, I, Aarestrup, FM, Brandstrom, B, Jonsson, P & Nielsson-Tham, ML 2006 Pheno- and genotyping of Staphylococcus epidermidis isolated from bovine milk and human skin. Veterinary Microbiology 115 163–172CrossRefGoogle ScholarPubMed
Timms, LL & Schultz, LH 1987 Dynamics and significance of coagulase-negative staphylococcal intrammary infections. Journal of Dairy Science 70 2648–2657CrossRefGoogle Scholar
Trinidad, P, Nickerson, SC & Alley, TK 1990 Prevalence of intramammary infection and teat canal colonization in unbred and primigravid dairy heifers. Journal of Dairy Science 73 107–114CrossRefGoogle ScholarPubMed