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Lactobacillus plantarum bacteriophages isolated from Kefir grains: phenotypic and molecular characterization

Published online by Cambridge University Press:  29 September 2009

Graciela De Antoni ,
Miriam Zago ,
Olga Vasek ,
Giorgio Giraffa ,
Domenico Carminati ,
Mariángeles Briggiler Marcó ,
Jorge Reinheimer  and
Viviana Suárez
Graciela De Antoni
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas, Universidad National de La Plata, La Plata, Argentina
Miriam Zago
Affiliation:
Agriculture Research Council, Research Centre for Forage and Dairy Productions, Lodi, Italy
Olga Vasek
Affiliation:
Facultad de Ciencias Exactas, Universidad Nacional del Nordeste, Corrientes (Argentina)
Giorgio Giraffa
Affiliation:
Agriculture Research Council, Research Centre for Forage and Dairy Productions, Lodi, Italy
Domenico Carminati
Affiliation:
Agriculture Research Council, Research Centre for Forage and Dairy Productions, Lodi, Italy
Mariángeles Briggiler Marcó
Affiliation:
Instituto de Lactología Industrial, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina
Jorge Reinheimer
Affiliation:
Instituto de Lactología Industrial, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina
Viviana Suárez*
Affiliation:
Instituto de Lactología Industrial, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina
*
*For correspondence; e-mail: vivisuar@fiq.unl.edu.ar
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Abstract

Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 strains as potential host strains, differed only in the phage reactivity against one of them. The phages showed latent periods of 30 min, burst periods of 80±10 min and burst size values of 11·0±1·0 PFU per infected cell as mean value. Identical DNA restriction patterns were obtained for both phages with PvuI, SalI, HindIII and MluI. The viral DNA apparently did not present extremes cos and the structural protein patterns presented four major bands (32·9, 35·7, 43·0 and 66·2 kDa). This study reports the first isolation of bacteriophages of Lb. plantarum from Kefir grains and adds further knowledge regarding the complex microbial community of this fermented milk.

Keywords

bacteriophagesLactobacillus plantarumKefir
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 1 , February 2010 , pp. 7 - 12
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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