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Radioimmunoassay for measurement of bovine α-lactalbumin in serum, milk and tissue culture media

Published online by Cambridge University Press:  01 June 2009

R. Michael Akers ,
Thomas B. McFadden ,
W. E. Beal ,
Albert J. Guidry  and
Harold M. Farrell
R. Michael Akers
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Thomas B. McFadden
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
W. E. Beal
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Albert J. Guidry
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Harold M. Farrell
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
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Summary

A sensitive, specific radioimmunoassay for bovine α-lactalbumin (α-la) suitable for measurement of serum, tissue culture media and milk was developed. α-La was not detected in serum from prepubertal male or female cattle, but was detected as early as 60 d of gestation in nulliparous Holstein heifers, the level being greatly increased during the last one third of gestation. Serum from cross bred beef heifers contained less α-la and it was not detected until late gestation. Concentrations of α-la in serum samples from pregnant multiparous Holstein cows decreased at drying-off and subsequently increased just before parturition. Secretion of α-la by mammary tissue explants from steroid-primed prepubertal Holstein heifers was induced by the addition of bovine prolactin, ovine prolactin or human growth hormone to tissue culture media.

Type
Original Articles
Information
Journal of Dairy Research , Volume 53 , Issue 3 , August 1986 , pp. 419 - 429
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

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References

REFERENCES

Akers, R. M. 1985 Lactogenic hormones: binding sites, mammary growth, secretory cell differentiation and ilk biosynthesis in ruminants. Journal of Dairy Science 68 501–519CrossRefGoogle Scholar
Akers, R. M., Bauman, D. E., Capuco, A. V., Goodman, G. T. & Tucker, H. A. 1981 a Prolactin regulation f milk secretion and biochemical differentiation of mammary epithelial cells in periparturient cows. Endocrinology 109 23–30CrossRefGoogle Scholar
Akers, R. M., Bauman, D. E., Goodman, G. T., Capuco, A. V. & Tucker, H. A. 1981 b Prolactin regulation of cytological differentiation of mammary epithelial cells in periparturient cows. Endocrinology 109 31–40CrossRefGoogle ScholarPubMed
Akers, R. M. & Keys, J. E. 1984 Characterization of lactogenic hormone binding to membranes from ovine nd bovine mammary gland and liver. Journal of Dairy Science 67 2224–2235CrossRefGoogle Scholar
Akers, R. M. & Keys, J. E. 1985 Effects of suckling intensity on human growth hormone binding, biochemical omposition and histological characteristics of ovine mammary glands. Domestic Animal Endocrinology 2 59–172Google Scholar
Akers, R. M. & Lefcourt, A. M. 1983 Teat stimulation-induced prolactin release in non-pregnant and regnant Holstein heifers. Journal of Endocrinology 96 433–442CrossRefGoogle Scholar
Aschaffenburg, R. & Drewry, J. 1957 Improved method for the preparation of crystalline β-lactoglobulin nd α-laetalbumin from cow's milk. Biochemical Journal 65 273–277Google Scholar
Basch, J. J., Douglas, F. W., Procino, L. G., Holsinger, V. H. & Farrell, H. M. 1985 Quantitation of aseins and whey proteins of processed milks and whey protein concentrates, application of gel electrophoresis. Journal of Dairy Science 68 23–31CrossRefGoogle Scholar
Beck, N. F. G. & Tucker, H. A. 1977 Relationships between radioimmunoassays of alpha lactalbumin and rolactin in bovine skim milk. Journal of Dairy Science 60 542–545CrossRefGoogle Scholar
Bolt, D. J. 1981 Development of a homologous radioimmunoassay for ovine follicle stimulating hormone: tudies after estrus, ovariectomy, estradiol and releasing hormone. Journal of Animal Science 53 730–741CrossRefGoogle Scholar
Fleet, I. R., Goode, J. A., Hamon, M. H., Laurie, M. S., Linzell, J. L. & Peaker, M. 1975 Secretory ctivity of goat mammary glands during pregnancy and the onset of lactation. Journal of Physiology 51 763–773Google Scholar
Forsyth, I. A. 1983 The endocrinology of lactation. In Biochemistry of Lactation pp. 309–349 (Ed. Mepham, T. B.) Amsterdam: Elsevier Science PublishersGoogle Scholar
Forsyth, I. A., Byatt, J. C. & Iley, S. 1985 Hormone concentrations, mammary development and milk yield n goats given long-term bromocriptine treatment in pregnancy. Journal of Endocrinology 104 77–85Google Scholar
Gertler, A., Ashkenazi, A. & Madar, Z. 1984 Binding sites of human growth hormone and ovine and bovine rolactins in the mammary gland and the liver of lactating dairy cow. Molecular and Cellular Endocrinology 34 51–57CrossRefGoogle ScholarPubMed
Goodman, G. T., Akers, R. M., Friderici, K. H. & Tucker, H. A. 1983 Hormonal regulation of a-lactalbumin ecretion from bovine mammary tissue cultured in vitro. Endocrinology 112 1324–1330CrossRefGoogle Scholar
Hartmann, P. E. 1973 Changes in the composition and yield of the mammary secretion of cows during the nitiation of lactation. Journal of Endocrinology 59 231–247Google Scholar
Hartmann, P. E., Whitely, J. L. & Willcox, D. L. 1984 Lactose in plasma during lactogenesis, established actation and weaning in sows. Journal of Physiology 347 453–463CrossRefGoogle ScholarPubMed
Kilshaw, P. J. & Slade, H. 1981 Milk protein immune complexes in the cow and calf. Journal of Reproductive Immunology 3 227–236CrossRefGoogle ScholarPubMed
Kuhn, N. J., Carrick, D. J. & Wilde, C. J. 1980 Lactose synthesis: the possibilities of regulation. Journal of Dairy Science 63 328–336Google Scholar
Kuhn, N. J. & Linzell, J. L. 1970 Measurement of the quantity of lactose passing into mammary venous lasma and lymph in goats and in a cow. Journal of Dairy Research 37 203–208CrossRefGoogle Scholar
Linzell, J. L. & Peaker, M. 1974 Changes in colostrum composition and in the permeability of the mammary pithelium at about the time of parturition in the goat. Journal of Physiology 243 129–151CrossRefGoogle Scholar
Martin, R. A., Glass, M. R., Chapman, C., Wilson, G. D. & Woods, K. L. 1980 Human α-lactalbumin and ormonal factors in pregnancy and lactation. Clinical Endocrinology 13 223–230Google Scholar
McFadden, T. B., Akers, R. M. & Beal, W. E. 1984 Response of prepubertal beef and dairy bulls to steroid retreatment followed by in vitro culture of mammary tissue. Journal of Dairy Science 67 (Suppl 1) 173Google Scholar
Morgan, G. & Wooding, F. B. P. 1982 A freeze-fracture study of tight junction structure in sheep mammary land epithelium during pregnancy and lactation. Journal of Dairy Research 49 1–11Google Scholar
Nickerson, S. C. & Akers, R. M. 1984 Biochemical and ultrastructural aspects of milk synthesis and secretion. International Journal of Biochemistry 16 855–865CrossRefGoogle ScholarPubMed
Peaker, M. 1977 Mechanism of milk secretion: milk composition in relation to potential difference across the ammary epithelium. Journal of Physiology 270 489–505Google Scholar
Rodbard, D. 1973 Mathematics of hormone-receptor interaction. I. Basic principles. In Receptors for eproductive Hormones (Workshop conference 1972 Nashville, TE) 289–326 Eds O'Malley, B. W. and eans, A. R.. New York: Plenum Press (Advances in Experimental Medicine and Biology 36)Google Scholar
Smith, K. L. & Schanbaoher, F. L. 1973 Hormone induced lactation in the bovine. I. Lactational erformance following injections of 17 β-estradiol and progesterone. Journal of Dairy Science 56 738–743CrossRefGoogle Scholar
Tucker, H. A. 1981 Physiological control of mammary growth, lactogenesis, and lactation. Journal of Dairy cience 64 1403–1421.Google Scholar
Wheelock, J. V. & Rook, J. A. F. 1966 Lactose in the blood and urine of cows. Journal of Dairy Research 33 37–42CrossRefGoogle Scholar