Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-19T16:53:35.138Z Has data issue: false hasContentIssue false

Studies on the heat stability of milk: I. Behaviour of divalent cations and phosphate in milks heated in a stainless steel system

Published online by Cambridge University Press:  01 June 2009

Douglas G. Dalgleish
Affiliation:
Hannah Research Institute, Ayr, KA6 5HL, UK
Yves Pouliot
Affiliation:
Sciences et Technologie des Aliments, Université Laval, Québec, Canada
Paul Paquin
Affiliation:
Sciences et Technologie des Aliments, Université Laval, Québec, Canada

Summary

Skim milk was heated rapidly to 130 °C in stainless steel tubing, and was then held at this temperature for periods of up to 1 h in a stainless steel holding vessel. Samples taken at intervals during the holding period were analysed for cations, inorganic and organic phosphate and protein in the total sample and in the supernatant after centrifugation at 60 500g. The cation and total phosphate (organic+inorganic) contents of the sedimentable material remained constant throughout the heating, although the caseins became extensively dephosphorylated. Dephosphorylated protein dissociated from the casein micelles during the first 20 min of heating, after which its concentration in the serum began to decrease, perhaps indicating the onset of the heat coagulation reaction.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allen, R. J. L. 1940 The estimation of phosphorus. Biochemical Journal 34 858865CrossRefGoogle ScholarPubMed
Aoki, T. & Kako, Y. 1983 Relation between micelle size and formation of soluble casein on heating concentrated milk. Journal of Dairy Research 50 207213CrossRefGoogle Scholar
Belec, J. & Jenness, R. 1962 Dephosphorylation of casein by heat treatment. II. In skim milks. Journal of Dairy Science 45 2026CrossRefGoogle Scholar
Creamer, L. K., Berry, G. P. & Matheson, A. R. 1978 The effect of pH on protein aggregation in heated skim milk. New Zealand Journal of Dairy Science and Technology 13 915Google Scholar
Dalgleish, D. G., Pouliot, Y. & Paquin, P. 1987 Studies on the heat stability of milk. II. Association and dissociation of particles and the effect of added urea. Journal of Dairy Research 54 3949CrossRefGoogle Scholar
Darling, D. F. 1980 Heat stability of milk. Journal of Dairy Research 47 199210Google Scholar
Evenhuis, N. & ThDevries, R. 1956 a The condition of calcium phosphate in milk. III. Netherlands Milk and Dairy Journal 10 101113Google Scholar
ThEvenhuis, N. & Devries, R. 1956 b The condition of calcium phosphate in milk. IV. Netherlands Milk and Dairy Journal 10 180189Google Scholar
Fox, K. K., Harper, M. K., Holsinger, V. H. & Pallansch, M. J. 1967 Effects of high-heat treatment on stability of calcium caseinate aggregates in milk. Journal of Dairy Science 50 443450CrossRefGoogle Scholar
Fox, P. F. 1982 Heat-induced coagulation of milk. In Developments in Dairy Chemistry–1. Proteins pp, 189228 (Ed. Fox, P. F.) London: Applied Science PublishersGoogle Scholar
Fox, P. F. & Hearn, C. M. 1978 Heat stability of milk: influence of dilution and dialysis against water. Journal of Dairy Research 45 149157Google Scholar
Fox, P. F. & Hoynes, M. C. T. 1975 Heat stability of milk: influence of colloidal calcium phosphate and β-lactoglobulin. Journal of Dairy Research 42 427435Google Scholar
Fox, P. F. & Morrissey, P. A. 1977 Reviews of the progress of Dairy Science: the heat stability of milk. Journal of Dairy Research 44 627646CrossRefGoogle Scholar
Holt, C. 1985 The milk salts: their secretion, concentrations and physical chemistry. In Developments in Dairy Chemistry–3. Lactose and Minor Constituents pp. 143181 (Ed. Fox, P. F.) London: Applied Science Publishers.Google Scholar
Holt, C., Hasnain, S. S. & Hukins, D. W. L. 1982 Structure of bovine milk calcium phosphate determined by X-ray absorption spectroscopy. Biochimica et Biophysica Acta 719 299303Google Scholar
Kannan, A. & Jenness, R. 1961 Relation of milk serum proteins and milk salts to the effects of heat treatment and rennet clotting. Journal of Dairy Science 44 808822CrossRefGoogle Scholar
Kudo, S. 1980 The heat stability of milk: formation of soluble proteins and protein-depleted micelles at elevated temperatures. New Zealand Journal of Dairy Science and Technology 15 255263Google Scholar
Morr, C. V. 1975 Chemistry of milk proteins during food processing. Journal of Dairy Science 58 977984Google Scholar
Morrissey, P. A. 1969 The heat stability of milk as affected by variations in pH and milk salts. Journal of Dairy Research 36 343351CrossRefGoogle Scholar
Pyne, G. T. & McHenry, K. A. 1955 The heat coagulation of milk. Journal of Dairy Research 22 6068CrossRefGoogle Scholar
Sweetsur, A. W. M. & White, J. C. D. 1974 Studies on the heat stability of milk protein. I. Interconversion of type A and type B milk heat-stability curves. Journal of Dairy Research 41 349358Google Scholar
Sweetsur, A. W. M. & White, J. C. D. 1975 Studies on the heat stability of milk protein. III. Effect of heat-induced acidity in milk. Journal of Dairy Research 42 7388Google Scholar