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Digit ratio (2D:4D) and amniotic testosterone and estradiol: an attempted replication of Lutchmaya et al. (2004)

Published online by Cambridge University Press:  21 January 2021

Gareth Richards Open the ORCID record for Gareth Richards [Opens in a new window] ,
Wendy V. Browne  and
Mihaela Constantinescu
Gareth Richards*
Affiliation:
School of Psychology, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK Autism Research Centre, Department of Psychiatry, University of Cambridge, Cambridge, UK
Wendy V. Browne
Affiliation:
Faculty of Education, University of Cambridge, Cambridge, UK
Mihaela Constantinescu
Affiliation:
Gender Development Research Centre, Department of Psychology, University of Cambridge, Cambridge, UK School of Psychology, University of East London, London, UK
*
Address for correspondence: Gareth Richards, School of Psychology, Faculty of Medical Sciences, Newcastle University, 2.27 Ridley Building 1, Queen Victoria Road, Newcastle upon Tyne, UK. Email: gareth.richards@ncl.ac.uk
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Abstract

The ratio of length between the second (index) and fourth (ring) fingers (digit ratio or 2D:4D) is frequently employed as a retrospective marker of prenatal sex hormone exposure. Lutchmaya et al. (2004) reported that the ratio of testosterone (T) to estradiol (E) present in second-trimester amniotic fluid was negatively correlated with digit ratios for the right hand (but not the left hand) in a sample of 29 children at 2-year follow-up. This observation is frequently cited as evidence for the measure’s validity but has not been replicated. We therefore present the findings of another study of amniotic T and E that did not find evidence for these effects at 4½-year follow-up. The confidence intervals were large, the direction of correlations observed was generally erratic, and the overall findings question the premise that second-trimester sex hormones affect the development of digit length ratios in humans.

Keywords

2D:4Ddigit ratioestradiolprenatal sex hormonestestosterone
Type
Brief Reports
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 6 , December 2021 , pp. 859 - 864
Copyright
© The Author(s), 2021. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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References

Manning, JT, Scutt, D, Wilson, J, Lewis-Jones, DI. The ratio of 2nd to 4th digit length: a predictor of sperm numbers and concentrations of testosterone, luteinizing hormone and oestrogen. Hum Reprod. 1998; 13, 3000–3004. https://doi.org/10.1093/humrep/13.11.3000.CrossRefGoogle ScholarPubMed
Leslie, M. The mismeasure of hands? Science. 2019; 364, 923–926. https://doi.org/10.1126/science.364.6444.923.CrossRefGoogle Scholar
Richards, G. What is the evidence for a link between digit ratio (2D:4D) and direct measures of prenatal sex hormones? Early Hum Dev. 2017; 113, 71–72. https://doi.org/10.1016/j.earlhumdev.2017.08.003 CrossRefGoogle ScholarPubMed
Richards, G, Gomes, MC, Ventura, T. Testosterone measured from amniotic fluid and maternal plasma shows no significant association with directional asymmetry in newborn digit ratio (2D:4D). J Dev Orig Health Dis. 2019; 10, 362–367. https://doi.org/10.1017/S2040174418000752 CrossRefGoogle Scholar
Richards, G, Browne, WV, Aydin, E, et al. Digit ratio (2D:4D) and congenital adrenal hyperplasia (CAH): systematic literature review and meta-analysis. Horm Behav. 2020; 126, 104867. https://doi.org/10.1016/j.yhbeh.2020.104867.CrossRefGoogle ScholarPubMed
Berenbaum, SA, Bryk, KK, Nowak, N, Quigley, CA, Moffat, S. Fingers as a marker of prenatal androgen exposure. Endocrinology. 2009; 150, 5119–5124. https://doi.org/10.1210/en.2009–0774 CrossRefGoogle ScholarPubMed
van Hemmen, J, Cohen-Kettenis, PT, Steensma, TD, Veltman, DJ, Bakker, J. Do sex differences in CEOAEs and 2D:4D ratios reflect androgen exposure? A study in women with complete androgen insensitivity syndrome. Biol Sex Differ. 2017; 8, 11. https://doi.org/10.1186/s13293–017–0132-z CrossRefGoogle ScholarPubMed
Medland, SE, Loehlin, JC, Martin, NG. No effects of prenatal hormone transfer on digit ratio in a large sample of same- and opposite-sex dizygotic twins. Pers Individ Differ. 2008; 44, 1225–1234. https://doi.org/10.1016/j.paid.2007.11.017 CrossRefGoogle Scholar
van Anders, SM, Vernon, PA, Wilbur, CJ. Finger-Length ratios show evidence of prenatal hormone-transfer between opposite-sex twins. Horm Behav. 2006; 49, 315–319. https://doi.org/10.1016/j.yhbeh.2005.08.003 CrossRefGoogle ScholarPubMed
Voracek, M, Dressler, SG. Digit ratio (2D:4D) in twins: heritability estimates and evidence for a masculinized trait expression in women from opposite-sex pairs. Psychol Rep. 2007; 100, 115–126. https://doi.org/10.2466/PR0.100.1.115–126 CrossRefGoogle ScholarPubMed
Manning, JT. Digit Ratio: A Pointer to Fertility, Behavior, and Health, 2002. Rutgers University Press, New Brunswick, NJ.Google Scholar
Richards, G, Bellin, W, Davies, W. Familial digit ratio (2D:4D) associations in a general population sample from Wales. Early Hum Dev. 2017; 112, 14–19. https://doi.org/10.1016/j.earlhumdev.2017.06.006 CrossRefGoogle Scholar
Voracek, M, Dressler, SG. Brief communication: familial resemblance in digit ratio (2D:4D). Am J Phys Anthrop. 2009; 140, 376–380. https://doi.org/10.1002/ajpa.21105 CrossRefGoogle Scholar
Lippa, RA. Digit Ratio: A Pointer to Fertility, Behavior, and Health. By John T. Manning, 2002. Rutgers University Press, New Brunswick, New Jersey, 173 pp., $27.00 (paper), $65.00 (hardcover). Reviewed by Richard A. Lippa, Ph.D. Arch Sex Behav. 2003; 32, 587–590.Google Scholar
Lutchmaya, S, Baron-Cohen, S, Raggatt, P, Knickmeyer, RC, Manning, JT. 2nd to 4th digit ratios, fetal testosterone and estradiol. Early Hum Dev. 2004; 77, 23–28. https://doi.org/10.1016/j.earlhumdev.2003.12.002.CrossRefGoogle ScholarPubMed
Ventura, T, Gomes, MC, Pita, A, Neto, MT, Taylor, A. Digit ratio (2D:4D) in newborns: influences of prenatal testosterone and maternal environment. Early Hum Dev. 2013; 89, 107–112. https://doi.org/10.1016/j.earlhumdev.2012.08.009.CrossRefGoogle ScholarPubMed
Bergman, K, Glover, V, Sarkar, P, Abbott, DH, O’Connor, TG. In utero cortisol and testosterone exposure and fear reactivity in infancy. Horm Behav. 2010; 57, 306–312. https://doi.org/10.1016/j.yhbeh.2009.12.012.CrossRefGoogle ScholarPubMed
Constantinescu, M. Are finger ratios a useful measure of androgenic influences on sexual differentiation? (Unpublished MPhil Thesis). 2009. University of Cambridge.Google Scholar
Manning, JT, Fink, B. Are there any “direct” human studies of digit ratio (2D:4D) and measures of prenatal sex hormones? Early Hum Dev. 2017; 113, 73–74. https://doi.org/10.1016/j.earlhumdev.2017.09.003.CrossRefGoogle ScholarPubMed
Malas, MA, Dogan, S, Evcil, EH, Desdicioglu, K. Fetal development of the hand, digits and digit ratio (2D : 4D). Early Hum Dev. 2006; 82, 469–475. https://doi.org/10.1016/j.earlhumdev.2005.12.002.CrossRefGoogle Scholar
Knickmeyer, RC, Woolson, S, Hamer, RM, Konneker, T, Gilmore, JH. 2D:4D ratios in the first 2 years of life: stability and relation to testosterone exposure and sensitivity. Horm Behav. 2011; 60, 256–263. https://doi.org/10.1016/j.yhbeh.2011.05.009.CrossRefGoogle ScholarPubMed
Trivers, R, Manning, JT, Jacobson, A. A longitudinal study of digit ratio (2D:4D) and other finger ratios in Jamaican children. Horm Behav. 2006; 49, 150–156. https://doi.org/10.1016/j.yhbeh.2005.05.023.CrossRefGoogle ScholarPubMed
Rodeck, CH, Gill, D, Rosenberg, DA, Collins, WP. Testosterone levels in midtrimester maternal and fetal plasma and amniotic fluid. Prenat Diagn. 1985; 5, 175–181. https://doi.org/10.1002/pd.1970050303.CrossRefGoogle ScholarPubMed
Hickey, M, Doherty, DA, Hart, R, et al. Maternal and umbilical cord androgen concentrations do not predict digit ratio (2D:4D) in girls: a prospective cohort study. Psychoneuroendocrinology. 2010; 35, 1235–1244. https://doi.org/10.1016/j.psyneuen.2010.02.013.CrossRefGoogle Scholar
Hollier, LP, Keelan, JA, Jamnadass, ESL, et al. Adult digit ratio (2D:4D) is not related to umbilical cord androgen or estrogen concentrations, their ratios or net bioactivity. Early Hum Dev. 2015; 91, 111–117. https://doi.org/10.1016/j.earlhumdev.2014.12.011.CrossRefGoogle ScholarPubMed
Voracek, M, Manning, JT, Dressler, SG. Repeatability and interobserver error of digit ratio (2D:4D) measurements made by experts. Am J Hum Biol. 2007; 19, 142–146. https://doi.org/10.1002/ajhb.20581.CrossRefGoogle ScholarPubMed
Ribeiro, E, Neave, N, Morais, RN, Manning, JT. Direct versus indirect measurement of digit ratio (2D:4D): a critical review of the literature and new data. Evol Psychol. 2016; 14, 1–8. https://doi.org/10.1177/1474704916632536.CrossRefGoogle Scholar
Barona, M, Kothari, R, Skuse, D, et al. Social communication and emotion difficulties and second to fourth digit ratio in a large community-based sample. Mol Autism. 2015; 6, 68. https://doi.org/10.1186/s13229–015–0063–7.CrossRefGoogle Scholar