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Programmed regulation of rat offspring adipogenic transcription factor (PPARγ) by maternal nutrition*

Published online by Cambridge University Press:  19 August 2015

M. Desai ,
J. K. Jellyman ,
G. Han ,
R. H. Lane  and
M. G. Ross
M. Desai*
Affiliation:
Perinatal Research Laboratories, Department of Obstetrics and Gynecology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA
J. K. Jellyman
Affiliation:
Perinatal Research Laboratories, Department of Obstetrics and Gynecology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA
G. Han
Affiliation:
Perinatal Research Laboratories, Department of Obstetrics and Gynecology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA
R. H. Lane
Affiliation:
Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA
M. G. Ross
Affiliation:
Perinatal Research Laboratories, Department of Obstetrics and Gynecology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA
*
*Address for correspondence: M. Desai, PhD, Department of Obstetrics and Gynecology, LABioMed at Harbor-UCLA Medical Center, 1124 West Carson Street Box 446, RB-1 Bldg., Torrance, CA 90502, USA. (Email mdesai@obgyn.humc.edu)
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Abstract

We determined the protein expression of adipogenic transcription factor, peroxisome proliferator-activated receptor gamma (PPARγ) and its co-repressor and co-activator complexes in adipose tissue from the obese offspring of under- and over-nourished dams. Female rats were fed either a high-fat (60% kcal) or control (10% kcal) diet before mating, and throughout pregnancy and lactation (Mat-OB). Additional dams were 50% food-restricted from pregnancy day 10 to term [intrauterine growth-restricted (IUGR)]. Adipose tissue protein expression was analyzed in newborn and adult male offspring. Normal birth weight Mat-OB and low birth weight IUGR newborns had upregulated PPARγ with variable changes in co-repressors and co-activators. As obese adults, Mat-OB and IUGR offspring had increased PPARγ with decreased co-repressor and increased co-activator expression. Nutritionally programmed increased PPARγ expression is associated with altered expression of its co-regulators in the newborn and adult offspring. Functional studies of PPARγ co-regulators are necessary to establish their role in PPARγ-mediated programmed obesity.

Keywords

co-activator proteinsco-repressor proteinsmaternal obesitymaternal undernutritionperoxisome proliferator-activated receptors
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 6 , Issue 6 , December 2015 , pp. 530 - 538
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2015 

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Footnotes

*

The work was done at the Perinatal Research Laboratories, Department of Obstetrics and Gynecology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA.

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