The effects of passive scalar anisotropy on subgrid-scale (SGS) physics and modelling for large-eddy simulations are studied experimentally. Measurements are performed across a moderate Reynolds number wake flow generated by a heated cylinder, using an array of four X-wire and four cold-wire probes. By varying the separation distance among probes in the array, we obtain filtered and subgrid quantities at three different filter sizes. We compute several terms that comprise the subgrid dissipation tensor of kinetic energy and scalar variance and test for isotropic behaviour, as a function of filter scale. We find that whereas the kinetic energy dissipation tensor tends towards isotropy at small scales, the SGS scalar-variance dissipation remains anisotropic independent of filter scale. The eddy-diffusion model predicts isotropic behaviour, whereas the nonlinear (or tensor eddy diffusivity) model reproduces the correct trends, but overestimates the level of scalar dissipation anisotropy. These results provide some support for so-called mixed models but raise new questions about the causes of the observed anisotropy.