Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-28T23:37:09.475Z Has data issue: false hasContentIssue false
  • English
  • Français

The ability of the nematode-trapping fungus Duddingtonia flagrans to reduce the transmission of

Published online by Cambridge University Press:  11 April 2024

A.S. Fernández ,
M. Larsen ,
P. Nansen ,
E. Henningsen ,
J. Grønvold ,
J. Wolstrup ,
S.A. Henriksen  and
H. Bjørn
A.S. Fernández*
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark: Área de Parasitología y Enfermedades Parasitarias, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Pje. Arroyo Seco s/n, 7000,- Tandil, Argentina
M. Larsen
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark:
P. Nansen
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark:
E. Henningsen
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark:
J. Grønvold
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark: Section of Zoology
J. Wolstrup
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark: Section of Microbiology, Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871, Frederiksberg C, Denmark
S.A. Henriksen
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark: Danish Veterinary Laboratory, 27 Bülowsvej, DK-1790, Copenhagen V, Denmark
H. Bjørn
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, 3 Ridebanevej, DK-1870, Frederiksberg C, Denmark: Danish Veterinary Laboratory, 27 Bülowsvej, DK-1790, Copenhagen V, Denmark
*
*Fax: +45 3528 2774E-mail: asf@kvl.dk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The ability of two isolates of the nematode-trapping fungus Duddingtonia flagrans to reduce the numbers of gastrointestinal nematode larvae on herbage was tested in three plot studies. Artificially prepared cow pats containing Ostertagia ostertagi eggs, with and without fungal spores, were deposited on pasture plots two or three times during the grazing season in 1995, 1996 and 1997. The herbage around each pat was sampled fortnightly over a period of 2 months and the number of infective larvae was recorded. At the end of the sampling period, the remainder of the faecal pats was collected to determine the wet weight, dry weight, and content of organic matter. The infective larvae remaining in the pats were extracted. Faecal cultures showed that both fungal isolates significantly reduced the number of infective larvae. Significantly fewer larvae were recovered from herbage surrounding fungus-treated pats compared with control pats in all three experiments, reflecting the ability of the fungus to destroy free-living larval stages in the faecal pat environment. After 8 weeks on pasture there were no differences between control and fungus-treated pats with respect to wet weight, dry weight, and organic matter content. This indicates that the degradation of faeces was not negatively affected by the presence of the fungus.

Type
Review Article
Information
Journal of Helminthology , Volume 73 , Issue 2 , February 1999 , pp. 115 - 122
Copyright
Copyright © Cambridge University Press 1999

References

Cooke, R.C. (1969) Two nematode trapping Hyphomycetes, Duddingtonia flagrans gen. et comb. nov. and Monacrosporium mutabilis sp. nov. Transactions of the British Mycological Society 53, 315–319.CrossRefGoogle Scholar
Durie, P.H. (1961) Parasitic gastroenteritis of cattle: the distribution and survival of infective strongyle larvae on pasture. Australian Journal of Agricultural Research 12, 1200–1211.CrossRefGoogle Scholar
Fernández, A.S., Larsen, M., Nansen, P., Grønvold, J., Henriksen, S.A. & Wolstrup, J. (1997) Effect of the nematode-trapping fungus Duddingtonia flagrans on the free-living stages of horse parasitic nematodes: a plot study. Veterinary Parasitology 73, 257–266.CrossRefGoogle Scholar
Grønvold, J. (1984) Isolation of infective Cooperia spp. larvae from soil samples. Acta Veterinaria Scandinavica 25, 139–142.Google Scholar
Grønvold, J., Wolstrup, J., Larsen, M., Henriksen, S.A. & Nansen, P. (1993) Biological control of Ostertagia ostertagi by feeding selected nematode-trapping fungi to calves. Journal of Helminthology 67, 31–36.CrossRefGoogle ScholarPubMed
Grønvold, J., Nansen, P., Henriksen, S.A., Larsen, M., Wolstrup, J., Bresciani, J., Rawat, H. & Fribert, L. (1996) Induction of traps by Ostertagia ostertagi larvae, chlamydospore production and growth rate in the nematode-trapping fungus Duddingtonia flagrans. Journal of Helminthology 70, 291–297.CrossRefGoogle ScholarPubMed
Henriksen, S.A. & Korsholm, M. (1983) A method for culture and recovery of gastrointestinal strongyle larvae. Nordisk Veterinærmedicin 35, 429–430.Google ScholarPubMed
Jørgensen, R.J. (1975) In vitro migration of Dictyocaulus viviparus larvae: migration of the infective stage in agar gel. International Journal for Parasitology 5, 199–202.CrossRefGoogle Scholar
Larsen, M., Wolstrup, J., Henriksen, S.A., Dackman, C., Grønvold, J. & Nansen, P. (1991) In vitro stress selection of nematode-trapping fungi for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 65, 193–200.CrossRefGoogle Scholar
Larsen, M., Wolstrup, J., Henriksen, S.A., Grønvold, J. & Nansen, P. (1992) In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes. Journal of Helminthology 66, 137–141.CrossRefGoogle ScholarPubMed
Larsen, M., Nansen, P., Wolstrup, J., Grønvold, J., Henriksen, S.A. & Zorn, A. (1995) Biological control of trichostrongyles in calves by the fungus Duddingtonia flagrans fed to animals under natural grazing conditions. Veterinary Parasitology 60, 321–330.CrossRefGoogle ScholarPubMed
Mwegoha, W.H. & Jørgensen, R.J. (1977) Recovery of infective 3rd stage larvae of Haemonchus contortus and Ostertagia ostertagi by migration in agar gel. Acta Veterinaria Scandinavica 18, 293–299.CrossRefGoogle ScholarPubMed
Nansen, P., Larsen, M., Grønvold, J., Wolstrup, J., Zorn, A. & Henriksen, S.A. (1995) Prevention of clinical trichostrongylidosis in calves by strategic feeding with the predacious fungus Duddingtonia flagrans. Parasitology Research, 81, 371–374.CrossRefGoogle ScholarPubMed
Roepstorff, A. & Nansen, P. (1998) The epidemiology, diagnosis and control of helminth parasites of swine. A methodological guideline. FAO, Rome, Italy. (In press).Google Scholar
Rose, J.H. (1962) Further observations on the free-living stages of Ostertagia ostertagi in cattle. Journal of Comparative Pathology and Therapeutics 72, 11–18.CrossRefGoogle ScholarPubMed
Siegel, S. (1956) Non-parametric statistics for the behavioural sciences. 312 pp. Kogakusha, Tokyo, McGraw-Hill.Google Scholar
Sommer, C., Steffansen, B., Overgaard Nielsen, B., Grønvold, J., Vagn Jensen, K.-M., Brochner Jespersen, J., Springborg, J. & Nansen, P. (1992) Ivermectin excreted in cattle dung after subcutaneous or pour-on treatment: concentrations and impact on dung fauna. Bulletin of Entomological Research 82, 257–264.CrossRefGoogle Scholar
Wolstrup, J., Grønvold, J., Henriksen, S.A., Nansen, P., Larsen, M., Bøgh, H.O. & Ilsøe, B. (1994) An attempt to implement the nematode-trapping fungus Duddingtonia flagrans in biological control of trichostrongyle infections of first year grazing calves. Journal of Helminthology 68, 175–180.CrossRefGoogle ScholarPubMed