Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-23T04:37:28.111Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of polyunsaturated fatty acid composition of Strongyloides ratti in relation to development

Published online by Cambridge University Press:  05 June 2009

Takeya Minematsu ,
Seietsu Yamazaki ,
Yoshinori Uji ,
Hiroaki Okabe ,
Masataka Korenaga  and
Isao Tada
Takeya Minematsu
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Seietsu Yamazaki
Affiliation:
Analytical Chemistry Group, Tsukuba Research Center, Mitsubishi Petrochemical Co., Ltd. 8-3-1, Chuo, Ami-machi, Inashikigun, Ibaraki 300-03, Japan
Yoshinori Uji
Affiliation:
Laboratory Medicine, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Hiroaki Okabe
Affiliation:
Laboratory Medicine, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Masataka Korenaga
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Isao Tada
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

The effect of linolenic acid (C18:3ω3) on the development of Strongyloides ratti first-stage larvae (L1) in culture was studied. The fatty acid composition of S. ratti free-living generations was analyzed by gas chromatography. L1 had abundant linoleic acid (C18:2ω6) but its proportion decreased with development. On the contrary, eicosapentaenoic acid (C20:5ω3) and C20:4ω3 were prominent in the filariform larva (L3). Because C20:5ω3 is generally synthesized from C18:3ω3 via C20:4ω3, the high ratio of C20:5ω3/C18:3ω3 of L3 in all the free-living generations suggested that polyunsaturated fatty acid metabolism, particularly the ω-3 series, and eicosanoids produced had important roles in the development of S. ratti L1.

Keywords

Strongyloides rattifatty acidslinolenic acidlinoleic acideicosapentaenoic acideicosanoidsdevelopment
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 4 , December 1990 , pp. 303 - 309
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barrett, J. (1969) The effect of ageing on the metabolism of the infective larvae of Strongyloides ratti Sandground, 1925. Parasitology, 59, 3–17.CrossRefGoogle Scholar
Folch, L., Lee, M. & Stanley, G. H. S. (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226, 497–509.CrossRefGoogle ScholarPubMed
Frayha, G. J. & Smyth, J. D. (1983) Lipid metabolism in parasitic helminths. Advances in Parasitology, 22, 309–387.CrossRefGoogle ScholarPubMed
Fusco, A. C., Salafsky, B. & Kevin, M. B. (1985) Schistosoma mansoni: Eicosanoid production by cercariae. Experimental Parasitology, 59, 44–50.CrossRefGoogle ScholarPubMed
Fusco, A. C., Salafsky, B. & Delbrook, K. (1986) Schistosoma mansoni: Production of cercarial eicosanoids as correlates of penetration and transformation. Journal of Parasitology, 72, 397–404.CrossRefGoogle ScholarPubMed
Fusco, A. C., Salafsky, B., Whitely, K. & Yohe, S. (1987) Schistosoma mansoni: pH dependence of cercarial eicosanoid production, penetration, and transformation. Experimental Parasitology, 64, 139–146.CrossRefGoogle ScholarPubMed
Jacobsen, N. S. & Fairbairn, D. (1967) Lipid metabolism in helminth parasites. III. Biosynthesis and interconversion of fatty acids by Hymenolepis diminuta (Cestoda). Journal of Parasitology, 53, 355–361.CrossRefGoogle Scholar
Leid, R. W. & Mcconnell, L. A. (1983a) PGE2 generation and release by the larval stage of the cestode, Taenia taeniaeformis. Prostaglandins, Leukotrienes and Medicine, 11, 317–323.CrossRefGoogle ScholarPubMed
Leid, R. W. & Mcconnell, L. A. (1983b) Thromboxane A2 generation by the larval cestode, Taenia taeniaeformis. Clinical Immunology and Immunopathology, 28, 67–76.CrossRefGoogle ScholarPubMed
Liu, L. X. & Weller, P. F. (1989) Brugia malayi: Microfilarial polyunsaturated fatty acid composition and synthesis. Experimental Parasitology, 69, 198–203.CrossRefGoogle ScholarPubMed
Metcalfe, L. D. & Schmitz, A. A. (1961) The rapid preparation of fatty acid esters for gas chromatographic analysis.Analytical Chemistry, 33, 363–364.CrossRefGoogle Scholar
Meyer, F., Kimura, S. & Mueller, J. F. (1966) Lipid metabolism in the larval and adult forms of the tapeworm Spirometra mansonoides. Journal of Biological Chemistry, 241, 4224–4232.CrossRefGoogle ScholarPubMed
Minematsu, T., Mimori, T., Tanaka, M. & Tada, I. (1989) The effect of fatty acids on the developmental direction of Strongyloides ratti first-stage larvae. Journal of Helminthology, 63, 102–106.CrossRefGoogle ScholarPubMed
Salafsky, B., Wang, Y-S., Fusco, A. C. & Antonacci, J. (1984a) The role of essential fatty acids and prostaglandins in cercarial penetration (Schistosoma mansoni). Journal of Parasitology, 70, 656–660.CrossRefGoogle ScholarPubMed
Salafsky, B., Wang, Y-S, Kevin, M. B., Hill, H. & Fusco, A. C. (1984b) The role of prostaglandins in cercarial (Schistosoma mansoni) response to free fatty acids. Journal of Parasitology, 70, 584–591.CrossRefGoogle ScholarPubMed
Salafsky, B., Fusco, A. C., Whitley, K., Nowicki, D. & Ellenberger, B. (1988) Schistosoma mansoni: Analysis of cercarial transformation methods. Experimental Parasitology, 67, 116–127.CrossRefGoogle ScholarPubMed
Tada, I., Mimori, T. & Nakai, M. (1979) Migration route of Strongyloides ratti in albino rats. Japanese Journal of Parasitology, 28, 219–227.Google Scholar
Taguchi, I., Suto, C. & Kumada, N. (1989) Studies on the development and differentiation of Strongyloides ratti. I. Improved isolation method of the first-stage larvae. Japanese Journal of Parasitology, 38, 38–45. (In Japanese with English summary).Google Scholar
Turner, A. C. & Hutchison, W. F. (1979) Lipid synthesis in the adult dog heartworm, Dirofilaria immitis. Comparative Biochemistry and Physiology, 64B, 403–405.Google Scholar