Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-01T21:40:24.030Z Has data issue: false hasContentIssue false
  • English
  • Français

A comparison of Echinostoma trivolvis and E. caproni using random amplified polymorphic DNA analysis

Published online by Cambridge University Press:  05 June 2009

T. Fujino ,
Y. Takahashi  and
B. Fried
T. Fujino
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Y. Takahashi
Affiliation:
Department of Parasitology, School of Medicine, Gifu University, Gifu 500, Japan
B. Fried
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
Get access Rights & Permissions [Opens in a new window]

Abstract

The random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique was applied to two closely-related echinostome species, Echinostoma trivolvis and E. caproni, to demonstrate interspecffic polymorphisms of genomic DNA. Band patterns generated using five individual primers showed that these two echinostomes were genetically distinct, although they share genomic DNA to some extent.

Type
Research Note
Information
Journal of Helminthology , Volume 69 , Issue 3 , September 1995 , pp. 263 - 264
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bowditch, B.M., Albright, D.G., Williams, G.K. & Braun, M.J. (1993) Use of randomly amplified polymorphic DNA markers in comparative genome studies. Methods in Enzymology 224, 294–309.CrossRefGoogle ScholarPubMed
Christensen, N.ø., Fried, B. & Kanev, I. (1990) Taxonomy of 37-collar spined Echinostoma (Trematoda: Echinostomatidae) in studies on the population regulation in experimental rodent hosts. Angewandte Parasitologie 31, 127–130.Google ScholarPubMed
Dias Neto, E., Pereira de Souza, C., Rollinson, D., Katz, N., Pena, S.D.J. & Simpson, A.J.G. (1993) The random amplification of polymorphic DNA allows the identification of strains and species of schistosome. Molecular and Biochemical Parasitology 57, 83–88.CrossRefGoogle ScholarPubMed
Fujino, T. & Fried, B. (1993) Expulsion of Echinostoma trivolvis (Cort, 1914) Kanev, 1985 and retention of E. caproni Richard, 1964 (Trematoda: Echinostomatidae) in C3H mice: pathological, ultrastructural, and cytochemical effects on the host intestine. Parasitology Research 79, 286–292.CrossRefGoogle ScholarPubMed
Huffman, J.E. & Fried, B. (1990) Echinostoma and echinostomiasis. Advances in Parasitology 29, 215–269.CrossRefGoogle ScholarPubMed
Kanev, I. (1985) On the morphology, biology, ecology and taxonomy of E. revolutum group (Trematoda: Echinostomatidae: Echinostoma). PhD Dissertation, University of Sofia, Bulgaria.Google Scholar
Kaukas, A., Neto, E.D., Simpson, A.J.G., Southgate, V.R. & Rollinson, D. (1994) A phylogenetic analysis of Schistosoma haematobium group species based on randomly amplified polymorphic DNA. International Journal for Parasitology 24, 285–290.CrossRefGoogle ScholarPubMed
Kristensen, A.R. & Fried, B. (1991) A comparison of Echinostoma caproni and Echinostoma trivolvis (Trematoda: Echinostomatidae) adults using isoelectrofocusing. Journal of Parasitology 77, 496–498.CrossRefGoogle ScholarPubMed
Ross, G.C., Fried, B. & Southgate, V.R. (1989) Echinostoma revolutum and E. liei: observations on enzymes and pigments. Journal of Natural History 23, 977–981.CrossRefGoogle Scholar
Welsh, J. & McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 7213–7218.CrossRefGoogle ScholarPubMed
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.CrossRefGoogle ScholarPubMed