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Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep

Published online by Cambridge University Press:  27 July 2022

L.H. Olmos Open the ORCID record for L.H. Olmos [Opens in a new window] ,
A. Pantiu ,
A. Avellaneda-Cáceres ,
P.N. Valencia ,
P.N. Cayo ,
M. Signorini  and
J.F. Micheloud
L.H. Olmos*
Affiliation:
Instituto Nacional de Tecnología Agropecuaria (INTA), CIAP, Instituto de Investigación Animal del Chaco Semiárido, Área de Investigación en Salud Animal, EEA Salta, Salta, Argentina Facultad de Ciencias Agrarias y Veterinarias, Universidad Católica de Salta, Salta, Argentina
A. Pantiu
Affiliation:
Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Montecarlo, Misiones, Argentina
A. Avellaneda-Cáceres
Affiliation:
Instituto Nacional de Tecnología Agropecuaria (INTA), CIAP, Instituto de Investigación Animal del Chaco Semiárido, Área de Investigación en Salud Animal, EEA Salta, Salta, Argentina Facultad de Ciencias Agrarias y Veterinarias, Universidad Católica de Salta, Salta, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), EEA Salta, Salta, Argentina
P.N. Valencia
Affiliation:
Instituto San Cayetano N° 8092 Tecnicatura Superior en Laboratorio– Salta Capital, Salta, Argentina
P.N. Cayo
Affiliation:
Instituto San Cayetano N° 8092 Tecnicatura Superior en Laboratorio– Salta Capital, Salta, Argentina
M. Signorini
Affiliation:
Instituto de Investigación de la Cadena Láctea (CONICET – INTA), Ruta 34 Km 227, Rafaela, Santa Fe, Argentina
J.F. Micheloud
Affiliation:
Instituto Nacional de Tecnología Agropecuaria (INTA), CIAP, Instituto de Investigación Animal del Chaco Semiárido, Área de Investigación en Salud Animal, EEA Salta, Salta, Argentina Facultad de Ciencias Agrarias y Veterinarias, Universidad Católica de Salta, Salta, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), EEA Salta, Salta, Argentina
*
Author for correspondence: L.H. Olmos, E-mail: olmos.leandro@inta.gob.ar
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Abstract

Eurytrematosis is a disease caused by flukes of the genus Eurytrema. These parasites infect the pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing eurytrematosis through the analysis of faecal samples can be difficult because most of the available techniques are considered of low sensitivity. In this context, a modification of the Dennis, Stone and Swanson technique (Belem Sedimentation Technique, BST) was previously developed to increase the probability of detecting infected animals; nevertheless, the values of eggs per gram obtained using the modified technique are generally low. We proposed a modification of the this technique (MBST), to increase the sensitivity and detection rate of infected animals. The objective of this work was to describe MBST and compare it with BST. Faecal samples of 212 clinically healthy animals (174 from cattle and 38 from sheep) from 20 farms were taken by the intra-rectal route and stored at 4°C. The samples were processed using BST and MBST. Positive samples amounted to 55 (25.9%) using BST and 121 (57.1%) using MBST. In the simples from cattle, 52 (29.8%) and 107 (61.4%) were positive in BST and MBST, respectively. In sheep, three (7.8%) and 14 (36.8%) positive samples were obtained in BST and MBST, respectively.The results obtained using the two methods were significantly different, indicating a lack of agreement between their findings. The results suggest that MBST is a more sensitive method to detect Eurytrema spp. eggs in faeces than BST.

Keywords

Eurytremadiagnosiscoprologysedimentation
Type
Short Communication
Information
Journal of Helminthology , Volume 96 , 2022 , e53
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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References

Araújo, JV and Belém, PD (1993) Efeito Anti-helmíntico do albendazole sobre a contagem e ovos de Eurytrema sp. (Trematoda) em fezes de bovinos. Arquivo Brasileiro de Medicina Veterinária E Zootecnia 45, 111–114.Google Scholar
Araújo, JV and Belém, PD (1994) Curso natural da eliminação de ovos de Eurytrema sp. Looss, 1907 nas fezes de bezerros. Brazilian Journal of Veterinary Research and Animal Science 31, 125–129.CrossRefGoogle Scholar
Azevedo, JR, Mannigel, RC, Agulhon, AZ, Borba, TR, Barbiéri, AW, de Oliveira, DC, Headley, SA and Janeiro, V (2004) Prevalence and geographical distribution of bovine eurytrematosis in cattle slaughtered in northern Paraná, Brazil. Pesquisa Veterinária Brasileira 24, 23–26. DOI: 10.1590/S0100-736X2004000100006.CrossRefGoogle Scholar
Bassani, AC (2005) Estudo epidemiológico e patológico da euritrematose bovina. Londrina, Tese (Mestrado em Ciência Animal Concentraçâo Sanidade Animal) Universidade Estadual de Londrina.Google Scholar
Bassani, CA, Sangioni, LA, Saut, JPE, Yamamura, MH and Headley, SA (2006) Epidemiology of eurytrematosis (Eurytrema spp. Trematoda: Dicrocoeliidae) in slaughtered beef cattle from the central-west region of the State of Paraná, Brazil. Veterinary Parasitology 141, 356–361.CrossRefGoogle ScholarPubMed
Bassani, CA, Sangioni, LA, Saut, JPE, Headley, SA and Yamamura, MH (2007) Euritrematose bovina. Semina: Ciências Agrárias 28, 299–315. DOI: 10.1016/j.vetpar.2006.06.003.Google Scholar
Belém, PD, Rodrigues de Oliveira, M and Roberto Padovani, C (1992) Adaptation of the Dennis, Stone & Swanson's technique for parasitologic diagnostic of Eurytrema sp. infection in cattle. Brazilian Journal of Veterinary Research and Animal Science 29, 303–307.CrossRefGoogle Scholar
Belém, PAD, Oliveira, MRD, Padovani, CR and Luvizotto, MCR (1994) Alterações pancreáticas em bovinos naturalmente infectados por Eurytrema sp. e sua associação com a carga parasitária e o número de ovos por grama de fezes (OPG). Brazilian Journal of Veterinary Research and Animal Science 31, 273–281.CrossRefGoogle Scholar
Brant, PC (1962) Frequencia de algumas parasitoses em carcacas evísceras de bovinos abatidos em Belo Horizonte. Arquivos da Escola de Veterinaria de Minas Gerais 14, 127–132.Google Scholar
Chinone, S and Itagaki, H (1976) Development of Eurytrema pancreaticum (TREMATODA). II. Development in definitive hosts. Bulletin of the Azabu Veterinary College, Sagamihara 1, 173–181.Google Scholar
Dennis, WR, Stone, WM and Swanson, LE (1954) A new laboratory and field diagnostic test for fluke ova in feces. Journal of the American Veterinary Medical Association 124, 47–50.Google ScholarPubMed
De Sousa, DER, Barbosa, EDF, Wilson, TM, Machado, M, Oliveira, WJ, Duarte, MA and de Castro, MB (2021) Eurytrema coelomaticum natural infection in small ruminants: a neglected condition. Parasitology 148, 576–583.CrossRefGoogle ScholarPubMed
Leite, KG, Lopes-Torres, EJ, Souza, JGR, Neves, RH, Gomes, DC and Machado-Silva, JR (2020) Eurytrema coelomaticum: updated morphology of adult worms using advanced microscopy experiments. Journal of Helminthology 94, E122.CrossRefGoogle ScholarPubMed
Lucca, N, Stedille, F, Schwertz, C, Henker, L, Gabriel, M, Mendes, R, Pappen, F and Rosa, L (2015) Principais parasitoses gastrointestinais em bovinos provenientes de propriedades leiteiras de municípios do Alto Uruguai, Santa Catarina. Extensão Tecnológica: Revista de Extensão do Instituto Federal Catarinense 3, 63–68.CrossRefGoogle Scholar
Martin, OC (1972) The incidence of Eurytrema pancreaticum (Looss, 1907) in dairy cattle at the DTRI Farm. Philippine Agriculturist 56, 25–34.Google Scholar
Moriena, RA, Lombardero, OJ and Racioppi, O (1996) Eurytrema coelomaticum (Gerard & Billet, 1892) (Trematoda, Dicrocoeliidae) nuevo parásito del bovino para la Argentina. Revista de Medicina Veterinaria (Buenos Aires) 11, 247–249.Google Scholar
Sakamoto, T, Kono, I, Yasuda, N, Yamamoto, Y and Nakagawa, H (1980) Studies on Eurytrema coelomaticum. II. The anthelmintic efficiency of nitroxynil and praziquantel against Eurytrema coelomaticum in cattle. Memoirs of the Faculty of Agriculture, Kagoshima University 16, 93–101.Google Scholar
Viana, SS (1985) Técnica coproscópica de sedimentação para concentração de ovos de Eurytrema Looss, 1907. São Paulo, Tese (Doutorado) – Instituto de Ciências Biológicas, Universidade de São Paulo.Google Scholar
Viñabal, A, Cafrune Wierna, MM, Aguirre, DH, Bassanetti, AF, Bertoni, EA and Suarez, VH (2015) Propuesta y evaluación de una técnica de sedimentación y tinción con azul de metileno (y de una variante) para el diagnóstico de Fasciola hepatica. Veterinaria Argentina 32, 327.Google Scholar
Yeh, HY, Cheng, CFJ, Huang, C, Zhan, X, Wong, WK and Mitchell, PD (2019) Discovery of Eurytrema eggs in sediment from a colonial period latrine in Taiwan. The Korean Journal of Parasitology 57, 595.CrossRefGoogle ScholarPubMed