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Fractionation of Fasciola hepatica tegument antigens and their application to the serodiagnosis of experimental fascioliasis by the enzyme-linked immunosorbent assay

Published online by Cambridge University Press:  05 June 2009

George V. Hillyer  and
Adelfa E. Serrano
George V. Hillyer
Affiliation:
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Rio Piedras P. R. 00931, USA
Adelfa E. Serrano
Affiliation:
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Rio Piedras P. R. 00931, USA
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Abstract

A Fasciola hepatica tegument antigen preparation was obtained from intact adult worms by solubilization with a non-ionic detergent, Nonident P-40. This antigen preparation contains antigens useful for the serodiagnosis of infection with this parasite. However, the antigen preparation is inadequate for use in the enzyme-linked immunosorbent assay (ELISA). The present work demonstrates that fractionation by demulsification of this antigen preparation with ammonium sulphate results in a soluble aqueous phase which contains F. hepatica serodiagnostic antigens which can then be applied to the ELISA. This F. hepatica tegument antigen preparation when used m the ELISA can detect rabbit fascioliasis two weeks after infection, with antibody levels peaking by 10 to 12 weeks of infection.

Type
Research Article
Information
Journal of Helminthology , Volume 60 , Issue 3 , September 1986 , pp. 173 - 178
Copyright
Copyright © Cambridge University Press 1986

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References

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