Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-04T00:28:58.996Z Has data issue: false hasContentIssue false
  • English
  • Français

Isoenzymatic pattern of glucose 6-phosphate dehydrogenase from Ascaris suum

Published online by Cambridge University Press:  05 June 2009

C. Cutillas ,
B. Rodriguez ,
P. German  and
D. Guevara
C. Cutillas
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
B. Rodriguez
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
P. German
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
D. Guevara
Affiliation:
Departamento de Microbiologa y Parasitologia, Facultad de Farmacia, Unnersidad de Sevilla, Prof, Garcia González sln, 41012 Sevilla, Spain
Get access Rights & Permissions [Opens in a new window]

Abstract

The isoenzymatic pattern of glucose 6-phosphate dehydrogenase (abbreviation G6PD) from Ascaris suum has been studied by vertical polyacrylamide gel (PAGE) and horizontal starch gel electrophoresis. After polyacrylamide gel electrophoresis, two stained zones could be identified. One corresponded to tetrazolium oxidase activity; since this zone was stained even in the absence of glucose 6-phosphate (G6P), non-specific staining could be detected. In the other zone of activity, seven regularly-spaced bands were identified by staining in the presence of G6P and NADP as substrates. By using starch gel electrophoresis, different electrophoretic patterns for G6PD have been observed in the muscular sac, intestine and reproductive system from A. suum. The existence of three different alleles of G6PD in the same individual suggests the existence of at least two genes for this enzyme.

Keywords

Ascaris suumisoenzymatic patternPAGEstarch gel electrophoresisglucose 6-phosphate dehydrogenaseNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 226 - 232
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bullini, L. & Coluzzi, M. (1982) Evolutionary and taxonomic inferences of electrophoretic studies in mosquitoes. In: Developments in the Genetics of Disease Vectors. Stipes Publishing Company, Champaing, Illinois, pp. 465–482.Google Scholar
Davis, B. J. (1964) Disc electrophoresis II. Methods and application to human serum proteins. Annals of the New York Academy of Sciences, 121, 404–427.CrossRefGoogle ScholarPubMed
Fletcher, M. & Loverde, P. T. (1981) Schistosoma mansoni: Electrophoretic characterization of strains selected for different levels of infectivity to snails. Experimental Parasitology, 52, 362–370.CrossRefGoogle ScholarPubMed
Freeman, W. H. (1982) Physical Biochemistry, Applications to Biochemistry and Molecular Biology. W. H. Freeman & Co., San Francisco.Google Scholar
Friedman, P. A., Platzer, E. M. & Eby, J. E. (1977) Species differentiation in Caeriorhabditis briggsae and Caenorhabditis elegans. Journal of Neniatology, 9, 197–203.Google ScholarPubMed
Harris, H. & Hopkinson, D. A. (1976) Handbook of Enzyme Electrophoresis in Human Genetics. North Holland Publishing Company, Amsterdam.Google Scholar
Leslie, J. F., Cain, G. D., Heffe, G. K. & Vrijenhort, R. C. (1982) Enzyme polymorphism in Ascaris suum (Nematoda). Journal of Parasitology, 84, 576–587.CrossRefGoogle Scholar
Mydinski, L. J. & Dick, T. A. (1985) The use of enzyme polymorphism to identify genetic differences in the genus Trichinella. Journal of Parasitology, 71, 671–677.CrossRefGoogle Scholar
Nascetti, G., Grapelli, C. & Bullini, L. (1980) Ricerche sul differenziamento genetico di Ascaris lumbricoidese Ascaris suum. Accademia Nazionale dei Lincei, 6, 457–465.Google Scholar
Ohnishi, K. & Hori, S. H. (1977) A comparative study of invertebrate glucose 6-phosphate dehydrogenase. Japanese Journal of Genetics, 52, 95–106.Google Scholar
Orstein, L. (1964) Disc electrophoresis I. Background and theory. Annals of the New York Academy of Sciences, 121, 321–349.CrossRefGoogle Scholar
Paggi, L., Nascetfi, G., Orecchia, P., Mattiuci, S. & Bulini, L. (1985) Biochemical taxonomy of ascaridoid nematodes. Parassitologia, 27, 321–349.Google ScholarPubMed
Pasteur, N., Pasteur, G., Bonhomme, F., Catalan, J. & Britton-Davidian, J. (1988) Practical Isoenzyme Genetics. Ellis Horwood series in Gene technology. Halsted Press: a division of John Wiley & Sons. New York.Google Scholar
Podzol, R. E. & Noel, G. R. (1984) Comparative electrophoretic analyses of soluble proteins from Heterodera glycines races 1–4 and three other Heterodera species. Journal of Nematology. 16, 332–340.Google Scholar
Shaw, C. R. & Prasad, P. (1970) Starch gel electrophoresis. A compilation of recipes. Biochemical Genetics, 4, 297–320.CrossRefGoogle ScholarPubMed
Yoshida, A. (1975) Evolution of glucose 6-phosphate dehydrogenase and hexose 6-phosphate dehydrogenase. In: Isoenzyme Genetics and Evolution. Academic Press, San Francisco.Google Scholar