Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-25T09:49:48.211Z Has data issue: false hasContentIssue false
  • English
  • Français

Larval somatic antigens effective in inducing an IgG response and protection against Strongyloides papillosus infection

Published online by Cambridge University Press:  05 June 2009

C. Ooba ,
Y. Nakamura  and
N. Taira
C. Ooba
Affiliation:
Hokkaido Prefectural Ishikari Livestock Hygiene Service Center, Sapporo, Hokkaido 062, Japan
Y. Nakamura*
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
N. Taira
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
*
* Author for correspondence.
Get access Rights & Permissions [Opens in a new window]

Abstract

A serum IgG response to Strongyloides papillosus infection was analysed in rabbits. IgG production was induced against 30–200 kDa larval somatic antigens and 21–160 kDa adult excretory/secretory (ES) antigens at 4 weeks postinfection. No immunoreactivity was detected between sera of infected animals and any proteins of larval ES and adult somatic antigens. Protection against larval challenge infection was investigated in rabbits immunized by primary infection and by intradermal inoculations of larval somatic and adult ES antigens. Primarily infected animals had 90% fewer or less worms from day 2 onwards after challenge, and lower faecal egg outputs after challenge, when compared with those in susceptible animals. Immunization with larval somatic antigens induced effective protection, showing 52.6–81.5% reductions in worm recovery from day 5 onwards after challenge, and lower faecal egg outputs after challenge. Systemic immunization with adult ES antigens failed to protect the animals against challenge. The possibility that resistance to S. papillosus reinfection is performed on the killing of migrating larvae in the early stages of infection is discussed. Systemic immune responses to larval somatic antigens might play an important role in the resistance.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 3 , September 1996 , pp. 231 - 235
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Dawkins, H.J.S. & Grove, D.I. (1981) Transfer by serum and cells of resistance to infection with Strongyloides ratti in mice. Immunology 43, 317–322.Google Scholar
Grove, D.I., Northern, C. & Dawkins, H.J.S. (1985) Interactions of Strongyloides ratti free-living and skin-penetrated infective larvae and parasitic adults with serum and cells in vitro. Australian Journal of Experimental Biology and Medical Science 63, 521–529.CrossRefGoogle ScholarPubMed
Jaron, W. (1964) Immunity reaction of the rabbit against sheep and rabbit strains of Strongyloides papillosus in repeated infections. Acta Parasitologica Polonica 12, 133–149.Google Scholar
Lowry, O.K., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Mimori, T., Tanaka, M. & Tada, I. (1987) Strongyloides ratti: Formation of protection in rats by excretory/secretory products of adult worms. Experimental Parasitology 64, 342–346.CrossRefGoogle ScholarPubMed
Murrell, K.D. (1980) Strongyloides ratti: Acquired resistance in the rat to the preintestinal migrating larvae. Experimental Parasitology 50, 417–425.CrossRefGoogle Scholar
Murrell, K.D. (1981) Protective role of immunoglobulin G in immunity to Strongyloides ratti. Journal of Parasitology 67, 167–173.CrossRefGoogle ScholarPubMed
Nakamura, Y., Kawazu, S. & Minami, T. (1991) Analysis of protein compositions and surface protein epitopes of Anaplasma centrale and Anaplasma marginale. Journal of Veterinary Medical Science 53, 73–79.CrossRefGoogle ScholarPubMed
Nakamura, Y., Tsuji, N. & Taira, N. (1994a) Wasting condition under normal cardiac rhythms in rabbits following Strongyloides papillosus infection. Journal of Veterinary Medical Science 56, 1005–1007.CrossRefGoogle ScholarPubMed
Nakamura, Y., Tsuji, N., Taira, N. & Hirose, H. (1994b) Parasitic females of Strongyloides papillosus as a pathogenetic stage for sudden cardiac death in infected lambs. Journal of Veterinary Medical Science 56, 723–727.CrossRefGoogle ScholarPubMed
Northern, C. & Grove, D.I. (1988) Western blot analysis of reactivity to larval and adult Strongyloides ratti antigens in mice. Parasite Immunology 10, 681–691.CrossRefGoogle ScholarPubMed
Nwaorgu, O.C. & Connan, R.M. (1980) The migration of Strongyloides papillosus in rabbits following infection by the oral and subcutaneous routes. Journal of Helminthology 54, 223–232.CrossRefGoogle ScholarPubMed
Ratyñska-Prill, D. (1980) Immunity to strongyloidosis in experimentally infected goats. II. Immunological studies. Acta Parasitologica Polonica 27, 93–100.Google Scholar
Tsuji, N. & Fujisaki, K. (1994) Development in vitro of free-living infective larvae to the parasitic stage of Strongyloides venezuelensis by temperature shift. Parasitology 109, 643–648.CrossRefGoogle Scholar
Uchikawa, R., Ichiki, H. & Komaki, E. (1991) Antibody responses and protective immunity in rats receiving repeated inoculations of Strongyloides ratti. Journal of Parasitology 77, 737–741.CrossRefGoogle ScholarPubMed