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Larvicidal activity of Bacillus circulans against the gastrointestinal nematode Haemonchus contortus in sheep

Published online by Cambridge University Press:  29 December 2014

M.C. Sinott ,
L.L. Dias de Castro ,
F.L.L Leite ,
T. Gallina ,
M.T. De-Souza ,
D.F.L. Santos  and
F.P.L. Leite
M.C. Sinott
Affiliation:
Programa de Pós-Graduação em Parasitologia, Universidade Federal de Pelotas, Brazil
L.L. Dias de Castro
Affiliation:
Faculdade de Veterinária, Universidade Federal de Pelotas, Brazil
F.L.L Leite
Affiliation:
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA
T. Gallina
Affiliation:
Faculdade de Veterinária, Universidade Federal de Pelotas, Brazil
M.T. De-Souza
Affiliation:
Departamento de Biologia Celular, Universidade de Brasília, Brazil
D.F.L. Santos
Affiliation:
Departamento de Biologia Celular, Universidade de Brasília, Brazil
F.P.L. Leite*
Affiliation:
Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Brazil
*
*Fax: +55 (53) 3921 1268 E-mail: fabio_leite@ufpel.edu.br
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Abstract

Efficient control of gastrointestinal parasites is necessary in sheep breeding. However, the available chemically based anthelmintics are becoming less effective due to the development of parasite resistance. An alternative to this problem is biological control. In the present study, we tested the larvicidal effect of Bacillus circulans by administering a spore suspension (2 × 109 colony forming units/ml) orally to lambs naturally infected with Haemonchus contortus. The number of faecal larvae was quantified daily and a significant reduction (~87%, P< 0.05) of larval development was observed after administration of B. circulans. Using a transformed B. circulans with green fluorescent protein, we were able to detect B. circulans in the faeces at 4 h post-administration and 72 h after cessation of its administration. These results suggest the use of B. circulans as a promising biological alternative for parasite control.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 68 - 73
Copyright
Copyright © Cambridge University Press 2014 

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