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Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus

Published online by Cambridge University Press:  04 November 2016

M. Sindičić ,
F. Martinković ,
T. Strišković ,
M. Špehar ,
I. Štimac ,
M. Bujanić  and
D. Konjević
M. Sindičić
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
F. Martinković*
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
T. Strišković
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
M. Špehar
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
I. Štimac
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
M. Bujanić
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
D. Konjević
Affiliation:
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
*
*E-mail: franjo.martinkovic@vef.hr
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Abstract

Paramphistomosis, caused by paramphistomid flukes, is a gastrointestinal parasitic disease of domestic and wild ruminants. Originally thought to be limited to the tropics and subtropics, the disease has recently been reported in temperate regions. Here we describe the concurrent infection of a red deer doe (Cervus elaphus) with Paramphistomum leydeni and Paramphistomum cervi. This is the first report of P. leydeni in Croatia. Flukes were identified on the basis of morphological keys (tegumental papillae) and sequencing of the internal transcribed spacer region 2 in ribosomal DNA. Our results confirm that the absence of tegumental papillae allows P. cervi to be differentiated morphologically from other paramphistomid species in Europe based on incident light stereomicroscopy. Nevertheless the limitations of morphological identification and taxonomic issues suggest that previous findings on paramphistomid infection should be interpreted carefully. The possible worldwide distribution of these pathogens means that paramphistomosis may be more common and its economic impact greater than previously thought.

Type
Short Communications
Information
Journal of Helminthology , Volume 91 , Issue 5 , September 2017 , pp. 637 - 641
Copyright
Copyright © Cambridge University Press 2016 

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References

Anderson, G.R. & Barker, S.C. (1998) Inference of phylogeny and taxonomy within the Didymozoidae (Digenea) from the second internal transcribed spacer (ITS2) of ribosomal DNA. Systematic Parasitology 41, 87–94.Google Scholar
Bazsalovicsová, E., Králová-Hromadová, I., Špakulová, M., Reblánová, M. & Oberhauserová, K. (2010) Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia 47, 76–82.Google Scholar
Eduardo, S.L. (1982a) The taxonomy of the family Paramphistomidae Fischoeder, 1901 with special reference to the morphology of species occurring in ruminants. I. General considerations. Systematic Parasitology 4, 7–57.Google Scholar
Eduardo, S.L. (1982b) The taxonomy of the family Paramphistomidae Fischoeder, 1901 with special reference to the morphology of species occurring in ruminants. II. Revision of the genus Paramphistomum Fischoeder, 1901. Systematic Parasitology 4, 189–238.Google Scholar
Eduardo, S.L. (1984) The taxonomy of the family Paramphistomidae Fischoeder, 1901 with special reference to the morphology of species occurring in ruminants. IV. Revision of the genus Gigantocotyle Näsmark, 1937 and elevation of the subgenus Explanatum Fukui, 1929 to full generic status. Systematic Parasitology 6, 3–32.Google Scholar
Eduardo, S.L. (1985) The taxonomy of the family Paramphistomidae Fischoeder, 1901 with special reference to the morphology of species occurring in ruminants. V. Revision of the genus Cotylophoron Stiles & Goldberger, 1910. Systematic Parasitology 7, 3–26.CrossRefGoogle Scholar
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/97/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Lotfy, W.M., Brant, S.V., Ashmawy, K.I., Devkota, R., Mkoji, G.M. & Loker, E.S. (2010) A molecular approach for identification of paramphistomes from Africa and Asia. Veterinary Parasitology 174, 234–240.Google Scholar
Luton, K., Walker, D. & Blair, D. (1992) Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminths: Trematoda Digenea). Molecular and Biochemical Parasitology 56, 323–328.Google Scholar
Ma, J., He, J.J., Liu, G.H., Zhou, D.H., Liu, J.Z., Liu, Y. & Zhu, X.Q. (2015) Mitochondrial and nuclear ribosomal DNA dataset supports that Paramphistomum leydeni (Trematoda: Digenea) is a distinct rumen fluke species. Parasites & Vectors 8, 201. doi.org/10.1186/s13071-015-0823-4.CrossRefGoogle ScholarPubMed
Nikander, S. & Saari, S. (2007) Notable seasonal variation observed in the morphology of the reindeer rumen fluke (Paramphistomum leydeni) in Finland. Rangifer 27, 47–57.Google Scholar
O'Toole, A., Browne, J.A., Hogan, S., Bassière, T., DeWaal, T., Mulcahy, G. & Zintl, A. (2014) Identity of rumen fluke in deer. Parasitology Research 113, 4097–4103.CrossRefGoogle ScholarPubMed
Pavlović, I., Savić, B., Ivanović, S. & Ćirović, D. (2012) First occurrence of Paramphistomum microbothrium (Fischoeder 1901) in roe deer (Capreolus capreolus) in Serbia. Journal of Wildlife Disease 48, 520–522.Google Scholar
Sanabria, R.E.F. & Romero, J.R. (2008) Review and update of paramphistomosis. Helminthologia 45, 64–68.Google Scholar
Sanabria, R., Moré, G. & Romero, J. (2011) Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae). Veterinary Parasitology 177, 182–185.CrossRefGoogle Scholar
Taylor, M.A., Coop, R.L. & Wall, R.L. (2007) Veterinary parasitology. 3rd edn. London, Wiley-Blackwell.Google Scholar
Thompson, J.D., Higgins, D. & Gibson, T.J. (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.Google Scholar
Zheng, X., Chang, Q.C., Zhang, Y., Tian, S.Q., Lou, Y., Duan, H., Guo, D.H., Wang, C.R. & Zhu, X.Q. (2014) Characterization of the complete nuclear ribosomal DNA sequences of Paramphistomum cervi . The Scientific World Journal. dx.doi.org/10.1155/2014/751907.Google Scholar