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Molecular phylogenetic relationships of Metastrongylus nematodes with emphasis on specimens from domestic pigs in Vietnam

Published online by Cambridge University Press:  06 September 2021

N.V. Tuyen ,
N.T.K. Lan  and
P.N. Doanh Open the ORCID record for P.N. Doanh [Opens in a new window]
N.V. Tuyen
Affiliation:
Dien Bien Technical Economic College, Dien Bien Phu city, 380000, Vietnam
N.T.K. Lan
Affiliation:
Thai Nguyen University of Agriculture and Forestry, Thai Nguyen city, 250000, Vietnam
P.N. Doanh*
Affiliation:
Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology (VAST), Hanoi, 122100, Vietnam Graduate University of Sciences and Technology, VAST, Hanoi, 122100, Vietnam
*
Author for correspondence: P.N. Doanh, E-mail: pndoanh@yahoo.com
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Abstract

Lungworms of the genus Metastrongylus are parasitic nematodes in the respiratory tract of swine. Although they infect both wild boars and domestic pigs, studies on Metastrongylus infections in wild boars in Europe, the Americas and Africa are numerous, while those in domestic pigs are few. There are several studies analysing the molecular phylogenetic relationships of few individual Metastrongylus species with other nematode taxa, but there are no studies on the phylogenetic relationships of species within the genus Metastrongylus. In Southeast Asia, reports on swine lungworms are extremely scarce and do not include any nucleotide sequence data. Therefore, the aim of the present study is to survey Metastrongylus infection in domestic pigs raised in Dien Bien Province, Northern Vietnam, and to analyse the molecular phylogenetic relationships of Metastrongylus species. Based on morphological and molecular data, we identified two species: Metastrongylus apri and Metastrongylus pudendotectus. The prevalence of the former species was found to be significantly higher than the latter one (24.1% vs. 2.3%). We observed pigs exhibiting a coinfection with the two lungworm species or a single infection with only M. apri. However, we did not observe any pigs being infected with just M. pudendotectus. Vietnamese Metastrongylus specimens showed slight morphological and molecular differences compared to those from other countries. The molecular analyses revealed a close genetic relationship between M. apri and Metastrongylus salmi, while both these species were far distant from M. pudendotectus. The present study highlights the needs for further studies to clarify the morphological features and ecological and phylogenetic relationships of Metastrongylus species at the global scale.

Keywords

Molecular analysismorphologyinfectionMetastrongylus apriMetastrongylus pudendotectus
Type
Short Communication
Information
Journal of Helminthology , Volume 95 , 2021 , e52
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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