Hostname: page-component-84b7d79bbc-rnpqb Total loading time: 0 Render date: 2024-07-29T14:20:44.317Z Has data issue: false hasContentIssue false
  • English
  • Français

Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected rats

Published online by Cambridge University Press:  22 May 2015

A.L.R. Gonçalves ,
K.C.L. de Araújo ,
E.F.G. Carvalho ,
M.T. Ueta  and
J.M. Costa-Cruz
A.L.R. Gonçalves
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, 38400-902, Minas Gerais, Brazil
K.C.L. de Araújo
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, 38400-902, Minas Gerais, Brazil
E.F.G. Carvalho
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, 38400-902, Minas Gerais, Brazil
M.T. Ueta
Affiliation:
Laboratório de Parasitologia, Universidade Estadual de Campinas, Caixa Postal 6109, CEP 13083-970, Campinas, São Paulo, Brazil
J.M. Costa-Cruz*
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, 38400-902, Minas Gerais, Brazil
*
*E-mail: costacruz@ufu.br
Get access Rights & Permissions [Opens in a new window]

Abstract

In the present study, antigens from parthenogenetic females and eggs of Strongyloides venezuelensis, or anti-parthenogenetic-female and anti-egg antigens were used to detect specific IgG and immune complex responses, respectively. Serum samples from experimentally infected immunocompetent and immunosuppressed rats were analysed on days 5, 8, 13 and 21 post-infection (dpi). An enzyme-linked immunosorbent assay (ELISA) was performed using alkaline parasite extract for specific IgG detection, and anti-parthenogenetic-female or anti-egg antigens for immune complex detection. The data were analysed using analysis of variance (ANOVA), followed by a Bonferroni test. When parthenogenetic female or egg extracts were used as antigens, specific IgGs were not detected in either immunocompetent or immunosuppressed rats. When anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used, immune complexes were detected for the duration of the infection in immunosuppressed animals and were only detected between 5 and 13 dpi in immunocompetent animals. The duration of infection was not significantly different between the immunocompetent and immunosuppressed groups when anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used. Parthenogenetic female extracts yielded significant differences between antibody and immune complex responses in immunocompetent rats from 5 to 13 dpi, but only on day 5 dpi in immunosuppressed rats. Exposure to S. venezuelensis egg extract yielded significant differences in both antibody and immune complex detection between immunocompetent and immunosuppressed rats for the duration of the infection. In conclusion, ELISA using alternative antigens may be a successful strategy for identifying immune complexes in serum samples and diagnosing active strongyloidiasis, particularly under conditions of immunosuppression.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 3 , May 2016 , pp. 342 - 346
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Gonçalves, A.L.R., Ribeiro, T.S., Silva, C.V., Ueta, M.T. & Costa-Cruz, J.M. (2012a) A novel approach based on antigen, antibody and immune complex detection in bronchoalveolar lavage fluid samples from rats experimentally infected with Strongyloides venezuelensis . Acta Tropica 124, 166–169.Google Scholar
Gonçalves, A.L.R., Rocha, C.A., Gonzaga, H.T., Gonçalves-Pires, M.R.F., Ueta, M.T. & Costa-Cruz, J.M. (2012b) Specific IgG and IgA to larvae, parthenogenetic females and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis. Diagnostic Microbiology and Infectious Disease 72, 79–84.Google Scholar
Gonçalves, A.L.R., Silva, C.V., Ueta, M.T. & Costa-Cruz, J.M. (2012c) Antigen, antibody and immune complex detection in serum samples from rats experimentally infected with Strongyloides venezuelensis . Experimental Parasitology 130, 205–208.Google Scholar
Gonzaga, H.T., Ribeiro, V. da S., Cunha-Júnior, J.P., Ueta, M.T. & Costa-Cruz, J.M. (2011) Usefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasis. Diagnostic Microbiology and Infectious Disease 70, 78–84.Google Scholar
Gordon, H.M. & Whitlock, H.V.A. (1939) New technique for counting nematode eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research 12, 50–52.Google Scholar
Grove, D.I. (1996) Human strongyloidiasis. Advances in Parasitology 38, 251–309.CrossRefGoogle ScholarPubMed
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Machado, E.R., Ueta, M.T., Gonçalves-Pires, M.R.F., Oliveira, J.B.A., Faccioli, L.H. & Costa-Cruz, J.M. (2003) Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay. Memórias do InstitutoOswaldo Cruz 98, 849–851.CrossRefGoogle ScholarPubMed
Paula, F.M., Sitta, R.B., Malta, F.M., Gottardi, M., Corral, M.A., Gryschek, R.C.B. & Chieffi, P.P. (2013) Parasitological and molecular diagnosis in experimental Strongyloides venezuelensis infection. Revista do Instituto de Medicina Tropical de São Paulo 55, 141–143.Google Scholar
Rodrigues, R.M., Cardoso, C.R., Gonçalves, A.L.R., Silva, N.M., Massa, V., Alves, R., Ueta, M.T., Silva, J.S. & Costa-Cruz, J.M. (2013) Increased susceptibility to Strongyloides venezuelensis infection is related to the parasite load and absence of major histocompatibility complex (MHC) class II molecules. Experimental Parasitology 135, 580–586.Google Scholar
Romand, S., Thulliez, P. & Dubey, J.P. (1998) Direct agglutination test for serologic diagnosis of Neospora caninum infection. Parasitology Research 84, 50–53.Google Scholar
Rugai, E., Mattos, T. & Brisola, A.P. (1954) Nova técnica para isolar larvas de nematóides das fezes: Modificação do método de Baermann. Revista do Instituto Adolfo Lutz 14, 5–8.Google ScholarPubMed
Sato, Y. & Toma, H. (1990) Strongyloides venezuelensis infections in mice. International Journal of Parasitology 20, 57–62.Google Scholar
Wilson, M.B. & Nakane, P.K. (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. p. 215 in Knapp, W., Holuban, K. & Wick, G. (Eds) Immunofluorescence and related techniques. Amsterdam, North-Holland Biomedical.Google Scholar
Yasuda, K., Matsumoto, M. & Nakanishi, K. (2014) Importance of both innate immunity and acquired immunity for rapid expulsion of S. venezuelensis . Frontiers in Immunology 5, 1–5.CrossRefGoogle ScholarPubMed