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Estimation of the numbers and infectivity of Nippostrongylus larvae

Published online by Cambridge University Press:  05 June 2009

Anne Keymer ,
Jean Martin  and
S. M. Wainwright
Anne Keymer
Affiliation:
University of Cambridge, Department of Parasitology, Molteno Institute, Downing Street, Cambridge CB2 3EE
Jean Martin
Affiliation:
University of Cambridge, Department of Parasitology, Molteno Institute, Downing Street, Cambridge CB2 3EE
S. M. Wainwright
Affiliation:
University of Cambridge, Department of Parasitology, Molteno Institute, Downing Street, Cambridge CB2 3EE
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Abstract

Experimental investigations of the subcutaneous infection of third-stage larvae of Nippostrongylus brasiliensis reveal discrepancies between the actual and intended inoculum, and also variability between replicate inocula. Emphasis is thus placed on the importance of obtaining accurate estimates of the level of confidence associated with inoculum size. Under specified laboratory conditions, the retention of infectivity of N. brasiliensis larvae was found to be age-dependent, with a maximum of between 120 and 156 days. The motility of the larvae was not found to be indicative of their ability to complete migration and to become established in the small intestine of the host following subcutaneous inoculation.

Type
Research Papers
Information
Journal of Helminthology , Volume 57 , Issue 3 , September 1983 , pp. 225 - 230
Copyright
Copyright © Cambridge University Press 1983

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References

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