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Fasciola antigens as vaccines against fascioliasis and schistosomiasis

Published online by Cambridge University Press:  12 April 2024

G.V. Hillyer
G.V. Hillyer*
Affiliation:
Department of Pathology and Laboratory Medicine, University of Puerto Rico School of Medicine, PO Box 365067, San Juan, PR 00936-5067, Puerto Rico
*
*Author for correspondence Fax: (787) 751 9210 E-mail: ghillyer@rcm.upr.edu
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Abstract

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Fascioliasis is an important trematode infection of herbivores worldwide with increasing evidence of prevalence as a disease of humans. Vaccination studies with purified native and recombinant Fasciola antigens suggest that this approach to diminished morbidity and mortality and reduced transmission is a realistic goal. Among the major potential vaccine candidates are fatty acid binding protein (FABP), cysteine (cathepsin) proteases, haemoglobulin, leucine aminopeptidase, and a saposin-like protein. In the case of Fasciola hepatica FABP, cross-reaction and cross-protection against Schistosoma mansoni is an important feature. In addition to protective effects with significant worm burden reductions, some vaccine candidates also have anti-fecundity (smaller flukes), anti-pathology (less liver lesions), and anti-embryonation effects. Optimism is tempered by the fact that fascioliasis in humans is an orphan disease and in need of governmental and foundation support.

Type
Review Article
Information
Journal of Helminthology , Volume 79 , Issue 3 , September 2005 , pp. 241 - 247
Copyright
Copyright © Cambridge University Press 2005

References

Almeida, M.S., Torloni, H., Lee-Ho, P., Vilar, M.M., Taumaturgo, N., Simpson, A.J. & Tendler, M. (2003) Vaccination against Fasciola hepatica infection using a Schistosoma mansoni defined recombinant antigen, Sm14. Parasite Immunology 25, 135–137.CrossRefGoogle ScholarPubMed
Bjorland, J., Bryan, R.T., Strauss, W., Hillyer, G.V. & McAuley, J.B. (1995) An outbreak of acute fascioliasis among Aymara Indians in the Bolivian Altiplano. Clinical Infectious Diseases 21, 1228–1233.CrossRefGoogle ScholarPubMed
Casanueva, P., Hillyer, G.V., Ramajo, V., Oleaga, A., Espinoza, E.Y. & Muro, A. (2001) Immunoprophylaxis against Fasciola hepatica in rabbits using a recombinant Fh15 fatty acid-binding protein. Journal of Parasitology 87, 697–700.CrossRefGoogle ScholarPubMed
Curtale, F., Hammoud, E.S., Wakeel, A., Mas-Coma, S. & Savioli, L (2000) Human fascioliasis, an emerging public health problem in the Nile Delta, Egypt. Research and Reviews in Parasitology 60, 129–134.Google Scholar
Dalton, J.P., Brindley, P.J., Knox, D.P., Brady, C.P., Hotez, P.J., Donnelly, S., O'Neill, S.M., Mulcahy, G. & Loukas, A. (2003 a) Helminth vaccines: from mining genomic information for vaccine targets to systems used for protein expression. International Journal for Parasitology 33, 621–640.CrossRefGoogle ScholarPubMed
Dalton, J.P., Neill, S.O., Stack, C., Collins, P., Walshe, A., Sekiya, M., Doyle, S., Mulcahy, G., Hoyle, D., Khaznadji, E., Moire, N., Brennan, G., Mousley, A., Kreshenko, N., Maules, A.G. & Donnely, S.M. (2003 b) Fasciola hepatica cathepsin L-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines. International Journal for Parasitology 33, 1173–1181.CrossRefGoogle ScholarPubMed
De Bont, J., Cleerebout, E., Riveau, G., Schacht, A.M., Smets, K., Conder, G., Brake, D.A., Capron, A. & Vercruysse, J. (2003) Failure of a recombinant Schistosoma bovis -derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection. Veterinary Parasitology 113, 135–144.CrossRefGoogle ScholarPubMed
Espino, A.M. & Hillyer, G.V. (2001) Isolation and immunological characterization of fatty acid binding protein isoforms from Fasciola hepatica . Journal of Parasitology 87, 1028–1033.CrossRefGoogle ScholarPubMed
Espino, A.M. & Hillyer, G.V. (2003) Molecular cloning of a member of the Fasciola hepatica saposin-like protein family. Journal of Parasitology 89, 545–552.CrossRefGoogle ScholarPubMed
Espino, A.M. & Hillyer, G.V. (2004) A novel Fasciola hepatica saposinlike recombinant protein with immunoprophylactic potential. Journal of Parasitology 90, 876–879.CrossRefGoogle ScholarPubMed
Espino, A.M., Gil, R., Osuna, A. & Hillyer, G.V. (2005) Fasciola hepatica: humoral and cytokine responses to a member of the saposin-like protein family following delivery as a DNA vaccine in mice. Experimental Parasitology in press.CrossRefGoogle Scholar
Esteban, J.G., Bargues, M.D. & Mas-Coma, S. (1998) Geographical distribution, diagnosis and treatment of human fascioliasis: a review. Research and Reviews in Parasitology 58, 13–42.Google Scholar
Estuningsih, S.E., Smooker, P.M., Wiedosari, E., Widjajanti, S., Vaiano, S., Partoutomo, S. & Spithill, T.W. (1997) Evaluation of antigens of Fasciola gigantica as vaccines against tropical fasciolosis in cattle. International Journal for Parasitology 27, 1419–1428.CrossRefGoogle ScholarPubMed
Harmsen, M.M., Cornelissen, J.B., Buijs, H.E., Boersma, W.J., Jeurissen, S.H. & van Milligen, F.J. (2004) Identification of a novel Fasciola hepatica cathepsin L protease containing protective epitopes within the propeptide. International Journal for Parasitology 34, 675–682.CrossRefGoogle ScholarPubMed
Haroun, E.T.M. & Hillyer, G.V. (1986) Resistance to fascioliasis: a review. Veterinary Parasitology 20, 63–93.CrossRefGoogle ScholarPubMed
Hien, T.V., Dung, T.T.K., Chi, N.H., Dahn, P.H. & Pham, P.T. (2001) Fascioliasis in Vietnam. South East Asian Journal of Tropical Medicine and Public Health 31 (Supplement 2), 48–50.Google Scholar
Hillyer, G.V. (1976) Can we vaccinate against schistosomes? Federation Proceedings, American Societies for Experimental Biology 35, 2568–2571.Google ScholarPubMed
Hillyer, G.V. (1979) Schistosoma mansoni: reduced worm burdens in mice immunized with isolated Fasciola hepatica antigens. Experimental Parasitology 48, 287–296.CrossRefGoogle ScholarPubMed
Hillyer, G.V. (1984) Immunity to schistosomes using heterologous trematode antigens. Veterinary Parasitology 14, 263–283.CrossRefGoogle ScholarPubMed
Hillyer, G.V. (1985) Induction of immunity in mice to Fasciola hepatica with a Fasciola/Schistosoma cross-reactive defined immunity antigen. American Journal of Tropical Medicine and Hygiene 34, 1127–1131.CrossRefGoogle ScholarPubMed
Hillyer, G.V. (1987) Heterologous resistance in schistosomiasis. Memorias do Instituto Oswaldo Cruz 82 (Supplement IV), 171–174.CrossRefGoogle ScholarPubMed
Hillyer, G.V. (1995) Comparison of purified 12 kDa and recombinant 15 kDa Fasciola hepatica antigens related to a Schistosoma mansoni fatty acid binding protein. Memorias do Instituto Oswaldo Cruz 90, 249–253.CrossRefGoogle ScholarPubMed
Hillyer, G.V., Haroun, E.T.M., Hernandez, A. & Soler de Galanes, M. (1987) Acquired resistance to Fasciola hepatica in cattle using a purified adult worm antigen. American Journal of Tropical Medicine and Hygiene 37, 363–369.CrossRefGoogle ScholarPubMed
Hillyer, G.V., Garcia Rosa, M.I., Alicea, H. & Hernandez, A. (1988 a) Successful vaccination against murine Schistosoma mansoni infection with a purified 12 kD Fasciola hepatica cross-reactive antigen. American Journal of Tropical Medicine and Hygiene 38, 103–110.CrossRefGoogle ScholarPubMed
Hillyer, G.V., Soler de Galanes, M., Garcia Rosa, M.I. & Montealegre, F. (1988 b) Acquired immunity in schistosomiasis with purified Fasciola hepatica cross-reactive antigens. Veterinary Parasitology 29, 265–280.CrossRefGoogle ScholarPubMed
Hillyer, G.V., Soler de Galanes, M., Rodríguez-Perez, J., Bjorland, J., Silva de Lagrava, M., Ramirez Guzman, S. & Bryan, R.T. (1992) Use of FAST-ELISA and EITB to determine the prevalence of human fascioliasis in the Bolivian Altiplano. American Journal of Tropical Medicine and Hygiene 46, 603–609.CrossRefGoogle ScholarPubMed
Hillyer, G.V. & Apt, W. (1997) Food-borne trematode infections in the Americas. Parasitology Today 13, 87–88.CrossRefGoogle Scholar
Kofta, K.W., Mieszczanek, J., Plucienniczak, G. & Wedrychowicz, H. (2000) Successful DNA immunization of rats against fascioliasis. Vaccine 18, 2985–2990.CrossRefGoogle Scholar
Lopez-Aban, J., Ramajo, V., Perez Arellano, J.L., Oleaga, A., Hillyer, G.V. & Muro, A. (1999) A fatty acid binding protein from Fasciola hepatica induced protection in C57/BL mice from challenge infection with Schistosoma bovis. Veterinary Parasitology 83, 107–121.CrossRefGoogle Scholar
Lopez-Aban, J., Oleaga, A., Ramajo, V., Casanueva, P., Perez Arellano, J.L., Hillyer, G.V. & Muro, A. (2000) Vaccination of mice against Schistosoma bovis with a recombinant fatty acid binding protein from Fasciola hepatica . Veterinary Parasitology 91, 33–42.CrossRefGoogle Scholar
Martinez-Fernandez, A.R., Nogal-Ruiz, J.J., Lopez-Aban, J., Ramajo, V., Oleaga, A., Manga-Gonzalez, Y., Hillyer, G.V. & Muro, A. (2004) Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD. Veterinary Parasitology 126, 287–298.CrossRefGoogle ScholarPubMed
Mas-Coma, S. & Bargues, M.D. (1997) Human liver flukes: a review. Research and Reviews in Parasitology 57, 145–218.Google Scholar
Mas-Coma, S., Angles, R., Esteban, J.G., Bargues, M.D., Buchon, P., Franjen, M. & Strauss, W. (1999) The Northern Bolivian Altiplano: a region highly endemic for human fascioliasis. Tropical Medicine and International Health 4, 454–467.CrossRefGoogle ScholarPubMed
Moghaddam, A.S., Massoud, J., Mahmoodi, M., Mahvi, A.H., Periago, M.V., Artigas, P., Fuentes, M.V., Bargues, M.D. & Mas-Coma, S. (2004) Human and animal fascioliasis in Mazandaran province, northern Iran. Parasitology Research 94, 61–69.CrossRefGoogle ScholarPubMed
Moser, D., Tendler, M., Griffiths, G. & Klinkert, M.-Q (1991) A 14-kDa Schistosoma mansoni polypeptide is homologous to a gene family of fatty acid binding proteins. Journal of Biological Chemistry 266, 8447–8454.CrossRefGoogle ScholarPubMed
Mulcahy, G. & Dalton, J.P. (2001) Cathepsin L proteinases as vaccines against infection with Fasciola hepatica (liver fluke) in ruminants. Research in Veterinary Science 70, 83–86.CrossRefGoogle ScholarPubMed
Mulcahy, G., O'Connor, F., McGonigle, S., Dowd, A., Clery, D.G., Andrews, S.J. & Dalton, J.P. (1998) Correlation of specific antibody titre and avidity with protection in cattle immunized against Fasciola hepatica . Vaccine 16, 93–99.CrossRefGoogle ScholarPubMed
Muro, A., Ramajo, V., Lopez, J., Simon, F. & Hillyer, G.V. (1997) Fasciola hepatica: vaccination of rabbits with native and recombinant antigens related to fatty acid binding proteins. Veterinary Parasitology 69, 219–229.CrossRefGoogle ScholarPubMed
Paykari, H., Dalimi, A. & Madani, R. (2002) Immunization of sheep against Fasciola gigantica with glutathione S-transferase. Veterinary Parasitology 105, 153–159.CrossRefGoogle ScholarPubMed
Piacenza, O., Acosta, D., Basmadjian, I., Dalton, J.P. & Carmona, C. (1999) Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep. Infection and Immunity 67, 1954–1961.CrossRefGoogle ScholarPubMed
Ramajo, V., Oleaga, A., Casanueva, P., Hillyer, G.V. & Muro, A. (2001) Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins. Veterinary Parasitology 97, 35–46.CrossRefGoogle ScholarPubMed
Reed, M.B., Strugnell, R.A., Pannacio, M. & Spithill, T.W. (2000) A novel member of the NK-lysin protein family is developmentally regulated and secreted by Fasciola hepatica . Moecular and Biochemical Parasitology 105, 297–303.CrossRefGoogle ScholarPubMed
Rodriguez-Perez, J., Rodriguez-Medina, J.R., Garcia-Blanco, M.A. & Hillyer, G.V. (1992) Fasciola hepatica: molecular cloning, nucleotide sequence, and expression of a gene encoding a polypeptide homologous to a Schistosoma mansoni fatty acid-binding protein. Experimental Parasitology 74, 400–407.CrossRefGoogle ScholarPubMed
Sirisriro, S., Grams, R., Vichasri-Grams, S., Ardseungneon, P., Pankao, V., Meepool, A., Chaithirayanon, K., Viyanant, V., Tan-Ariya, P., Upatham, E.S. & Sobhon, P. (2002) Production and characterization of a monoclonal antibody against recombinant fatty acid binding protein of Fasciola gigantica . Veterinary Parasitology 105, 119–129.CrossRefGoogle ScholarPubMed
Smooker, P.M., Steper, K.R., Drew, D.R., Strugnell, R.A. & Spithill, T.W. (1999) Humoral responses in mice following vaccination with DNA encoding glutathione S-transferase of Fasciola hepatica: effects of mode of vaccination and the cellular compartment of antigen expression. Parasite Immunology 321, 357–364.CrossRefGoogle Scholar
Smooker, P.M., Kennedy, N.J., Steeper, K.R., Christopoulos, H. & Spithill, T.W. (2001) Fasciola: kinetics and quality of humoral responses to fatty acid binding protein and cathepsin L following delivery as DNA vaccines in mice. Experimental Parasitology 97, 154–160.CrossRefGoogle ScholarPubMed
Spithill, T.W. & Dalton, J.P. (1998) Progress in the development of liver fluke vaccines. Parasitology Today 14, 224–228.CrossRefGoogle ScholarPubMed
Spithill, T.W., Smooker, P.M. & Copeman, D.B. (1999 a) Fasciola gigantica: epidemiology, control, immunology, and molecular biology. pp. 465–525 in Dalton, J.P. (Ed.) Fasciolosis . Wallingford, Oxon, CAB International.Google Scholar
Spithill, T.W., Smooker, P.M., Sexton, J.L., Bozas, E., Nirrusib, C.S., Creany, J. & Parsons, J.C. (1999 b) Development of vaccines against Fasciola hepatica. pp. 377–410 in Dalton, J.P. (Ed.) Fasciolosis . Wallingford, Oxon, CAB International.Google Scholar
Tendler, M., Brito, C.A., Vilar, M.M., Serra-Freire, N., Diogo, C.M., Almeida, M.S., Delbem, A.C., Da Silva, J.F., Savino, W., Garratt, R.C., Katz, N. & Simpson, A.S. (1996) A Schistosoma mansoni fatty acid-binding protein, Sm14, is the potential basis of a dual-purpose anti-helminth vaccine. Proceedings of the National Academy of Sciences, USA 93, 269–273.CrossRefGoogle ScholarPubMed
Van Milligen, F.J., Cornelissen, J. & Bokhout, B.A. (2000) Fasciola hepatica: an antigen fraction derived from newly excysted juveniles, containing an immunoreactive 32-kDa protein, induces strong protective immunity in rats. Experimental Parasitology 94, 163–171.CrossRefGoogle ScholarPubMed
Vilar, M.M., Barrientos, F., Almeida, M., Taumaturgo, N., Simpson, A., Garrat, R. & Tendler, M. (2003) An experimental bivalent peptide vaccine against schistosomiasis and fascioliasis. Vaccine 22, 137–144.CrossRefGoogle ScholarPubMed
Wedrychowicz, H., Lamparska, M., Kesik, M., Kotomski, G., Mieszczanek, J., Jedlina-Panasiuk, L. & Plucienniczak, A. (2003) The immune response of rats to vaccination with the cDNA or protein forms of the cysteine proteinase of Fasciola hepatica . Veterinary Immunology and Immunopathology 94, 83–93.CrossRefGoogle ScholarPubMed