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Mitochondrial DNA differentiation of Japanese diploid and triploid Paragonimus westermani

Published online by Cambridge University Press:  05 June 2009

T. Agatsuma
Affiliation:
Department of Parasitology, Kochi Medical School, Nankoku City, Kochi 783, Japan
L. Yang
Affiliation:
Department of Medical Chemistry, Kochi Medical School, Nankoku City, Kochi 783, Japan
D. Kim
Affiliation:
National Institute of Health, Eunpyung-Ku, Seoul, Korea
H. Yonekawa
Affiliation:
Department of Laboratory of Animal Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113, Japan

Abstract

Restriction endonuclease analysis of mitochondrial DNA was carried out in order to differentiate diploid and triploid types of Paragonimus westermani originating from Japan. It was found that out of 16 restriction enzymes. PstI, HaeIII and RsaI revealed consistently different cleavage patterns between the two types.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1994

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References

Agatsuma, T. & Habe, S. (1985) Electrophoretic studies on enzymes of diploid and triploid Paragonimus ohirai. Parasitology 91, 489497.CrossRefGoogle Scholar
Attardi, G. (1985) Animal mitochondrial DNA: An extreme example of genetic economy. International Review of Cytology 93, 93145.CrossRefGoogle ScholarPubMed
Hirai, H. & Agatsuma, T. (1991) Triploidy in Paragonimus westermani. Parasitology Today 7, 1921.CrossRefGoogle ScholarPubMed
Hirai, H., Habe, S., Agatsuma, T. & Kawashima, K. (1992) Reciprocal translocation between the long arm of chromosomes 4 and 6 of Paragonimus westermani (Trematoda; Platyhelminthes) from the Philippines. Journal of Parasitology 78, 544546.CrossRefGoogle Scholar
Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503517.CrossRefGoogle ScholarPubMed
Tamura, K. & Aotsuka, T. (1988) Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure. Biochemical Genetics 26, 815819.CrossRefGoogle ScholarPubMed
Wang, E., Zheng, R. & Cain, G.D. (1991) Comparative studies on the repetitive DNA sequences of diploid and triploid forms of Paragonimus westermani by restriction endonuclease and Southern blotting. Chinese Journal of Parasitology and Parasitic Diseases 9, 4649.Google ScholarPubMed