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The serodiagnosis of human hydatid disease: 2. Additional studies on selected sera using indirect haemagglutination (IHA), enzyme linked immunosorbent assay (ELISA) and defined antigen substrate spheres (DASS)

Published online by Cambridge University Press:  05 June 2009

R. M. Matossian ,
Moira L. McLaren ,
C. C. Draper ,
C. M. Patricia Bradstreet ,
Mabel W. Dighero ,
G. J. Kane ,
L. M. Mackinlay  and
M. D. Rickard
R. M. Matossian
Affiliation:
Ross Institute of Tropical Hygiene, London School of Hygiene and Tropical Medicine, London WC1E 7HT
Moira L. McLaren
Affiliation:
Ross Institute of Tropical Hygiene, London School of Hygiene and Tropical Medicine, London WC1E 7HT
C. C. Draper
Affiliation:
Ross Institute of Tropical Hygiene, London School of Hygiene and Tropical Medicine, London WC1E 7HT
C. M. Patricia Bradstreet
Affiliation:
Standards Laboratory, Central Public Health Laboratory, London NW9 5HT
Mabel W. Dighero
Affiliation:
Standards Laboratory, Central Public Health Laboratory, London NW9 5HT
G. J. Kane
Affiliation:
Wellcome Research Laboratories, Beckenham, Kent
L. M. Mackinlay
Affiliation:
Wellcome Research Laboratories, Beckenham, Kent
M. D. Rickard
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
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Abstract

Sixty-one serum samples selected on the basis of reactivity in the complement fixation (CF) and latex agglutination (LA) test, were further examined for sensitivity and specificity by indirect haemagglutination (IHA), enzyme linked immunosorbent assay (ELISA) and defined antigen substrate spheres (DASS). Twenty sera from healthy Europeans and 48 samples from patients with either schistosomiasis or trichinosis were also tested. Comparable levels of sensitivity were found between the CF and LA positive sera and IHA, ELISA and DASS. Of the CF positive LA negative group of sera, many were positive by DASS but only a few reacted in IHA and ELISA. Some cross reactivity was also observed in the schistosomiasis sera tested by IHA and ELISA.

Type
Research Papers
Information
Journal of Helminthology , Volume 53 , Issue 4 , December 1979 , pp. 287 - 291
Copyright
Copyright © Cambridge University Press 1979

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