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The use of albendazole and diammonium glycyrrhizinate in the treatment of eosinophilic meningitis in mice infected with Angiostrongylus cantonensis

Published online by Cambridge University Press:  13 December 2011

Y. Li ,
J.-P. Tang ,
D.-R. Chen ,
C.-Y. Fu ,
P. Wang ,
Z. Li ,
W. Wei ,
H. Li  and
W.-Q. Dong
Y. Li
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China Wuxi Hospital for Infectious Diseases, Wuxi214005, Jiangsu, People's Republic of China Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou730000, Gansu, People's Republic of China
J.-P. Tang
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
D.-R. Chen
Affiliation:
Department of Pathology, Zhangye Municipality People's Hospital, Zhangye734000, Gansu, People's Republic of China
C.-Y. Fu
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
P. Wang
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
Z. Li
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
W. Wei
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
H. Li
Affiliation:
Department of Etiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
W.-Q. Dong*
Affiliation:
Department of Biopharmaceuticals, School of Biotechnology, Southern Medical University, Guangzhou510515, Guangdong, People's Republic of China
*
* Fax: +86 02061648556, E-mail: dongwq63@263.net
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Abstract

Angiostrongylus cantonensis (A. cantonensis) infection causes eosinophilic meningitis in humans. Eosinophilia and a Th2-type immune response are the crucial immune mechanisms for eosinophilic meningitis. CD4+CD25+ regulatory T cells (Treg) are involved in the pathogenesis of A. cantonensis. Diammonium glycyrrhizinate (DG) is a compound related to glycyrrhizin (GL), a triterpene glycoside extracted from liquorice root. We investigated the curative effects and probable mechanisms of therapy involving a combination of albendazole and DG in BALB/c mice infected with A. cantonensis, and compared these with therapy involving albendazole and dexamethasone. We analysed survival time, body weight, signs, eosinophil numbers, immunoglobulin E (IgE), interleukin-5 (IL-5), and eotaxin concentrations, numbers and Foxp3 expression of CD4+CD25+ Treg, worm recovery and histopathology. The present results demonstrated that the combination of albendazole and DG could increase survival time more efficiently and relieve neurological dysfunction; decrease weight loss, eosinophil numbers, concentrations of IgE, IL-5 and eotaxin, the number and expression of Foxp3 of CD4+CD25+ Treg; and improve worm recovery and histopathology changes in treated animals, compared with the combination of albendazole and dexamethasone. The observations presented here suggest that the albendazole and dexamethasone combination could be replaced by the combination of albendazole and DG.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 1 , March 2013 , pp. 1 - 11
Copyright
Copyright © Cambridge University Press 2011

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