Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-24T15:01:14.305Z Has data issue: false hasContentIssue false
  • English
  • Français

Absence of Helicobacter pylori in healthy laryngeal mucosa

Published online by Cambridge University Press:  01 November 2011

I Pajić-Penavić ,
D Đanić ,
S Maslovara  and
K Gall-Trošelj
I Pajić-Penavić*
Affiliation:
ENT Department, Dr Josip Benčević General Hospital, Slavonski Brod, Croatia
D Đanić
Affiliation:
ENT Department, Dr Josip Benčević General Hospital, Slavonski Brod, Croatia
S Maslovara
Affiliation:
ENT Department, Vukovar General Hospital, Zagreb, Croatia
K Gall-Trošelj
Affiliation:
Laboratory of Epigenomics, Ruđer Bošković Institute, Zagreb, Croatia
*
Address for correspondence: Dr Ivana Pajić-Penavić, Kneza Domagoja 4, 35 000 Slavonski Brod, Croatia Fax: +385 35 446 177 E-mail: ivana.pajic-penavic@sb.t-com.hr
Get access Rights & Permissions [Opens in a new window]

Abstract

Objectives:

To evaluate the presence of Helicobacter pylori in healthy laryngeal mucosa.

Design:

Prospective analysis of Helicobacter pylori colonisation in healthy laryngeal mucosa, using the 13C urea breath test and polymerase chain reaction analysis.

Subjects:

Twenty randomly chosen men (28–78 years) without laryngeal pathology or gastrointestinal disease were investigated. All subjects were scheduled for elective operative procedures, under general, endotracheal anaesthesia. Cytobrush samples were taken for Helicobacter pylori DNA detection. Nested polymerase chain reaction testing was performed on DNA solutions using two primer pairs from the urease A gene of the Helicobacter pylori genome. The 13C urea breath test was performed on two exhalation samples.

Results:

Eight (40 per cent) of the subjects were positive for urease on urea breath testing; none were positive for Helicobacter pylori DNA on polymerase chain reaction testing.

Conclusion:

Based on these results, we do not consider Helicobacter pylori to be a normal constituent of healthy laryngeal microflora.

Keywords

Helicobacter PyloriMicrobiologyBacteriologyLarynxPolymerase Chain Reaction
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 2 , February 2012 , pp. 196 - 199
Copyright
Copyright © JLO (1984) Limited 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Logan, RPH. C13 urea breath test. In: Lee, A, Megraud, F, eds. Helicobacter Pylori: Technique for Clinical Diagnosis and Basic Research. London: WB Saunders, 1996;74–82Google Scholar
2Breuer, T, Graham, DY. Can the urea-breath test be challenged by endoscopy as first line diagnostic tool? A cost effective analysis based on antibiotic resistance against initial treatment choice. Ann Intern Med 1995;123:260–8Google Scholar
3Osaki, T, Mabe, K, Hanawa, T, Ksmiya, S. Urease-positive bacteria in the stomach induce false-positive reaction in a urea breath test for diagnosis of Helicobacter pylori infection. J Med Microbiol 2008;57:814–19CrossRefGoogle Scholar
4Hua, J, Birac, C, Megraud, F. PCR based RAPD (random amplified polymorphic DNA) ‘fingerprinting’ of clinically isolated Helicobacter pylori. In: Lee, A, Megraud, F, eds. Helicobacter Pylori: Techniques for Clinical Diagnosis and Basic Research. London: WB Saunders, 1996;121–7Google Scholar
5Mravak-Stipetic, M, Gall-Trošelj, K, Lukac, J, Kusic, Z, Pavelic, K, Pavelic, J. Detection of Helicobacter pylori in various oral lesions by nested polymerase chain reaction (PCR). J Oral Pathol Med 1998;27:1–3Google Scholar
6Wang, JT, Lim, JT, Shen, JC, Yang, JC, Chen, DS, Wang, TH. Detection of Helicobacter pylori in gastric biopsy tissue by polymerase chain reaction. Eur J Clin Microbiol Infect Dis 1993;12:367–71CrossRefGoogle ScholarPubMed
7Kuipers, EJ, Thijs, JC, Festen, HP. The prevalence of Helicobacter pylori in peptic ulcer disease. Aliment Pharmacol Ther 1995;9(suppl 2):59–69Google ScholarPubMed
8Genta, RM, Gurer, IE, Graham, DY. Geographic pathology of Helicobacter pylori infection: is there more than one gastritis? Ann Med 1995;27:595–9CrossRefGoogle ScholarPubMed
9Hunt, RH. Eradication of Helicobater pylori infection. Am J Med 1996;20:100(5A) 42S–51SCrossRefGoogle Scholar
10Whincup, PH, Mendall, MA, Perry, IJ, Strachan, DP, Walker, M. Prospective relations between Helicobacter pylori infection, coronary heart disease and stroke in middle age men. Heart 1996;75:568–72CrossRefGoogle Scholar
11Rebora, A, Drago, F, Parodi, A. May Helicobacter pylori be important for dermatologists? Dermatology 1995;191:6–8Google Scholar
12Koc, C, Anikan Osman, K, Atasoy, P, Aksoy, K. Prevalence of Helicobacter pylori in patients with nasal polyposis: a preliminary report. Laryngoscope 2004;114:1941–1944Google Scholar
13Prelipcean, CC, Mihai, C, Gogắlniceanus, P, Mitricắ, D, Drug, VL, Stanciu, C. Extragastric manifestations of Helicobacter pylori infection. Rev Med Chir Soc Med Nat Iasi 2007;111:575–83Google Scholar
14Karczewska, E, Konturek, JE, Konturek, PC, Cześnikiewicz, M, Sito, E, Bielański, W et al. Oral cavity as a potential source of gastric reinfection by Helicobacter pylori. Dig Dis Sci 2002;47:978–86CrossRefGoogle ScholarPubMed
15Gall-Trošelj, K, Mravak-Stipetić, M, Jurak, I, Ragland, WL, Pavelić, J. Helicobacter pylori colonization of tongue mucosa – increased incidence in atrophic glossitis, glossopyrosis and burning mouth syndrome (BMS). J Oral Pathol Med 2001;30:560–3CrossRefGoogle Scholar
16Nguyen, AM, Engstrand, L, Genta, RM, Graham, DY, El-Zaatari, FA. Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. J Clin Microbiol 1993;31:783–7CrossRefGoogle ScholarPubMed
17Liu, Y, Yue, H, Li, A, Wang, J, Jiang, B, Zhang, Y et al. An epidemiologic study on the correlation between oral Helicobacter pylori and gastric H. pylori. Curr Microbiol 2009;58:449–53CrossRefGoogle ScholarPubMed
18Artiko, VM, Obradović, VB, Petrović, NS, Davidović, BM, Grujić-Adanja, GS, Nastić-Mirić, DR et al. 14C-urea breath test in detection of Helicobacter pylori infection. Nucl Med Rev 2001;4:101–3Google ScholarPubMed
19Borkowski, G, Sudhoff, H, Koslowski, F. A possible role of Helicobacter pylori infection in the etiology of chronic laryngitis. Eur Arch Otorhinolaryngol 1997;254:481–2Google Scholar
20Akbayir, N, Basak, T, Seven, H, Sungum, A, Erdem, L. Investigation of Helicobacter pylori colonisation in laryngeal neoplasia. Eur Arch Otorhinolaryngol 2005;262:170–2Google Scholar
21Fang, T-J, Lee, L-A, Li, H-Y, Yang, C, Huang, C-G. Helicobacter pylori colonization in the larynges of patients with hoarseness. Laryngoscope 2008;118:389–93CrossRefGoogle ScholarPubMed
22Rubin, JS, Benjamin, E, Prior, A, Lavy, J. The prevalence of Helicobacter pylori infection in malignant and premalignant conditions of head and neck. J Laryngol Otol 2003;117:118–21CrossRefGoogle ScholarPubMed
23Titiz, A, Ozcakir, O, Ceyhan, S, Yilmaz, YF, Unal, A, Akyon, Y. The presence of Helicobacter pylori in the larynx pathologies. Auris Nasus Larynx 2008;35:534–8Google Scholar
24Cvorovic, L, Brajovic, D, Strbac, M, Milutinovic, Z, Cvorovic, V. Detection of Helicobacter pylori in nasal polyps: preliminary report. J Otolaryngol Head Neck Surg 2008;37:192–5Google Scholar
25Zhuo, X, Wang, Y, Zhuo, W, Zhang, X. Possible association of Helicobacter pylori infection with laryngeal cancer risk: an evidence-based meta analysis. Arch Med Res 2008;39:625–8Google Scholar
26Grbesa, I, Marinkovic, M, Ivkic, M, Kruslin, B, Novak-Kujundzic, R, Pegan, B et al. Loss of imprinting of IGF2 and H19, loss of heterozygosity of IGF2R and CTCF, and Helicobacter pylori infection in laryngeal squamous cell carcinoma. J Mol Med 2008;86:1057–66Google Scholar