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Cultured stellate cells in human vocal fold mucosa

Published online by Cambridge University Press:  03 April 2008

K Sato ,
Y Miyajima ,
S Izumaru  and
T Nakashima
K Sato*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
Y Miyajima
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
S Izumaru
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
T Nakashima
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
*
Address for correspondence: Dr Kiminori Sato, Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan. Fax:  +81 942 37 1200 E-mail: kimisato@oct-net.ne.jp
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Abstract

Objectives:

Stellate cells in the maculae flavae, located at both ends of the human vocal fold mucosa, have been considered an independent category of cells. We aimed to isolate and subculture these stellate cells, and to observe their morphological characteristics.

Methods:

Stellate cells from the maculae flavae and fibroblasts from Reinke's space were cultured in three normal, adult human vocal fold mucosa preparations.

Results:

The subcultured cells from Reinke's space were conventional fibroblasts. The subcultured cells from the maculae flavae were stellate in shape and had cytoplasmic processes. They were larger than conventional fibroblasts, and lipid droplets in the cytoplasm disappeared in the second culture. These stellate cells proliferated by attaching their cytoplasmic processes to each other. During the seven to 10 month subculture period, each cell type continued to exhibit its own morphological characteristics.

Conclusion:

This study demonstrated that such stellate cells form an independent cell category, which should be considered as a new category of cells within the human vocal fold.

Keywords

LarynxVocal FoldsMorphologyStellate Cells
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 12 , December 2008 , pp. 1339 - 1342
Copyright
Copyright © JLO (1984) Limited 2008

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Footnotes

Presented at the 128th Annual Meeting of the American Laryngological Association, 26–27 April 2007, San Diego, California, USA.

References

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