Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-12T21:58:23.799Z Has data issue: false hasContentIssue false
  • English
  • Français

Cyclin D1 protein expression is related to clinical progression in laryngeal squamous cell carcinomas

Published online by Cambridge University Press:  29 June 2007

Pasquale Capaccio ,
Giancarlo Pruneri ,
Nadia Carboni ,
Angelo Virgilio Pagliari ,
Roberto Buffa ,
Antonino Neri ,
Antonio Ottaviani  and
Lorenzo Pignataro
Pasquale Capaccio
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Giancarlo Pruneri
Affiliation:
II Servizio di Anatomia Patologica, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Nadia Carboni
Affiliation:
II Servizio di Anatomia Patologica, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Angelo Virgilio Pagliari
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Roberto Buffa
Affiliation:
II Servizio di Anatomia Patologica, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Antonino Neri
Affiliation:
Servizio di Ematologia, Laboratorio di Ematologia Sperimentale e Genetica Molecolare, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Antonio Ottaviani
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Lorenzo Pignataro*
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
*
Address for correspondence: Lorenzo Pignataro, M.D., Clinica Otorinolaringoiatrica I, Ospedale Maggiore, I.R.C.C.S., Via F. Sforza 35, 20122, Milano, Italy. Fax: +39(2)55180809.
Get access Rights & Permissions [Opens in a new window]

Abstract

The expression of cyclin D1 gene was investigated in 74 laryngeal squamous cell carcinomas (LSCCs) in order to determine its clinical and prognostic value. Overexpression of cyclin Dl was detected immunohistochemically using DCS6 monoclonal antibody on formalin-fixed, paraffin-embedded tissue sections. Cyclin D1 expression was detected in 22 of the 74 cases investigated (30 per cent), thirteen of which presented nodal metastases (59 per cent); of the patients without any detectable cyclin D1 protein expression, six presented nodal metastases (12 per cent). Cyclin D1 protein expression was found in five per cent of the specimens of normal mucosa, eight per cent of thosewith low-grade dysplasia and 20 per cent of those with high-grade dysplasia. A statistically significant association was found between cyclin D1 expression and the supraglottic site (p<0.05), tumour extension (p<0.001), the presence of lymph node metastases (p<0.001), and advanced clinical stage (p<0.001). Cyclin D2 expression analysis is an important tool in the selection of LSCC patients with an aggressive clinical course.

Keywords

Carcinomasquamous cellLarynxTumour markersbiological
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 7 , July 1997 , pp. 622 - 626
Copyright
Copyright © JLO (1984) Limited 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adelaide, J., Mattei, M. G., Maries, I., Raybond, F., Planche, J., De Lapeyrieve, O., Bimbaum, D. (1988) Chromosomal localization of thehst oncogene and its co-amplification with the int-2 oncogene in a human melanoma. Oncogene 2: 413–416.Google Scholar
Armitage, P., Berry, G. (1991) Statistical Methods in Medical Research, Blackwell Scientific Publications, Oxford, pp 125–132.Google Scholar
Atula, T. S., Grénman, R., Varpula, M. J., Kurki, T. J. I., Klemi, P.-J. (1996) Palpation, ultrasound, and ultrasoundguided fine-needle aspiration cytology in the assessment of cervical lymph node status in head and neck cancer patients. Head and Neck Cancer 18:545–551.3.0.CO;2-2>CrossRefGoogle ScholarPubMed
Bartkova, J., Lukas, J., Muller, H., Lutzhoft, D., Strauss, M., Bartek, J. (1994) Cyclin Dl protein expression and function in human breast cancer. International Journal of Cancer 57: 353–361.CrossRefGoogle Scholar
Bartkova, J., Lukas, J., Strauss, M., Bartek, J. (1995a) Cyclin Dl oncoprotein aberrantly accumulates in malignancies of diverse histogenesis. Oncogene 10: 775–778.Google Scholar
Bartkova, J., Lukas, J., Muller, H., Strauss, M., Gusterson, B., Bartek, J. (1995b) Abnormal patterns of D-type cyclin expression and Gl regulation in human head and neck cancer. Cancer Research 55; 949–956.Google Scholar
Bellacosa, A., Almadori, G., Cavallo, S.,Cadoni, G., Galli, J., Ferrandina, G., Scambia, G., Neri, A. (1996) Cyclin Dl gene amplification in human laryngeal squamous cell carcinomas: prognostic significance and clinical implications. Clinical Cancer Research 2: 175–180.Google Scholar
Casey, G., Smith, R., McGillifray, D., Peters, G., Dickson, C. (1986) Characterization and chromosome assignment of the human homolog of int-2, a potential proto-oncogene. Molecular and Cellular Biology 6: 502–510.Google Scholar
Cattaruzza, M. S., Maisonneuve, P., Boyle, P. (1996) Epidemiology of laryngeal cancer. Oral Oncology, European Journal of Cancer 32B(5): 293–305.Google Scholar
Cordon-Cardo, C. (1995) Mutation of cell cycleregulators. Biological and clinical implications for human neoplasia. American Journal of Pathology 147: 545–560.Google Scholar
Courjal, F., Louason, G., Speiser, P., Katsaros, D., Zeillinger, R., Theillet, C. (1996) Cyclin Dl gene amplification and overexpression in breast and ovarian cancers: evidence for the selection of cyclin Dl in breast and cyclin E in ovarian tumors. International Journal of Cancer 69: 247–253.3.0.CO;2-X>CrossRefGoogle Scholar
Davidson, B. J., Lydiatt, W. M., Abate, M. P., Schantz, S. P., Chaganti, R. S. K. (1996) Cyclin Dl abnormalities and tobacco exposure in head and neck squamous cell carcinoma. Head and Neck 18: 512–521.Google Scholar
Field, J. K. (1992) Oncogenes and tumour-suppressor genes in squamous cell carcinoma of the head and neck. Oral Oncology, European Journal of Cancer 28B: 67–76.Google Scholar
Fracchiolla, N. S., Pignataro, L., Capaccio, P., Trecca, D., Boletni, A., Ottaviani, A., Polli, E., Maiolo, A. T., Neri, A. (1995) Multiple genetic lesions in laryngeal squamous cell carcinomas. Cancer 75: 1292–1301.Google Scholar
Gallo, O., Boddi, V., Bottai, G. V., Parrella, F., Fini Storchi, O. (1996) Treatment of the clinically negative neck in laryngeal cancer patients. Head and Neck 18: 566–572.3.0.CO;2-6>CrossRefGoogle ScholarPubMed
Gillett, C., Fantl, V., Smith, R., Fisher, C., Bartek, J., Dickson, C., Barnes, D., Peters, G. (1994) Amplification and overexpression of cyclin D in breast cancer detected by immunohistochemical staining. Cancer Research 54: 1812–1817.Google ScholarPubMed
Goldsmith, M. M., Pillsbury, H. C. (1991) Pathologic and cytometric indicators of biologic behaviour in laryngeal cancer. In Laryngeal Cancer. (Silver, C. E., ed.), Thieme Medical Publisher, New York, pp 27–32.Google Scholar
Hsu, S. M., Raine, L., Fanger, H. (1981) Use of avidin-biotinperoxidase complex (ABC): a comparisonbetween ABC and unlabelled antibody (PAP) procedures. Journal of Histochemistry and Cytochemistry 29: 577–580.Google Scholar
Jares, P., Fernandez, P. L., Campo, E., Nadal, A., Bosch, F., Aiza, G., Nayach, I., Traserra, J., Cardesa, A. (1994) PRAD-1/Cyclin Dl gene amplification correlates with messenger RNA overexpression and tumour progression in human laryngeal carcinomas. Cancer Research 54: 4813–4817.Google Scholar
Johnson, N. W. (1990) Oro-facial Neoplasms: Global Epidemiology, Risk Factors and Recommendations for Research. Federation Dentaire Internationale, FDI Technical report, London, pp 36.Google Scholar
Leonard, J. H., Kearsley, J. H., Chenevix-Trench, G., Hayward, N. K. (1991) Analysis of gene amplification in head and neck squamous cell carcinomas. International Journal of Cancer 48: 511–515.Google Scholar
Li, X. M., Wei, W. I., Guo, X. F., Yuen, P. W., Lam, L. K. (1996) Cervical lymph node metastatic patterns of squamous carcinomas in the upper aerodigestive tract. Journal of Laryngology and Otology 110: 937–941.CrossRefGoogle ScholarPubMed
Michalides, R., van Veelen, N., Hart, A.,Loftus, B., Wientjens, E., Balm, A. (1995) Overexpression of cyclin Dl correlates with recurrence in a group of forty-seven operable squamous cell carcinomas of the head and neck. Cancer Research 55: 975–978.Google Scholar
Michalides, R., Hageman, P., van Tinteren, H., Houben, L., Wientjens, E., Klompmaker, R., Peterse, J. (1996) A cinicopathological study on overexpression of cyclin Dl and of p53 in a series of 248 patientswith operable breast cancer. British Journal of Cancer 73: 728–734.Google Scholar
Motokura, T., Bloom, T., Kim, H. G., Juppner, H., Ruderman, J. V., Kronenberg, H. M., Arnold, A. (1991) A novel cyclin encoded by a bcll -linked candidate oncogene. Nature 350: 512–515.Google Scholar
Muller, D., Millon, R., Lidereau, R., Engelmann, A., Bronner, G., Flesch, H., Eber, M., Methlin, G., Abecassis, J. (1994) Frequent amplification of Ilql3 DNA markers is associated with lymph node involvement in human head and neck squamous cell carcinomas. Oral Oncology, European Journal of Cancer 30B: 113–120.Google Scholar
Patel, A. M., Incognito, L. S., Schecter, G. L., Wasilenko, W. J., Somers, K. D. (1996)Amplification and expression of EMS-1 (cortactin) in head and neck squamous cell carcinoma cell lines. Oncogene 12: 31–35.Google Scholar
ignataro, L., Capaccio, P., Pruneri, G., Carboni, N., Fracchiolla, N. S., Richelda, R., Scaramellini, G., Neri, A., Buffa, R. (1996) p53 and cyclin Dl protein expression in glottic laryngeal squamous cell carcinomas involving the anterior commissure (pTlbN0M0). International Journal of Oncology 9: 553–557.Google Scholar
Rosenberg, C. L., Wong, E., Petty, E. M.,Bale, A. E., Tsujimoto, Y., Harris, N. L., Arnold, A. (1991) PRAD1, a candidate BCL1 oncogene: mapping and expression in centrocytic lymphoma. Proceedings of the National Academy of Sciences of USA (Wash) 88: 9638–9642.CrossRefGoogle ScholarPubMed
Schuuring, E., Verhoeven, E., van Tinteren, H., Peterse, J. L., Nunnink, B., Thunnissen, F. B. J. M., Devilee, P., Cornelisse, C. J., van de Vijver, M. J., Mooi, W. J., Michalides, R. J. A. M. (1992) Amplification of genes within the chromosome 1lql3 region is indicative of poor prognosis in patients with operable breast cancer. Cancer Research 52: 5229–5234.Google Scholar
Shanmugaratnam, K., Sobin, I. N., and pathologists in eight countries (1991) Histological typing of tumours of the upper respiratory tract and ear. WHO Geneva.Google Scholar
Shinozaki, H., Ozawa, S., Ando, N., Tsuruta, H., Terada, M., Ueda, M., Kitajima, M. (1996) Cyclin Dl amplification as a new predictive classification for squamous cell carcinoma of the esophagus, adding gene information. Clinical Cancer Research 2: 1155–1161.Google Scholar
UICC (1987) TNM Classification of Malignant Tumors. (Hermanek, P., Sobin, L. H., eds.), Springer Verlag, Berlin.Google Scholar
Wang, T. C., Cardiff, R. D., Zukerberg, L., Lees, E., Arnold, A., Schmidt, E. V. (1994) Mammary hyperplasia and carcinoma in MMTV-cyclin Dl transgenic mice. Nature 369: 669–671.Google Scholar
Zhang, Y. J., Jiang, W., Chen, C. J., Lee, C. S., Kahn, S. M., Santella, R. M., Weinstein, I. B. (1993) Amplification and overexpressionof Cyclin Dl in human hepatocellular carcinoma. Biochemical Biophysical Research Communications 196: 1010–1016.Google Scholar