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Hearing outcome of early postnatal exposure to hypoxia in Sprague–Dawley rats

Published online by Cambridge University Press:  15 April 2014

S J Daniel ,
M McIntosh ,
O V Akinpelu  and
C V Rohlicek
S J Daniel*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada McGill Auditory Sciences Laboratory, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada
M McIntosh
Affiliation:
Division of Cardiology, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada
O V Akinpelu
Affiliation:
McGill Auditory Sciences Laboratory, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada
C V Rohlicek
Affiliation:
Division of Cardiology, Montreal Children's Hospital – McGill University Health Centre, Quebec, Canada Department of Physiology, McGill University, Montreal, Quebec, Canada
*
Address for correspondence: Dr S J Daniel, Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal Children's Hospital, B240, 2300 Tupper Avenue, Montreal, Quebec H3H 1P3, Canada Fax: 514-412-4342 E-mail: sam.daniel@mcgill.ca
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Abstract

Objective:

To determine the effect of experimentally induced hypoxia, in the first 10 days of life, on physiological hearing in a Sprague–Dawley rat model.

Methods:

A prospective, controlled animal study was carried out using 22 male rat pups. The rats in the hypoxic group (n = 12) were reared in hypoxia for the first 10 days of life, and subsequently reared in normoxia, while those in the control group (n = 10) were reared in normoxia for the duration of the experiment. Hearing was assessed using auditory brainstem response testing at approximately 72 days of age.

Results:

The hypoxia group had higher auditory brainstem response thresholds for all frequencies tested (more pronounced at 16 kHz), compared with controls. Wave I–V inter-peak latencies were more prolonged in the hypoxic rats, while both groups had similar wave I latencies.

Conclusion:

Chronic postnatal hypoxia induced permanent hearing loss in this Sprague–Dawley rat model. Prolonged wave I–V inter-peak latencies suggested functional abnormality in the central auditory pathway.

Keywords

HypoxiaAnimals, NeonatalRats, Sprague-DawleyCochleaEvoked PotentialsCentral Auditory Pathway DisordersAuditory Brainstem Responses
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 128 , Issue 4 , April 2014 , pp. 331 - 335
Copyright
Copyright © JLO (1984) Limited 2014 

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References

1Rothman, SM, Olney, JW. Glutamate and the pathophysiology of hypoxic-ischemic brain damage. Ann Neurol 1986;19:105–11Google Scholar
2Puschner, B, Schacht, J. Energy metabolism in cochlear outer hair cells in vitro. Hear Res 1997;114:102–6CrossRefGoogle ScholarPubMed
3Lipton, P. Ischemic cell death in brain neurons. Physiol Rev 1999;79:1431–568Google Scholar
4Mazurek, B, Winter, E, Fuchs, J, Haupt, H, Gross, J. Susceptibility of the hair cells of the newborn rat cochlea to hypoxia and ischemia. Hear Res 2003;182:2–8Google Scholar
5Shirane, M, Harrison, RV. The effects of hypoxia on sensory cells of the cochlea in the chinchilla. Scanning Microsc 1987;1:1175–83Google ScholarPubMed
6Ide, R, Harada, T, Kanzaki, S, Saito, H, Hoshikawa, M, Kawahara, T et al. Physical and physiological effects on otoacoustic emissions in hypobaric hypoxia. ORL J Otorhinolaryngol Relat Spec 2010;72:225–32Google Scholar
7Zang, Z, Wilkinson, AR, Jiang, ZD. Distortion product otoacoustic emissions at 6 months in term infants after prenatal hypoxia-ischaemia or with low Apgar score. Eur J Pediatr 2008;167:575–8Google Scholar
8Jiang, ZD, Yin, R, Shao, XM, Wilkinson, AR. Brain-stem auditory impairment during the neonatal period in term infants after asphyxia: dynamic changes in brain-stem auditory evoked response to clicks of different rates. Clin Neurophysiol 2004;115:1605–15Google Scholar
9Sohmer, H, Gafni, M, Chisin, R. Auditory nerve-brain stem potentials in man and cat under hypoxic and hypercapnic conditions. Electroencephalogr Clin Neurophysiol 1982;53:506–12Google Scholar
10Attias, J, Sohmer, H, Gold, S, Haran, I, Shahar, A. Noise and hypoxia induced temporary threshold shifts in rats studied by ABR. Hear Res 1990;45:247–52CrossRefGoogle ScholarPubMed
11Birnholz, JC, Benacerraf, BR. The development of human fetal hearing. Science 1983;222:516–18CrossRefGoogle ScholarPubMed
12Romand, R, Despres, G, Giry, M. Factors affecting the onset of inner ear function. Hear Res 1987;28:1–7CrossRefGoogle ScholarPubMed
13Cycowicz, Y, Schmuel, M, Freeman, S, Wanszelbaum, A, Sohmer, H. Perinatal hypoxia and auditory brainstem response thresholds: no evidence of permanent hearing loss. Hear Res 1988;33:239–44Google Scholar
14Gafni, M, Sohmer, H. Intermediate endocochlear potential levels induced by hypoxia. Acta Otolaryngol 1976;82:345–8CrossRefGoogle ScholarPubMed
15Geal-Dor, M, Freeman, S, Li, G, Sohmer, H. Development of hearing in neonatal rats: air and bone conducted ABR thresholds. Hear Res 1993;69:236–42Google Scholar
16Kusakari, J, Kambayashi, J, Kobayashi, T, Rokugo, M, Kawamoto, M. Reversibility of the endolymphatic potential after transient anoxia. Tohoku J Exp Med 1980;131:1–5CrossRefGoogle ScholarPubMed
17Pierson, MG, Moller, AR. Corresponding effects of hypoxia on the cochlear microphonic and the compound action potential. Hear Res 1982;6:83–101CrossRefGoogle ScholarPubMed
18Koga, K, Hakuba, N, Watanabe, F, Shudou, M, Nakagawa, T, Gyo, K. Transient cochlear ischemia causes delayed cell death in the organ of Corti: an experimental study in gerbils. J Comp Neurol 2003;456:105–11Google Scholar
19Khan, M, Szczepek, AJ, Haupt, H, Olze, H, Mazurek, B. Expression of pro-inflammatory cytokines in cochlear explant cultures: influence of normoxia and hypoxia. Neurosci Lett 2010;479:249–52Google Scholar