Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-18T02:57:57.141Z Has data issue: false hasContentIssue false
  • English
  • Français

The localization and specificity of guinea pig inner ear antigenic epitopes

Published online by Cambridge University Press:  29 June 2007

Ming-Yu Cao ,
Michel Gersdorff ,
Naïma Deggouj  and
Jean-Paul Tomasi
Ming-Yu Cao*
Affiliation:
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium. Department of Laboratory of Autoimmunity, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
Michel Gersdorff
Affiliation:
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
Naïma Deggouj
Affiliation:
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
Jean-Paul Tomasi
Affiliation:
Department of Laboratory of Autoimmunity, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
*
Address for correspondence: Dr Ming-Yu Cao, Laboratoire d'Auto-immunité, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Ave. Hippocrate 53, 1200 Brussels, Belgium. Fax: +32-2-76 9428.
Get access Rights & Permissions [Opens in a new window]

Abstract

In this study, we investigated the relative localization of some antigenic epitopes in the inner ear. The inner ear protein antigens were extracted from various parts of the guinea pig inner ear. Brain, kidney, lung, heart and liver extracts were also obtained. We found by SDS-polyacrylamide gel electrophoresis that total inner ear extracts separated into three high concentration polypeptide bands with molecular weights of approximately 30, 42, 58 kd and three low density bands of 20,25 and 35 kd. The 30 kd band was found mainly in the extract of the spiral ganglion and the acoustic nerve in the modiolus. The 42 and 58 kd bands were detected in the extract of the spiral ligament and the stria vascularis. The Organ of Corti and the basilar membrane extract gave rise to three bands of 30,42 and 58 kd. Twenty-eight of the 75 sera from patients with inner ear disease reacted with the 30 and 58 kd bands of the inner ear protein extracts by immunoblotting. Sixteen of these 28 positive sera were then used to probe immunoblots of the brain, kidney, lung, heart and liver extracts. The 58 kd band was also found in protein extracts of the brain, the lung and the liver. This study suggests that the 30 kd antigenic epitope may be mainly related to the acoustic nerve and that the 58 kd antigenic epitope is not cochlear specific.

Keywords

CochleaHearing loss, SensorineuralAutoantibodiesImmunoblotting
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 1 , January 1995 , pp. 19 - 23
Copyright
Copyright © JLO (1984) Limited 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Presented at the International Academic Conference oflmmunobiology in Otology, Rhinology and Laryngology in Oita, Japan, April 4–7 1994.

References

Arnold, W., Pfaltz, R., Altermatt, H.-J. (1985) Evidence of serum antibodies against inner ear tissues in the blood of patients with certain sensorineural hearing disorders. Acta Otolaryngologica 99: 437–444.CrossRefGoogle ScholarPubMed
Cao, M. Y., Wong, Y., Wang, J., Dai, S. H. (1988) Scanning electron microscope specimen preparation of guinea pig vestibular organ. Acta Academiae Medicinae Nanjing 8(4): 341–342.Google Scholar
Gersdorff, M, Cao, M.Y., Tomasi, J. -P., Deggouj, N. (1994) Maladies auto-immunes de l'oreille. Etude des auto-anticorps anticochlee. Rapport preliminaire. Revue de Laryngologie, Otologie, Rhinologie 115: 163–167.Google Scholar
Harris, J. P. (1983) Immunology of the inner ear: response of the inner ear to antigen challenge. Archives ofOtolaryngologw Head and Neck Surgery 91: 18–23.CrossRefGoogle ScholarPubMed
Harris, J. P. (1984) Immunology of the inner ear: evidence of local antibody production. Annals of Otology, Rhinologx and Laryngology 93: 157–162.CrossRefGoogle ScholarPubMed
Harris, J. P. (1987) Experimental autoimmune sensorineural hearing loss. Laryngoscope 97: 63–76.CrossRefGoogle ScholarPubMed
Harris, J. P., Sharp, P. A. (1990) Inner ear autoantibodies in patients with rapidly progressive sensorineural hearing loss. Laryngoscope 100: 516–524.CrossRefGoogle ScholarPubMed
Hughes, G. B., Kinney, S. E., Barna, B. P., Calabrese, L. H. (1984) Practical versus theoretical management of autoimmune inner ear disease. Laryngoscope 94: 758–766.CrossRefGoogle ScholarPubMed
Joliat, T., Seyer, J., Bernstein, J., Krug, M., Ye, X. J., Cho, J. S., Fujiyoshi, T., Yoo, T. J. (1992) Antibodies against a 30 kilodalton cochlear protein and type II and IX collagens in the serum of patients with inner ear disease. Annals of Otology, Rhinology and Laryngology 101: 1000–1006.CrossRefGoogle Scholar
Lejeune, J. M., Charachon, R. (1992) New immunobiological tests in the investigation of Meniere's disease and sensorineural hearing loss. Acta Otolaryngologica 112: 174–179.CrossRefGoogle ScholarPubMed
McCabe, B. F. (1979) Autoimmune sensorineural hearing loss. Annals of Otology, Rhinology and Laryngology 88: 585–590.CrossRefGoogle ScholarPubMed
Mogi, G., Lim, D. j., Watanabe, N. (1982) Immunologic study on the inner ear: immunoglobulins in perilymph. Archives of Otolaryngology 108: 270–275.CrossRefGoogle Scholar
Orozco, C. R., Niparko, J. K., Richardson, B. C, Dolan, D. E, Ptok, M. U., Altschuler, R. A. (1990) Experimental model of immunemedicated hearing loss using cross-species immunization. Laryngoscope 100: 941–947.CrossRefGoogle Scholar
Soliman, A. M. (1989) Experimental autoimmune inner ear disease. Laryngoscope 99: 188–193.CrossRefGoogle ScholarPubMed
Tomiyama, S., Harris, J. P. (1987) The role of the endolymphatic sac in inner ear immunity. Acta Otolaryngologica 103: 182–188.CrossRefGoogle ScholarPubMed
Veldman, J. E., Hanada, T., Meeuwsen, F. (1993) Diagnostic and therapeutic dilemmas in rapidly progressive sensorineural hearing loss and sudden deafness. Acta Otolaryngologica 113: 303–306.CrossRefGoogle ScholarPubMed
Yamanobe, S., Harris, J. P. (1992) Spontaneous remission experimental autoimmune labyrinthitis. Annals of Otology, Rhinology and Laryngology 101: 1007–1014.CrossRefGoogle ScholarPubMed
Yoo, T. J. (1984) Etiopathogenesis of otosclerosis: a hypothesis. Annals of Otology, Rhinology and Laryngology 93(1): 28–33.CrossRefGoogle ScholarPubMed
Yoo, T. J., Yazawa, Y, Floyd, R. (1983) Type II collagen-induced autoimmune endolymphatic hydrops in guinea pig. Science 222: 65–67.CrossRefGoogle ScholarPubMed
Yoo, T. J., Tomodo, K., Hernandez, A. D. (1984) Type II collageninduced autoimmune inner ear lesions in guinea pigs. Annals of Otology, Rhinology and Laryngology 93 (suppl. 113): 3–5.CrossRefGoogle Scholar