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Preparation of micro/nanometer-sized porous surface structure of calcium phosphate scaffolds and the influence on biocompatibility

Published online by Cambridge University Press:  22 May 2014

Chengde Gao
Affiliation:
State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha 410083, People's Republic of China
Jingyu Zhuang
Affiliation:
State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha 410083, People's Republic of China
Pengjian Li
Affiliation:
State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha 410083, People's Republic of China
Cijun Shuai*
Affiliation:
State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha 410083, People's Republic of China; and Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston 29425, South Carolina
Shuping Peng*
Affiliation:
Cancer Research Institute, Central South University, Changsha 410078, People's Republic of China
*
a)Address all correspondence to these authors. e-mail: shuai@csu.edu.cn
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Abstract

Multilayer stereo micro/nanometer-sized porous surface structures were prepared by selective chemical etching of biphasic calcium phosphate (BCP) scaffolds with hydroxyapatite (HAP)/β-tricalcium phosphate (β-TCP) weight ratios of 90/10, 80/20, 70/30, 60/40, and 50/50 in phosphoric acid solution. The porous surface structures revealed periodic fluctuations in the observed heights of micro/nanometer-sized needles. And the average height increased from 0.59 ± 0.02 to 12.09 ± 0.03 μm when the β-TCP content in BCP scaffolds rose from 10 to 50%. In vivo cell tests using MG-63 cells (belonging to the human osteosarcoma cell line) revealed that micro/nanometer-sized pores on the scaffold surface could provide location for cell adhesion and migration and facilitate the formation of gap junction between cells. The BCP scaffold with 40% β-TCP exhibited the optimal surface structure for cell seeding and growth due to the largest number of micro/nanometer-sized pores on the surface. However, excessive β-TCP led to the damage of micro/nanometer-sized porous surface structure, which further impeded the cell interaction.

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Articles
Copyright
Copyright © Materials Research Society 2014 

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References

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