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Quantitative and qualitative analysis of sea bream, Sparus aurata (L.), humoral immune response, vaccinated with commercial and experimental vaccines against vibriosis and photobacteriosis

Published online by Cambridge University Press:  13 September 2017

Vasileios Bakopoulos ,
Ioanna Kosma  and
Evita Laspa
Vasileios Bakopoulos*
Affiliation:
Department of Marine Sciences, School of The Environment, University of The Aegean, Lesvos, Greece
Ioanna Kosma
Affiliation:
Department of Marine Sciences, School of The Environment, University of The Aegean, Lesvos, Greece
Evita Laspa
Affiliation:
Department of Marine Sciences, School of The Environment, University of The Aegean, Lesvos, Greece
*
Correspondence should be addressed to: V. Bakopoulos Department of Marine Sciences, School of The Environment, University of The Aegean, University Hill, Mytilene 81100, Lesvos, Greece email: v.bakopoulos@marine.aegean.gr
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Abstract

The specific humoral immune response of sea bream, Sparus aurata (L.), against Vibrio anguillarum O1 and Photobacterium damselae subsp. piscicida (Phdp) after immunization with commercial and experimental bacterins was analysed quantitatively and qualitatively. Specific anti-V. anguillarum O1 and anti-Phdp levels provoked by the adjuvanted commercial vaccine reached higher levels in comparison to the aqueous commercial and experimental bacterins. Infection of vaccinated fish with V. anguillarum O1 bacterial cells acted as a boost of the humoral immune response, except for the sera of the group vaccinated with the adjuvanted vaccine. Infection with Phdp acted as a boost of the humoral immune response mainly for the group vaccinated with a monovalent Phdp bacterin and to a lesser degree for the group vaccinated with the aqueous commercial vaccine. Western blot analysis of the sera against V. anguillarum O1 whole cell antigens revealed strong reactions to only a few antigens below 54 kD and above 15 kD and weak reactions to other antigens. Similar reactions were observed from the sera isolated from the controls. Western blot analysis of the sera against Phdp whole cell antigens revealed strong reactions to only a handful of antigens below 20.7 and below 6.4 kD. Sera from the control group, as in the case of V. anguillarum O1, reacted with Phdp whole cell antigens. No differences regarding antigen reactions between monovalent and bivalent formulations were noted, in contrast to the adjuvanted and aqueous bacterins.

Keywords

Vibrio anguillarum O1Photobacterium damselae subsp. piscicidasea breamhumoral immune responsevaccines
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 98 , Special Issue 1: Special Section: European Marine Biology Symposium Papers 2016 , February 2018 , pp. 105 - 115
Copyright
Copyright © Marine Biological Association of the United Kingdom 2017 

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