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Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

Published online by Cambridge University Press:  09 June 2016

Thomas J. Ashton ,
Meriem Kayoueche-Reeve ,
Andrew J. Blight ,
Jon Moore  and
David M. Paterson
Thomas J. Ashton*
Affiliation:
Xelect Ltd, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
Meriem Kayoueche-Reeve
Affiliation:
The Sullom Voe Association Ltd, Shetland Oil Terminal Environmental Advisory Group (SOTEAG) Office, School of Biology, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
Andrew J. Blight
Affiliation:
Sediment Ecology Research Group, School of Biology, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
Jon Moore
Affiliation:
Aquatic Survey & Monitoring Ltd, Cosheston, Pembroke Dock, Pembrokeshire SA72 4UN, Wales, UK
David M. Paterson
Affiliation:
Sediment Ecology Research Group, School of Biology, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
*
Correspondence should be addressed to: T.J. Ashton, Xelect Ltd, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK email: tom.ashton@xelect.co.uk
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Abstract

Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.

Keywords

PatellalimpetsDNA barcodingmucusspecies determination
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 7 , November 2017 , pp. 1479 - 1482
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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References

REFERENCES

Cabral, J.P. (2003) Characterization and multivariate analysis of Patella intermedia, Patella ulyssiponensis and Patella vulgata from Povoa de Varzim (Northwest Portugal). Iberus 21, 1–17. http://biostor.org/reference/133321.Google Scholar
Delany, J., Myers, A.A. and McGrath, D. (1998) Recruitment, immigration and population structure of two coexisting limpet species in mid-shore tidepools, on the west coast of Ireland. Journal of Experimental Marine Biology and Ecology 221, 221–30.Google Scholar
Fish, J.D. and Fish, S. (2011) A students guide to the seashore, 3rd edition. Cambridge: Cambridge University Press, pp. 205–206.Google Scholar
Hajibabaei, M., Shokralla, S., Zhou, X., Singer, G.A.C. and Baird, D.J. (2011) Environmental barcoding: a next-generation sequencing approach for biomonitoring applications using river benthos. PLoS ONE 6, e17497.Google Scholar
Hayward, P.J. and Ryland, J.S. (1995) Handbook of the marine fauna of North-West Europe. Oxford: Oxford University Press.Google Scholar
Hebert, P.D.N., Cywinska, A., Ball, S.L. and DeWaard, J.L. (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society (Biological Sciences Series) 270, 313–321.Google Scholar
Hebert, P.D.N., Ratnasingham, S. and DeWaard, J.L. (2003b) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society (Biological Sciences Series) 270, S96–S99.Google Scholar
Hollingsworth, P.M., Forrest, L.L., Spouge, J.L., Hajibabaei, M., Ratnasingham, S. and Little, D.P. (2009) A DNA barcode for land plants. Proceedings of the National Academy of Sciences USA 106, 12794–12797.Google Scholar
Kawai, K., Shimizu, M., Hughes, R.N. and Takenaka, O. (2004) A non-invasive technique for obtaining DNA from marine intertidal snails. Journal of the Marine Biological Association of the United Kingdom 66, 773–774.Google Scholar
Kress, W.J., García-Robledo, C., Uriarte, M. and Erickson, D.L. (2015) DNA barcodes for ecology, evolution and conservation. Trends in Ecology and Evolution 30, 25–35.Google Scholar
Le Vin, A.L., Adam, A., Tedder, A., Arnold, K.E. and Mable, B.K. (2011) Validation of swabs as a non-destructive and relatively non-invasive DNA sampling method in fish. Molecular Ecology Resources 11, 107–109.Google Scholar
Little, C. and Kitching, J.A. (1996) The biology of rocky shores. Oxford: Oxford University Press.Google Scholar
Livia, L., Antonella, P., Hovirag, L., Mauro, N. and Panara, F. (2006) A nondestructive, rapid, reliable and inexpensive method to sample, store and extract high-quality DNA from fish body mucus and buccal cells. Molecular Ecology Notes 6, 257–260.Google Scholar
Moore, H.B. (1934) The relation of shell growth to environment in Patella vulgata . Journal of Molluscan Studies 21, 217–222.Google Scholar
Moore, J. and Howson, C. (2012) Survey of the rocky shores in the region of Sullom Voe, Shetland, July 2012. A report to SOTEAG from Aquatic Survey & Monitoring Ltd, Cosheston, Pembrokeshire. 29 pp + v.Google Scholar
Moura, C.J., Harris, D.J., Cunha, M.R. and Rogers, A.D. (2007) DNA barcoding reveals cryptic diversity in marine hydroids (Cnidaria, Hydrozoa) from coastal and deep-sea environments. Zoologica Scripta 37, 93–108.CrossRefGoogle Scholar
Mrowicki, R.J., Maggs, C.A. and O'Connor, N.E. (2014) Does wave exposure determine the interactive effects of losing key grazers and ecosystem engineers? Journal of Experimental Marine Biology and Ecology 461, 416–424.Google Scholar
Thomas, A.C., Deagle, B.E., Eveson, J.P., Harsch, C.H. and Trites, A.W. (2016) Quantitative DNA metabarcoding: improved estimates of species proportional biomass using correction factors derived from control material. Molecular Ecology Resources 16, 714–726.Google Scholar
Valentini, A., Pompanon, F. and Taberlet, P. (2009) DNA barcoding for ecologists. Trends in Ecology and Evolution 24, 110–117.CrossRefGoogle ScholarPubMed