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First mitochondrial genome of a lugworm (Annelida: Arenicolidae) and its phylogenetic position

Published online by Cambridge University Press:  06 January 2023

Genki Kobayashi Open the ORCID record for Genki Kobayashi [Opens in a new window] ,
Hajime Itoh  and
Nobuyoshi Nakajima Open the ORCID record for Nobuyoshi Nakajima [Opens in a new window]
Genki Kobayashi*
Affiliation:
Ishinomaki Senshu University, 1 Shinmito Minamisakai, Ishinomaki, Miyagi 986-8580, Japan
Hajime Itoh
Affiliation:
National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Nobuyoshi Nakajima
Affiliation:
National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, Ibaraki 305-8506, Japan
*
Author for correspondence: Genki Kobayashi, E-mail: genkikobayashi5884@gmail.com
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Abstract

The annelid mitochondrial genomes (mitogenomes) have been well documented, and phylogenetic analyses based on the mitogenomes provide insightful implications for annelid evolution. However, the mitogenomes of some families remain unknown. Herein, we determined the complete mitogenome of the lugworm Abarenicola claparedi oceanica (15,524 bp), representing the first mitogenome from the family Arenicolidae. The gene order of this species is the same as the various lineages in Sedentaria. The maximum likelihood phylogenetic analyses were performed based on six different datasets, including 43 ingroups (oligochaetes, hirudineans, echiurans and closely related polychaetes) and two outgroups (Siboglinidae), namely, aligned and trimmed datasets consisting of the nucleotide sequences of protein-coding genes (PCGs) and rRNAs, and amino acid sequences of PCGs. Phylogenetic analyses based on the nucleotide sequences yielded trees with better support values than those based on the amino acid sequences. Arenicolidae is clustered with Maldanidae in all analyses. Analyses based on nucleotide sequences confirm the monophyly of Terebellidae, which was paraphyletic in recent mitogenomic phylogenetic studies. We also performed the phylogenetic analysis based on the RY-coding of the nucleotide sequences of PCGs only to yield phylogeny with generally low support values. Additional mitogenome sequences of related ingroup species would be needed to comprehensively understand the phylogenetic relationship, which was not present in this study.

Keywords

Arenicolidaintertidal zoneMaldanomorphamitogenomepolychaetes
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 102 , Issue 8 , December 2022 , pp. 635 - 644
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Marine Biological Association of the United Kingdom

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