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Development and characterization of fungal specific microsatellite markers in the lichen Lobarina scrobiculata (Lobariaceae, Ascomycota)

Published online by Cambridge University Press:  19 May 2015

Maria Prieto ,
Lidia Romera ,
Sonia Merinero ,
Gregorio Aragón  and
Isabel Martínez
Maria Prieto
Affiliation:
Departamento de Biología y Geología, Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. Email: maria.prieto@urjc.es
Lidia Romera
Affiliation:
Departamento de Biología y Geología, Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. Email: maria.prieto@urjc.es
Sonia Merinero
Affiliation:
Departamento de Biología y Geología, Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. Email: maria.prieto@urjc.es
Gregorio Aragón
Affiliation:
Departamento de Biología y Geología, Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. Email: maria.prieto@urjc.es
Isabel Martínez
Affiliation:
Departamento de Biología y Geología, Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. Email: maria.prieto@urjc.es
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Abstract

Lobarina scrobiculata (better known as Lobaria scrobiculata) is a widespread lichen, threatened and Red-Listed in various European countries. Microsatellite markers for the mycobiont of L. scrobiculata were developed in order to investigate its genetic diversity in the Iberian Peninsula and Europe and to design effective conservation strategies. A total of 7 polymorphic markers were isolated and characterized. These microsatellites were tested in natural populations found in the Iberian Peninsula. The number of observed alleles ranged from 3 to 8, and the Nei's unbiased gene diversity from 0·26 to 0·59. These microsatellite markers are the first to be developed for L. scrobiculata and they will be useful for population studies and for the assessment of the conservation status of this species.

Keywords

conservation statuscyanolichengenetic diversityLobaria scrobiculataSSR
Type
Articles
Information
The Lichenologist , Volume 47 , Issue 3 , May 2015 , pp. 183 - 186
Copyright
© British Lichen Society, 2015 

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References

Burgaz, A. R. Martínez, I. (1999) La familia Lobariaceae en la Península Ibérica. Botanica Complutensis 23: 59–90.Google Scholar
Burgaz, A. R., Fuertes, E. Escudero, A. (1994) Climax epiphytic communities in Mediterranean Spain. Botanical Journal of the Linnean Society 115: 35–47.Google Scholar
Clerc, P., Scheidegger, C. Ammann, K. (1992) Liste rouge des macrolichens de la Suisse. Botanica Helvetica 102: 71–83.Google Scholar
Gärdenfors, U. (2010) The 2010 Red List of Swedish Species. Uppsala: SLU.Google Scholar
Hallingbäck, T. (1989) Occurrence and ecology of the lichen Lobaria scrobiculata in southern Sweden. Lichenologist 21: 331–341.Google Scholar
James, P. W., Hawksworth, D. L. Rose, F. (1977) Lichen communities in the British Isles: a preliminary conspectus. In Lichen Ecology (M. R. D. Seaward, ed.): 295–413. London: Academic Press.Google Scholar
Mansournia, M. R., Wu, B., Matsushita, N. Hogetsu, T. (2012) Genotypic analysis of the foliose lichen Parmotrema tinctorum using microsatellite markers: association of mycobiont and photobiont, and their reproductive modes. Lichenologist 44: 419–440.CrossRefGoogle Scholar
Martínez, I., Aragón, G., Sarrión, F. J., Escudero, A., Burgaz, A. R. Coppins, B. J. (2003) Threatened lichens in central Spain (saxicolous species excluded). Cryptogamie Mycologie 24: 73–97.Google Scholar
Meglécz, E., Costedoat, C., Dubut, V., Gilles, A., Malausa, T., Pech, N. Martin, J. F. (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26: 403–404.Google Scholar
Merinero, S., Rubio-Salcedo, M., Aragón, G. Martínez, I. (2014) Environmental factors that drive the distribution and abundance of a threatened cyanolichen in Southern Europe: a multi-scale approach. American Journal of Botany 101: 1876–1885.Google Scholar
Moncada, B., Lücking, R. Betancourt-Macuase, L. (2013) Phylogeny of the Lobariaceae (lichenized Ascomycota: Peltigerales), with a reappraisal of the genus Lobariella . Lichenologist 45: 203–263.Google Scholar
Nimis, P. L. (1993) The Lichens of Italy. Torino: Museo Regionale di Scienze Naturali.Google Scholar
Otálora, M. G., Martínez, I., Belinchón, R., Widmer, I., Aragón, G., Escudero, A. Scheidegger, C. (2011) Remnants fragments preserve genetic diversity of the old forest lichen Lobaria pulmonaria in a fragmented Mediterranean mountain forest. Biodiversity and Conservation 20: 1239–1254.Google Scholar
Peakall, R. Smouse, P. E. (2006) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.CrossRefGoogle Scholar
Pentecost, A. Richardson, C. B. (2011) Niche separation and overlap in the foliose lichens Lobaria pulmonaria (L.) Hoffm. and L. virens (With.) Laundon in the Killarney Oak Woods, Ireland. Biology and Environment: Proceedings of the Royal Irish Academy 111B: 61–67.Google Scholar
Prieto, M., Martínez, I., Aragón, G., Otálora, M. A. G. Lutzoni, F. (2010) Phylogenetic study of Catapyrenium s. str. (Verrucariaceae, lichen-forming Ascomycota) and related genus Placidiopsis . Mycologia 102: 291–304.CrossRefGoogle ScholarPubMed
Randlane, T., Jüriado, I., Suija, A., Lõhmus, P. Leppik, E. (2008) Lichens in the new Red List of Estonia. Folia Cryptogamica Estonica 44: 113–120.Google Scholar
Rozen, S. Skaletsky, H. (2000) Primer3 on the WWW for general users and for biologist programmers. Methods in Molecular Biology 132: 365–386.Google Scholar
Rudi, K., Skulberg, O. M. Jakobsen, K. S. (1998) Evolution of cyanobacteria by exchange of genetic material among phyletically related strains. Journal of Bacteriology 180: 3453–3461.Google Scholar
Scheidegger, C., Bilovitz, P. O., Werth, S., Widmer, I. Mayrhofer, H. (2012) Hitchhiking with forests: population genetics of the epiphytic lichen Lobaria pulmonaria in primeval and managed forests in southeastern Europe. Ecology and Evolution 2: 2223–2240.Google Scholar
Sérusiaux, E. (1989) Liste Rouge des Macrolichens dans la Communauté Européene. Liège: Centre de Recherches sur les Lichens, Département de Botanique.Google Scholar
Smith, C. W., Aptroot, A., Coppins, B. J., Fletcher, A., Gilbert, O. L., James, P. W. Wolseley, P. A. (eds) (2009) The Lichens of Great Britain and Ireland. London: British Lichen Society.Google Scholar
Werth, S. (2010) Population genetics of lichen-forming fungi – a review. Lichenologist 42: 499–519.Google Scholar
Werth, S., Cornejo, C. Scheidegger, C. (2013) Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae). Applications in Plant Sciences 1: 1200290.CrossRefGoogle ScholarPubMed
White, T. J., Bruns, T., Lee, S. Taylor, J. W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications (M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White, eds): 315–322. New York: Academic Press.Google Scholar