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In vitro culturing and resynthesis of the mycobiont Protoparmeliopsis muralis with algal bionts

Published online by Cambridge University Press:  08 January 2013

Beata GUZOW-KRZEMIŃSKA  and
Elfie STOCKER-WÖRGÖTTER
Beata GUZOW-KRZEMIŃSKA
Affiliation:
Department of Molecular Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland. Email: beatagk@biotech.ug.gda.pl Department of Organismic Biology, University of Salzburg, Hellbrunner Str. 34, 5020 Salzburg, Austria
Elfie STOCKER-WÖRGÖTTER
Affiliation:
Department of Organismic Biology, University of Salzburg, Hellbrunner Str. 34, 5020 Salzburg, Austria
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Abstract

The widespread and ubiquitous lichen-forming fungus Protoparmeliopsis muralis is able to form a thallus with Trebouxia species. In this study, several photobiont strains were isolated from different specimens of P. muralis and cultured in vitro. The compatibility of Trebouxia spp. and Asterochloris algae with P. muralis were investigated in in vitro resynthesis experiments and the re-lichenized bionts were observed with the scanning electron microscope. It was found that, in addition to compatible photobionts, also a presumably incompatible Asterochloris sp. was able to interact with the mycobiont. The life strategy that enables the mycobiont to form associations with a wider range of photobionts could be advantageous for the survival of the lichen and successful colonization of new habitats.

Keywords

compatibility of biontsITS rDNA sequencinglichenphotobiontscanning electron microscopy
Type
Articles
Information
The Lichenologist , Volume 45 , Issue 1 , January 2013 , pp. 65 - 76
Copyright
Copyright © British Lichen Society 2013

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