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In vivo stability of protein coatings on poly lactic co glycolic nanoparticles

Published online by Cambridge University Press:  17 June 2016

Jordi Llop ,
Marco Marradi ,
Pengfei Jiang ,
Vanessa Gómez-Vallejo ,
Zuriñe Baz ,
María Echeverría ,
Changyou Gao  and
Sergio E. Moya
Jordi Llop
Affiliation:
Radiochemistry and Nuclear Imaging Group, CIC biomaGUNE, San Sebastián, Spain.
Marco Marradi
Affiliation:
Soft Mater Nanotechnology Laboratory, CIC biomaGUNE, San Sebastián, Spain.
Pengfei Jiang
Affiliation:
MOE Key Laboratory of Macrmolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
Vanessa Gómez-Vallejo
Affiliation:
Radiochemistry Platform, CIC biomaGUNE, San Sebastián, Spain.
Zuriñe Baz
Affiliation:
Radiochemistry and Nuclear Imaging Group, CIC biomaGUNE, San Sebastián, Spain.
María Echeverría
Affiliation:
Soft Mater Nanotechnology Laboratory, CIC biomaGUNE, San Sebastián, Spain.
Changyou Gao
Affiliation:
MOE Key Laboratory of Macrmolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
Sergio E. Moya*
Affiliation:
Soft Mater Nanotechnology Laboratory, CIC biomaGUNE, San Sebastián, Spain.
*
*(Email: smoya@cicbiomagune.es)
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Abstract

Submicron-sized poly(lactide-co-glycolide) nanoparticles (PLGA-NPs) stabilised with bovine serum albumin (BSA) are dual radiolabelled using gamma emitters with different energy spectra incorporated into the core and coating. PLGA core is labelled by encapsulation of 111In-doped iron oxide NPs inside PLGA-NPs during NP preparation, while the BSA coating is labelled by electrophilic substitution using 125I. After intravenous administration into rats, energy-discriminant single-photon emission computerised tomography (SPECT) resolved each radioisotope independently. Imaging revealed different fates for the core and coating, with a fraction of the two radionuclides co-localising in the liver and lungs for long periods of time after administration, suggesting that NPs are stable in these organs. The general methodology reported here represents an excellent alternative for visualising the degradation process of multi-labelled NPs in vivo and can be extended to a wide range of engineered NPs.

Keywords

biomaterialpolymernanoscale
Type
Articles
Information
MRS Advances , Volume 1 , Issue 56: Biomaterials and Soft Materials/Engaged Learning of Materials Science and Engineering in the 21st Century , 2016 , pp. 3767 - 3773
Copyright
Copyright © Materials Research Society 2016 

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