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Ångström- and Nano-scale Pore-Based Nucleic Acid Sequencing of Current and Emergent Pathogens

Published online by Cambridge University Press:  29 October 2020

Britney A Shepherd ,
Md Rubayat-E Tanjil ,
Yunjo Jeong ,
Bilgenur Baloğlu ,
Jingqiu Liao  and
Michael Cai Wang Open the ORCID record for Michael Cai Wang [Opens in a new window]
Britney A Shepherd
Affiliation:
Department of Medical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida33620, USA.
Md Rubayat-E Tanjil
Affiliation:
Department of Mechanical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida33620, USA.
Yunjo Jeong
Affiliation:
Department of Mechanical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida33620, USA.
Bilgenur Baloğlu*
Affiliation:
Centre for Biodiversity Genomics, University of Guelph, 50 Stone Road East, Guelph, OntarioN1G2W1, Canada.
Jingqiu Liao*
Affiliation:
Department of Systems Biology, Columbia University, 1130 St. Nicholas Avenue, New York, New York10032, USA.
Michael Cai Wang*
Affiliation:
Department of Medical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida33620, USA. Department of Mechanical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida33620, USA.
*
†Author to whom correspondence should be addressed. MCW:mcwang@usf.eduBB:bbaloglu@uoguelph.caJL:jl5897@cumc.columbia.edu
†Author to whom correspondence should be addressed. MCW:mcwang@usf.eduBB:bbaloglu@uoguelph.caJL:jl5897@cumc.columbia.edu
†Author to whom correspondence should be addressed. MCW:mcwang@usf.eduBB:bbaloglu@uoguelph.caJL:jl5897@cumc.columbia.edu

Abstract

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State-of-the-art nanopore sequencing enables rapid and real-time identification of novel pathogens, which has wide application in various research areas and is an emerging diagnostic tool for infectious diseases including COVID-19. Nanopore translocation enables de novo sequencing with long reads (> 10 kb) of novel genomes, which has advantages over existing short-read sequencing technologies. Biological nanopore sequencing has already achieved success as a technology platform but it is sensitive to empirical factors such as pH and temperature. Alternatively, ångström- and nano-scale solid-state nanopores, especially those based on two-dimensional (2D) membranes, are promising next-generation technologies as they can surpass biological nanopores in the variety of membrane materials, ease of defining pore morphology, higher nucleotide detection sensitivity, and facilitation of novel and hybrid sequencing modalities. Since the discovery of graphene, atomically-thin 2D materials have shown immense potential for the fabrication of nanopores with well-defined geometry, rendering them viable candidates for nanopore sequencing membranes. Here, we review recent progress and future development trends of 2D materials and their ångström- and nano-scale pore-based nucleic acid (NA) sequencing including fabrication techniques and current and emerging sequencing modalities. In addition, we discuss the current challenges of translocation-based nanopore sequencing and provide an outlook on promising future research directions.

Type
Articles
Information
MRS Advances , Volume 5 , Issue 56: Materials advances in application to COVID-19 related challenges , 2020 , pp. 2889 - 2906
Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
Copyright © The Author(s), 2020, published on behalf of Materials Research Society by Cambridge University Press

Footnotes

*

These authors contributed equally to this work.

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