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Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation

Published online by Cambridge University Press:  17 February 2014

Yoshikazu Kumashiro ,
Jun Ishihara ,
Terumasa Umemoto ,
Kazuyoshi Itoga ,
Jun Kobayashi ,
Masayuki Yamato  and
Teruo Okano
Yoshikazu Kumashiro
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Jun Ishihara
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan Division of Cellular Therapy, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8039, Japan.
Terumasa Umemoto
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Kazuyoshi Itoga
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Jun Kobayashi
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Masayuki Yamato
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Teruo Okano
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
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Abstract

Thready stripe-patterned thermo-responsive surfaces were prepared and their surface properties were characterized. Prepared 3 μm wide stripe-patterned surfaces were evaluated by observing the adhesions and detachments of three types of cells: HeLa cells (HeLas), human umbilical vein endothelial cells (HUVECs), and NIH-3T3 cells (3T3s). Although cell adhesion and detachment in response to temperature were observed on all cells on a conventional thermo-responsive surface without patterns, the thermo-responsive surface with a 3 μm striped-pattern exhibited various cell adhesion properties. HeLas hardly adhered to the patterned surface even at 37 °C. On the other hand, although HUVECs adhered on the patterned surface at 12 h after incubation at 37 °C, the adhered HUVECs detached themselves after another 12 h incubation at 37 °C. 3T3s adhered to the patterned surface at 37 °C and detached themselves after reducing temperature to 20 °C. A mixture of HeLa, HUVEC and 3T3 was separated using their different specific cell-adhesion properties, and the composition of cells was analyzed by a flow-cytometry. As a result, the conventional thermo-responsive surface with a stripe-pattern was found to function as a cell-separating interface by using specific cell adhesion properties.

Keywords

biomaterialmicrostructurethin film
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 107 - 112
Copyright
Copyright © Materials Research Society 2014 

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References

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