Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-27T18:11:09.481Z Has data issue: false hasContentIssue false
  • English
  • Français

Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation

Published online by Cambridge University Press:  17 February 2014

Yoshikazu Kumashiro ,
Jun Ishihara ,
Terumasa Umemoto ,
Kazuyoshi Itoga ,
Jun Kobayashi ,
Masayuki Yamato  and
Teruo Okano
Yoshikazu Kumashiro
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Jun Ishihara
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan Division of Cellular Therapy, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8039, Japan.
Terumasa Umemoto
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Kazuyoshi Itoga
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Jun Kobayashi
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Masayuki Yamato
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Teruo Okano
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Get access

Abstract

Thready stripe-patterned thermo-responsive surfaces were prepared and their surface properties were characterized. Prepared 3 μm wide stripe-patterned surfaces were evaluated by observing the adhesions and detachments of three types of cells: HeLa cells (HeLas), human umbilical vein endothelial cells (HUVECs), and NIH-3T3 cells (3T3s). Although cell adhesion and detachment in response to temperature were observed on all cells on a conventional thermo-responsive surface without patterns, the thermo-responsive surface with a 3 μm striped-pattern exhibited various cell adhesion properties. HeLas hardly adhered to the patterned surface even at 37 °C. On the other hand, although HUVECs adhered on the patterned surface at 12 h after incubation at 37 °C, the adhered HUVECs detached themselves after another 12 h incubation at 37 °C. 3T3s adhered to the patterned surface at 37 °C and detached themselves after reducing temperature to 20 °C. A mixture of HeLa, HUVEC and 3T3 was separated using their different specific cell-adhesion properties, and the composition of cells was analyzed by a flow-cytometry. As a result, the conventional thermo-responsive surface with a stripe-pattern was found to function as a cell-separating interface by using specific cell adhesion properties.

Keywords

biomaterialmicrostructurethin film
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 107 - 112
Copyright
Copyright © Materials Research Society 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Gu, Z.-Z., Uetsuka, H., Takahashi, K., Nakajima, R., Onishi, H., Fujishima, A., and Sato, O., Angew. Chem. Int. Ed. 42, 894 (2003).CrossRefGoogle Scholar
Patankar, N. A., Langmuir 20, 8209 (2004).CrossRefGoogle Scholar
Yu, X., Wang, Z., Jiang, Y., Shi, F., and Zhang, X., Adv. Mater. 17, 1289 (2005).CrossRefGoogle Scholar
Tauk, L., Schröder, A. P., Decher, G., and Giuseppone, N., Nat Chem 1, 649 (2009).CrossRefGoogle Scholar
Chen, L., Liu, M., Bai, H., Chen, P., Xia, F., Han, D., and Jiang, L., J. Am. Chem. Soc. 131, 10467 (2009).CrossRefGoogle Scholar
Jiang, Y., Wan, P., Smet, M., Wang, Z., and Zhang, X., Adv. Mater. 20, 1972 (2008).CrossRefGoogle Scholar
Ge, H., Wang, G., He, Y., Wang, X., Song, Y., Jiang, L., and Zhu, D., ChemPhysChem 7, 575 (2006).CrossRefGoogle Scholar
Masuda, T., Hidaka, M., Murase, Y., Akimoto, A. M., Nagase, K., Okano, T., and Yoshida, R., Angew. Chem. Int. Ed. 52, 7468 (2013).CrossRefGoogle Scholar
Diebold, U., Surf. Sci. Rep. 48, 53 (2003).CrossRefGoogle Scholar
Yamato, M., and Okano, T., Mater. Today 7, 42 (2004).CrossRefGoogle Scholar
Kikuchi, A., and Okano, T., J. Controlled Release 101, 69 (2005).CrossRefGoogle Scholar
Kumashiro, Y., Yamato, M., and Okano, T., Ann. Biomed. Eng. 38, 1977 (2010).CrossRefGoogle Scholar
Akiyama, Y., Kikuchi, A., Yamato, M., and Okano, T., Langmuir 20, 5506 (2004).CrossRefGoogle Scholar
Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Yamamoto, K., Adachi, E., Nagai, S., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T., and Tano, Y., N. Engl. J. Med. 351, 1187 (2004).CrossRefGoogle Scholar
Kumashiro, Y., Itoga, K., Kinoshita, Y., Yamato, M., and Okano, T., Chem. Lett. 42, 741 (2013).CrossRefGoogle Scholar
Kumashiro, Y., Matsunaga, T., Muraoka, M., Tanaka, N., Itoga, K., Kobayashi, J., Tomiyama, Y., Kuroda, M., Shimizu, T., Hashimoto, I., Umemura, K., Yamato, M., and Okano, T., J. Biomed. Mater. Res. A, (2013) in press.Google Scholar