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Biomimetic Mineralization of an Aligned, Self-Assembled Collagenous Matrix.

Published online by Cambridge University Press:  21 February 2011

David Christiansen  and
Frederick H. Silver
David Christiansen
Affiliation:
Department of Pathology, UMDNJ - Robert Wood Johnson Medical School, Piscataway, NJ 08854
Frederick H. Silver
Affiliation:
Department of Pathology, UMDNJ - Robert Wood Johnson Medical School, Piscataway, NJ 08854
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Abstract

An in-vitro method of mineralizing an aligned, self-assembled collagenous matrix is presented. Reconstituted collagen fibers were mineralized by exposure to saturated solutions of calcium and phosphate of varying pH in a double diffusion chamber for seven days at room temperature. Microscopic investigation of the mineral precipitate within the fibers indicate the formation of hydroxyapatite crystals with features comparable to mineral observed in bone and avian tendon. Mechanical test results indicate that tensile strength and tangent modulus increase after mineralization in comparison to unmineralized control fibers. These results suggest that mineralization of collagen fiber in-vitro may parallel some of the events seen in mineralization of bone and turkey tendon. In addition, mineralized collagen fibers may be useful in the design of composites for the replacement or augmentation of hard tissue

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 255: Symposium Z – Hierarchically Structured Materials , 1991 , 367
Copyright
Copyright © Materials Research Society 1992

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