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Cells in Micropatterned Hydrogels: Applications in Biosensing

Published online by Cambridge University Press:  01 February 2011

Won-Gun Koh  and
Michael Pishko
Won-Gun Koh
Affiliation:
Department of Chemical Engineering, The Pennsylvania University, 104 Fenske, University Park, PA 16802-4420
Michael Pishko
Affiliation:
Department of Chemical Engineering, The Pennsylvania University, 104 Fenske, University Park, PA 16802-4420
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Abstract

Here we will discuss the development of arrays of mammalian cells of differing phenotype integrated with microfluidics and microsensors for applications such as drug screening and used to monitor cellular effects of multiple chemical and biological candidates. To fabricate these arrays, we immobilized either single or small groups of cells in 3-dimensional poly(ethylene glycol) hydrogel microstructures fabricated on plastic or glass surfaces. These microstructures were created using either photolithography or printed using microarray robots. The resulting hydrogel microstructures were fabricated to dimensions as small as 10 microns in diameter with aspect ratios as high as 1.4. The gels were highly swollen with water to permit mass transfer of nutrients and potential analytes to the cells, and cell adhesion molecules were immobilized in the gel to allow cell attachment and spreading. Cell viability was confirmed using fluorescent assays and ESEM used to verify complete cell encapsulation. The specific and non-specific response of these cells to target molecules was monitored using optical or electrochemical detectors and analyzed to quantify the effect of these agents on the different phenotypes present in the array.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 723: Symposia M/O – Molecularly Imprinted Materials – Chemical and Biological Sensors-Materials and Devices , 2002 , O5.5
Copyright
Copyright © Materials Research Society 2002

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