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Cellulase Production from Filamentous Fungi for Its Application in the Hydrolysis of Wheat Straw

Published online by Cambridge University Press:  18 February 2015

L. Toscano-Palomar ,
G. Montero-Alpirez ,
M. Stilianova-Stoytcheva ,
E. Vertiz-Pelaez  and
y E. Romero Uscanga
L. Toscano-Palomar*
Affiliation:
Instituto Tecnológico de Mexicali, México.
G. Montero-Alpirez
Affiliation:
Instituto de Ingeniería, UABC, México.
M. Stilianova-Stoytcheva
Affiliation:
Instituto de Ingeniería, UABC, México.
E. Vertiz-Pelaez
Affiliation:
Instituto Tecnológico de Mexicali, México.
y E. Romero Uscanga
Affiliation:
Instituto de Ingeniería, UABC, México.
*
*Presenting author’s email: toscano.lydia@itmexicali.edu.mx
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Abstract

Extended research has been developed in the use of wheat straw (WS) as biomass for the production of biofuels (bioethanol), including the processes of degradation of cellulose by enzymatic systems. For centuries, Cellulose has been used by man; however, its enormous potential as a renewable energy source was recognized only after the discovery of cellulose degrading enzymes (cellulases). A wide variety of microorganisms can produce cellulolytic enzymes under appropriate culture conditions and among these microorganisms are filamentous fungi of the genera Trichoderma, Aspergillus, Penicillium and Fusarium. The purpose of this study was to produce cellulase enzyme from previously isolated and characterized filamentous fungi. Cellulytic fungi belonged to Aspergillus flavus, Aspergillus niger, Aspergillus oryzae, Penicillium chrysogenum, Penicillium sp., and Trichoderma harzianum. All these strains were preserved by lyophilization and also kept in sterile media (sand and soil) at 4 °C. The production of cellulases by submerged fermentation was performed in a Mandels mineral medium. The nitrogen sources were urea and ammonium sulfate. Glucose alone was used in the pre-inoculum, and dried and ground wheat straw was used in the fermentation as carbon sources. Subcultures of spore suspensions were incubated with orbital stirring (120 rpm) at 30 °C for 48 hours and used as inoculum for submerged fermentation with wheat straw as substrate in mineral medium with an initial pH of 5. Activity cellulase was determined by the method of 3,5-dinitrosalicylic acid (DNS). The results showed that wheat straw have potential for use as a substrate in the production of cellulases. Aspergillus niger showed the highest enzymatic activity from the cellulase produced 0.051 FPU (filter paper units) after 96 hours of fermentation.

Keywords

Cellulasefilamentous fungisubmerged fermentation and enzymatic activity
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1763: Symposium 2B – Materials for Biosensor Applications , 2015 , IMRC2014-S2B-P031
Copyright
Copyright © Materials Research Society 2015 

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References

REFERENCES

Kuhad, R. C. and Gupta, R., Singh, A. (2011) Microbial cellulase and their industrial applications.. Enzyme Research, 10. Available: http://dx.doi.org/10.4061/2011/280696 Google Scholar
Klein-Marcuschamer, D. and Oleskowicz-Popiel, P., Simmons, B.A., Blanch, H.W., “The challenge of enzyme cost in the production of lignocellulosic biofuels,” Biotechnology and Bioengineering, vol. 109, pp. 1083–1087, 2012.CrossRefGoogle Scholar
Qureshi, N. and Saha, B. C., Dien, B.C., Hector, R.E., Cotta, M.A., “Production of butanol (a biofuel) from agricultural residues: Part I - Use of barkey straw hydrolysate,” Biomass and Bioenergy vol. 34, pp. 559–565, 2010.CrossRefGoogle Scholar
Binod, P. and Janu, K. U., Sindhu, R., Pandey, A., “Hydrolysis of Lignocellulosic Biomass for Bioethanol Production,” in BIOFUELS.- Alternative Feedstocks and Conversion Processes, Pandey, A. and Larroche, C., Ricke, S.C., Dussap, C.G., Gnansounou, E., Eds., ed: Academic Press, 2011, pp. 229–250.Google Scholar
(SIAP). (2013) Produccion Agropecuaria Anual. Available: http://www.siap.sagarpa.gob.mx/ Google Scholar
Coronado, M. A. and Montero, G., García, C., Perez, A., Perez, L. (2012, Emisiones de las quemas de residuos agrícolas en el Valle de Mexicali, 1987-2010 Rev. Int. Cont. Ambie. 28 Sup. (1), 117–124. Available: http://www.journals.unam.mx/index.php/rica/article/viewFile/34938/31885 Google Scholar
AOAC., “Official Methods of Analysis.,” 17 ed. Washington, D.C. USA: Association of Official Agricultural Chemists, Incorporated, 2002.Google Scholar
Morris, B. and Jacob, S., The Chemical Analysis of Foods and Food Products, 2nd ed. New York: D. Van Nostrand Company,, 1938.Google Scholar
Mandels, M. and Reese, E. T., “Induction of cellulase in Trichoderma viride as influenced by carbon sources and metals,” Journal of Bacteriology, vol. 73, pp. 269–278, 1957.Google ScholarPubMed
Ghose, T. K., “Measurement of Cellulase Activities,” Pure & Appl. Chem., vol. 59, pp. 257–268, 1987.CrossRefGoogle Scholar
Ahmed, I. and Anjum Zia, M., Nazir Iqbal, H.M., “Bioprocessing of proximally analyzed wheat straw for enhanced cellulase production through process optimization with Trichoderrma viride under SSF,” International Journal of Biological and Life Sciences, vol. 6, pp. 164–170, 2010.Google Scholar
Coughlan, M. P., “The properties of fungal and bacterial cellulases with comment on their production and application,” Biotechnology & Genetic Engineering Reviews, vol. 3, pp. 39–109, 1985.CrossRefGoogle Scholar
Da Silva, P. and Sanchez, C., Da Silva, J., Da Cruz, J.G., “Experimental mixture design as a tool to enhance glycosyl hydrolases production by a new Trichoderma harzianum P49P11 strain cultivated under controlled bioreactor submerged fermentation,” Bioresource Technology, vol. 132, pp. 401–405, 2013.CrossRefGoogle Scholar
Kannan, M. R. and Sajith, S., Sreedevi, S., Priji, P., Unni, K.N., Sarath Josh, M.K., Jisha, V.N., Pradeep, S., Benjamin, S., “Lignocellulolytic activities of a novel strain of Trichoderma harzianum ,” Advances in Bioscience and Biotechnology, vol. 4, pp. 214–221, 2013.Google Scholar
Mrudula, S. and Murugammal, R., “Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate,” Brazilian Journal of Microbiology vol. 42, pp. 1119–1127, 2011.CrossRefGoogle ScholarPubMed
Bansal, N. and Tewari, R., Soni, R., Soni, S.K., “Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues,” Waste Management, vol. 32, pp. 1341–1346, 2012.CrossRefGoogle ScholarPubMed
Kocher, G. and Kalra, K., Banta, G., “Optimization of cellulase production by submerged fermentation of rice straw by Trichoderma harzianum RUT 8230 ,” The Internet Journal of Microbiology, vol. 5, 2007.Google Scholar
Shahriari, M. and Ramanan, R., Wahab, M., Mohamad, R., Mustafa, S.,Ariff, A., “Improved cellulase production by Aspergillus terreus using oil palm empty fruit bunch fibre as substrate in a stirred tank bioreactor through optimization of the fermentation conditions,” Australian Journal of Basic & Applied Science, vol. 4, 2010.Google Scholar
Singh, A. and Singh, N., Bishnoi, N.R., “Production of cellulases by Aspergillus heteromorphus from wheat straw under submerged fermentation,”International Journal of Civil and Enviromental Engineering, vol. 1, pp. 23–26, 2009.Google Scholar
Shobana, P. and Maheswari, N. U., “Production of cellulase from Aspergillus fumigatus under submerged and solid state fermentation using agricultural waste,” International Journal of Advances in Pharmacy, Biology and Chemistry, vol. 2, pp. 595–599, 2013.Google Scholar