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Controlled Macrophage Adhesion on Micropatterned Hydrogel Surfaces

Published online by Cambridge University Press:  01 February 2011

P. Krsko ,
K. Vartanian ,
H. Geller  and
M. Libera
P. Krsko
Affiliation:
Stevens Institute of Technology, Hoboken, NJ
K. Vartanian
Affiliation:
NIH NHLBI, Bethesda, MD
H. Geller
Affiliation:
NIH NHLBI, Bethesda, MD
M. Libera
Affiliation:
Stevens Institute of Technology, Hoboken, NJ
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Abstract

We studied the protein adsorption and subsequent macrophage adhesion on poly(ethylene glycol) [PEG] hydrogels crosslinked using a focused electron beam. Thin-film gels were patterned on silicon substrates and could be formed with swell ratios (hydrated height/ dry height) anywhere between fifteen and unity. We have shown that laminin does not adsorb onto highly swelling gels but that it does adsorb on heavily-crosslinked low-swelling gels. As part of ongoing research on patterning surfaces to control neurite growth in the context of the inflammatory environment of a spinal cord injury, we are interested in how these gel surfaces interact with macrophages. We show that the high-swelling PEG gels resist macrophage adhesion, but the macrophages adhere to low-swelling gels pre-exposed to laminin. By spatially patterning combinations of low and high swelling gels, we show that macrophage adhesion can be confined to specific locations on a surface.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA8.7
Copyright
Copyright © Materials Research Society 2005

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References

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