Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-25T00:29:39.721Z Has data issue: false hasContentIssue false
  • English
  • Français

Controlled Macrophage Adhesion on Micropatterned Hydrogel Surfaces

Published online by Cambridge University Press:  01 February 2011

P. Krsko ,
K. Vartanian ,
H. Geller  and
M. Libera
P. Krsko
Affiliation:
Stevens Institute of Technology, Hoboken, NJ
K. Vartanian
Affiliation:
NIH NHLBI, Bethesda, MD
H. Geller
Affiliation:
NIH NHLBI, Bethesda, MD
M. Libera
Affiliation:
Stevens Institute of Technology, Hoboken, NJ
Get access

Abstract

We studied the protein adsorption and subsequent macrophage adhesion on poly(ethylene glycol) [PEG] hydrogels crosslinked using a focused electron beam. Thin-film gels were patterned on silicon substrates and could be formed with swell ratios (hydrated height/ dry height) anywhere between fifteen and unity. We have shown that laminin does not adsorb onto highly swelling gels but that it does adsorb on heavily-crosslinked low-swelling gels. As part of ongoing research on patterning surfaces to control neurite growth in the context of the inflammatory environment of a spinal cord injury, we are interested in how these gel surfaces interact with macrophages. We show that the high-swelling PEG gels resist macrophage adhesion, but the macrophages adhere to low-swelling gels pre-exposed to laminin. By spatially patterning combinations of low and high swelling gels, we show that macrophage adhesion can be confined to specific locations on a surface.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA8.7
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Auger, M.J. and Ross, J.A., “The Biology of the Macrophage,” The Macrophage, ed. Lewis, C.E. and McGee, J.O.D. (Oxford University Press, Oxford, 1992), pp. 3–73.Google Scholar
2. Riches, D., “Macrophage Involvement in Wound Repair,” The Molecular and Cellular Biology of Wound Repair, ed. Clark, R. (Plenum, New York, 1996), pp. 95–131.Google Scholar
3. Collier, T., Anderson, J., J., , et al., Biomed. Mat. Res. 69A, 644 (2004).Google Scholar
4. Jenney, C. and Anderson, J., J. Biomed. Mat. Res. 44, 206–216 (1999).Google Scholar
5. Ghosh, P., et al., Angew. Chem. Int. Ed. 38, 1592–1595 (1999).Google Scholar
6. Kao, W. and Hubell, J., Biotechnology and Bioengineering 59, 2–9 (1999).Google Scholar
7. Kao, W.J., Biomaterials 20, 2213–2221 (1999).Google Scholar
8. Wagner, V. and Bryers, J., J. Biomed. Mat. Res. 66A, 62–78 (2003).Google Scholar
9. Krsko, P., Mansfield, M., Sukhishvili, S., Libera, M. and Clancy, R., Langmuir 19, 5618–5625 (2003).Google Scholar
10. Wojciak-Stothard, B., Curtis, A., Monaghan, W., Macdonald, K. and Wilkinson, C., Exp. Cell Res. 223, 426–435 (1996).Google Scholar
11. Hong, Y., Krsko, P. and Libera, M., Langmuir, in press, 2004 Google Scholar