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Immobilization of oligonucleotide-functionalized magnetic nanobeads in DNA-coils studied by electron microscopy and atomic force microscopy

Published online by Cambridge University Press:  19 July 2011

Mattias Strömberg ,
Sultan Akhtar ,
Klas Gunnarsson ,
Camilla Russell ,
David Herthnek ,
Peter Svedlindh ,
Mats Nilsson ,
Maria Strømme  and
Klaus Leifer
Mattias Strömberg
Affiliation:
Department of Engineering Sciences, Uppsala University, Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Sultan Akhtar
Affiliation:
Department of Engineering Sciences, Uppsala University, Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Klas Gunnarsson
Affiliation:
Department of Engineering Sciences, Uppsala University, Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Camilla Russell
Affiliation:
Department of Immunology, Genetics and Pathology, Uppsala University, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden.
David Herthnek
Affiliation:
Department of Immunology, Genetics and Pathology, Uppsala University, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden.
Peter Svedlindh
Affiliation:
Department of Engineering Sciences, Uppsala University, Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Mats Nilsson
Affiliation:
Department of Immunology, Genetics and Pathology, Uppsala University, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden.
Maria Strømme
Affiliation:
Department of Engineering Sciences, Uppsala University, Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Klaus Leifer
Affiliation:
Department of Engineering Sciences, Uppsala University, Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
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Abstract

Immobilization of oligonucleotide-functionalized magnetic nanobeads by hybridization in DNA-coils formed by rolling circle amplification has been investigated using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The TEM results supported earlier made observations that small beads with low oligonucleotide surface coverage preferably immobilize in the interior of the DNA-coils and do not tend to link several DNA-coils together whereas large beads with high surface coverage to a larger extent connect several DNA-coils together to clusters of several DNA-coils with beads. AFM provided direct visualization of the DNA-coils as thread-like objects. DNA-coils with immobilized beads appeared as a collection of beads with thread-like features in their near vicinity.

Keywords

nanoscaletransmission electron microscopy (TEM)colloid
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1355: Symposium JJ – Biological Hybrid Materials for Life Sciences , 2011 , mrss11-1355-jj05-08
Copyright
Copyright © Materials Research Society 2011

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References

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